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Raster-Scanning Optoacoustic Mesoscopy (RSOM) overcomes this limitation by utilizing the intrinsic optical absorption contrast based on the optoacoustic effect. We show that RSOM is able to provide fast quantitative visualization of the neuromelanin content in intact large organoids at single cell resolution.
The system is based on a reflection-mode implementation of RSOM. The detectors used are custom made, ultrawideband, and spherically focused. The use of such detectors enables light coupling from the same side as the detector, thus reflection-mode. Light is in turn coupled using a fiber bundle, and the detector is raster scanned in the xy-plane. Subsequently, to retrieve small features, the raw data are reconstructed using a multi-bandwidth, beamforming reconstruction algorithm.
Comparison of the system performance at the different frequencies shows as expected a higher resolution in case of the 100 MHz detector compared to the 50 MHz. On the other hand the 50 MHz has a better SNR, can detect features from deeper layers, and has higher angular acceptance. Based on these characteristics the 50 MHz detector was mostly used. After comparing the performance we monitored the growth of B16F10 cells, melanin tumor, over the course of 9 days. We see correspondence between the optoacoustic measurements and the cryoslice validations. Additionally, in areas close to the tumor we see sprouting of new vessels, starting at day 4-5, which corresponds to tumor angiogenesis.
Modeling the shape of cylindrically focused transducers in three-dimensional optoacoustic tomography
Modeling the shape of cylindrically focused transducers in three-dimensional optoacoustic tomography