Prof. Alicia A. Petryk Profile

Prof. Alicia A. Petryk

Assistant Professor at Univ of Bridgeport

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Author

Publications (21)

Proceedings Article | 22 February 2017 Paper

Hypo-fractionated radiation, magnetic nanoparticle hyperthermia and a viral immunotherapy treatment of spontaneous canine cancer

P. Jack Hoopes, Karen Moodie, Alicia Petryk, James Petryk, Shawntel Sechrist, David Gladstone, Nicole Steinmetz, Frank Veliz, Alicea Bursey, Robert Wagner, Ashish Rajan, Danielle Dugat, Margaret Crary-Burney, Steven Fiering

Proceedings Volume 10066, 1006605 (2017) https://doi.org/10.1117/12.2256213

KEYWORDS: Tumors, Proteins, Computed tomography, Nanoparticles, Melanoma, Magnetism, Cancer, Oncology, Tissues, Radiotherapy, Modulation

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Proceedings Article | 13 March 2015 Paper

SWIFT-MRI imaging and quantitative assessment of IONPs in murine tumors following intra-tumor and systemic delivery

Russell Reeves, Alicia Petryk, Elliot Kastner, Jinjin Zhang, Hattie Ring, Michael Garwood, P. Jack Hoopes

Proceedings Volume 9326, 93260Q (2015) https://doi.org/10.1117/12.2083129

KEYWORDS: Tumors, Iron, Tissues, Magnetic resonance imaging, Nanoparticles, Magnesium, Magnetism, Imaging systems, Transmission electron microscopy, In vivo imaging

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Proceedings Article | 13 March 2015 Paper

Utility and translatability of mathematical modeling, cell culture and small and large animal models in magnetic nanoparticle hyperthermia cancer treatment research

P. Hoopes, Alicia Petryk, Adwiteeya Misra, Elliot Kastner, John Pearce, Thomas Ryan

Proceedings Volume 9326, 932604 (2015) https://doi.org/10.1117/12.2084285

KEYWORDS: Mathematical modeling, Nanoparticles, Cancer, Magnetism, Animal model studies, Tissues, Oncology, Iron, Tumor growth modeling, Oxides

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Proceedings Article | 13 March 2015 Paper

Alicia Petryk, Adwiteeya Misra, Elliot Kastner, Courtney Mazur, James Petryk, P. Jack Hoopes

Proceedings Volume 9326, 93260K (2015) https://doi.org/10.1117/12.2084041

KEYWORDS: Tumors, Tissues, Magnetism, Nanoparticles, Cancer, Particles, Oncology, Iron, Ionizing radiation, Blood circulation

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Proceedings Article | 12 March 2015 Paper

Magnetic nanoparticle hyperthermia cancer treatment efficacy dependence on cellular and tissue level particle concentration and particle heating properties

Alicia Petryk, Adwiteeya Misra, Courtney Mazur, James Petryk, P. Hoopes

Proceedings Volume 9326, 93260L (2015) https://doi.org/10.1117/12.2083432

KEYWORDS: Nanoparticles, Iron, Magnetism, Particles, Cancer, In vitro testing, Microelectromechanical systems, Oncology, Tissues, Tumors

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Proceedings Article | 12 March 2015 Paper

Alternating magnetic field optimization for IONP hyperthermia cancer treatment

Elliot Kastner, Russell Reeves, William Bennett, Adwiteeya Misra, Jim Petryk, Alicia Petryk, P. Jack Hoopes

Proceedings Volume 9326, 93260M (2015) https://doi.org/10.1117/12.2083196

KEYWORDS: Tissues, Tumors, Nanoparticles, Temperature metrology, Magnetism, Synthetic aperture radar, Infrared cameras, Cancer, Oncology, Infrared imaging

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Proceedings Article | 12 March 2015 Paper

Macroscopic and microscopic biodistribution of intravenously administered iron oxide nanoparticles

Adwiteeya Misra, Alicia Petryk, Rendall Strawbridge, P. Jack Hoopes

Proceedings Volume 9326, 93260N (2015) https://doi.org/10.1117/12.2082989

KEYWORDS: Iron, Magnesium, Nanoparticles, Kidney, Lymphatic system, Heart, Liver, Brain, Lung, Toxicity

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Proceedings Article | 12 March 2015 Paper

Effect of radiation energy and intracellular iron dose on iron oxide nanoparticle enhancement of radiation cytotoxicity

Courtney Mazur, Rendall Strawbridge, Ella Thompson, Alicia Petryk, David Gladstone, P. Jack Hoopes

Proceedings Volume 9326, 93260P (2015) https://doi.org/10.1117/12.2082550

KEYWORDS: Iron, Tumors, Radiation effects, Magnesium, Nanoparticles, Oxides, In vivo imaging, Cancer, Radiotherapy, Particles

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Iron oxide nanoparticles (IONPs) are one of several high-Z materials currently being investigated for their ability to enhance the cytotoxic effects of therapeutic ionizing radiation. Studies with iron oxide, silver, gold, and hafnium oxide suggest radiation dose, radiation energy, cell type, and the type and level of metallic nanoparticle are all critical factors in achieving radiation enhancement in tumor cells. Using a single 4 Gy radiation dose, we compared the level of tumor cell cytotoxicity at two different intracellular iron concentrations and two different radiation energies in vitro. IONPs were added to cell culture media at concentrations of 0.25 mg Fe/mL and 1.0 mg Fe/mL and incubated with murine breast adenocarcinoma (MTG-B) cells for 72 hours. Extracellular iron was then removed and cells were irradiated at either 662 keV or 10 MV. At the 0.25 mg Fe/mL dose (4 pg Fe/cell), radiation energy did not affect the level of cytotoxicity. However with 1.0 mg Fe/mL (9 pg Fe/cell), the higher 10 MV radiation energy resulted in 50% greater cytotoxicity as compared to cells without IONPs irradiated at this energy. These results suggest IONPs may be able to significantly enhance the cytotoxic effects of radiation and improve therapeutic ratio if they can be selectively associated with cancer cells and/or tumors. Ongoing in vivo studies of IONP radiation enhancement in a murine tumor model are too immature to draw conclusions from at this time, however preliminary data suggests similar effectiveness of IONP radiation enhancement at 6 MV and 18 MV energy levels. In addition to the IONP-based radiation enhancement demonstrated here, the use of tumor-localized IONP with an externally delivered, non-toxic alternating magnetic field affords the opportunity to selectively heat and kill tumor cells. Combining IONP-based radiation sensitization and heat-based cytotoxicity provides a unique and potentially highly effective opportunity for therapeutic ratio enhancement.

Proceedings Article | 26 February 2013 Paper

Magnetic nanoparticle hyperthermia: predictive model for temperature distribution

Robert Stigliano, Fridon Shubitidze, Alicia Petryk, Jennifer Tate, P. Jack Hoopes

Proceedings Volume 8584, 858410 (2013) https://doi.org/10.1117/12.2007673

KEYWORDS: Magnetism, Tumors, Tissues, Nanoparticles, Thermal modeling, Data modeling, Temperature metrology, Cancer, Particles, In vivo imaging

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Proceedings Article | 26 February 2013 Paper

Imaging and modification of the tumor vascular barrier for improvement in magnetic nanoparticle uptake and hyperthermia treatment efficacy

P. Jack Hoopes, Alicia Petryk, Jennifer Tate, Mark Savellano, Rendall Strawbridge, Andrew Giustini, Radu Stan, Barjor Gimi, Michael Garwood

Proceedings Volume 8584, 858403 (2013) https://doi.org/10.1117/12.2008689

KEYWORDS: Tumors, Nanoparticles, Magnetism, Iron, Magnetic resonance imaging, Tissues, Magnesium, Computed tomography, X-ray computed tomography, Cancer

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The predicted success of nanoparticle based cancer therapy is due in part to the presence of the inherent leakiness of the tumor vascular barrier, the so called enhanced permeability and retention (EPR) effect. Although the EPR effect is present in varying degrees in many tumors, it has not resulted in the consistent level of nanoparticle-tumor uptake enhancement that was initially predicted. Magnetic/iron oxide nanoparticles (mNPs) have many positive qualities, including their inert/nontoxic nature, the ability to be produced in various sizes, the ability to be activated by a deeply penetrating and nontoxic magnetic field resulting in cell-specific cytotoxic heating, and the ability to be successfully coated with a wide variety of functional coatings. However, at this time, the delivery of adequate numbers of nanoparticles to the tumor site via systemic administration remains challenging. Ionizing radiation, cisplatinum chemotherapy, external static magnetic fields and vascular disrupting agents are being used to modify the tumor environment/vasculature barrier to improve mNP uptake in tumors and subsequently tumor treatment. Preliminary studies suggest use of these modalities, individually, can result in mNP uptake improvements in the 3-10 fold range. Ongoing studies show promise of even greater tumor uptake enhancement when these methods are combined. The level and location of mNP/Fe in blood and normal/tumor tissue is assessed via histopathological methods (confocal, light and electron microscopy, histochemical iron staining, fluorescent labeling, TEM) and ICP-MS. In order to accurately plan and assess mNP-based therapies in clinical patients, a noninvasive and quantitative imaging technique for the assessment of mNP uptake and biodistribution will be necessary. To address this issue, we examined the use of computed tomography (CT), magnetic resonance imaging (MRI), and Sweep Imaging With Fourier Transformation (SWIFT), an MRI technique which provides a positive iron contrast enhancement and a reduced signal to noise ratio, for effective observation and quantification of Fe/mNP concentrations in the clinical setting.

Proceedings Article | 26 February 2013 Paper

Improved delivery of magnetic nanoparticles with chemotherapy cancer treatment

Alicia Petryk, Andrew Giustini, Rachel Gottesman, P. Jack Hoopes

Proceedings Volume 8584, 85840H (2013) https://doi.org/10.1117/12.2007308

KEYWORDS: Tumors, Iron, Tissues, Nanoparticles, Cancer, Oncology, Magnesium, Magnetism, Liver, Spleen

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Proceedings Article | 26 February 2013 Paper

Development of a biodegradable iron oxide nanoparticle gel for tumor bed therapy

B. Cunkelman, E. Chen, A. Petryk, J. Tate, S. Thappa, R. Collier, P. Hoopes

Proceedings Volume 8584, 858411 (2013) https://doi.org/10.1117/12.2007310

KEYWORDS: Tumors, Cancer, Nanoparticles, Magnetism, Oxides, Oncology, Iron, Tissues, In vivo imaging, Polymer thin films

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Proceedings Article | 14 April 2012 Paper

In vivo imaging and quantification of iron oxide nanoparticle uptake and biodistribution

P. Jacks Hoopes, Alicia Petryk, Barjor Gimi, Andrew Giustini, John Weaver, John Bischof, Ryan Chamberlain, Michael Garwood

Proceedings Volume 8317, 83170R (2012) https://doi.org/10.1117/12.916097

KEYWORDS: Tumors, Magnetic resonance imaging, Iron, Tissues, Computed tomography, In vivo imaging, Nanoparticles, Oxides, Magnetism, Cancer

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Proceedings Article | 24 February 2011 Paper

Nanoparticle-based cancer treatment: can delivered dose and biological dose be reliably modeled and quantified?

P. Jack Hoopes, Alicia Petryk, Andrew Giustini, Robert Stigliano, Robert D'Angelo, Jennifer Tate, Shiraz Cassim, Allan Foreman, John Bischof, John Pearce, Thomas Ryan

Proceedings Volume 7901, 79010A (2011) https://doi.org/10.1117/12.877026

KEYWORDS: Tumors, Tissues, Nanoparticles, Cancer, Algorithm development, Magnetism, Thermal effects, Microwave radiation, 3D modeling, Temperature metrology

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Essential developments in the reliable and effective use of heat in medicine include: 1) the ability to model energy deposition and the resulting thermal distribution and tissue damage (Arrhenius models) over time in 3D, 2) the development of non-invasive thermometry and imaging for tissue damage monitoring, and 3) the development of clinically relevant algorithms for accurate prediction of the biological effect resulting from a delivered thermal dose in mammalian cells, tissues, and organs. The accuracy and usefulness of this information varies with the type of thermal treatment, sensitivity and accuracy of tissue assessment, and volume, shape, and heterogeneity of the tumor target and normal tissue. That said, without the development of an algorithm that has allowed the comparison and prediction of the effects of hyperthermia in a wide variety of tumor and normal tissues and settings (cumulative equivalent minutes/ CEM), hyperthermia would never have achieved clinical relevance. A new hyperthermia technology, magnetic nanoparticle-based hyperthermia (mNPH), has distinct advantages over the previous techniques: the ability to target the heat to individual cancer cells (with a nontoxic nanoparticle), and to excite the nanoparticles noninvasively with a noninjurious magnetic field, thus sparing associated normal cells and greatly improving the therapeutic ratio. As such, this modality has great potential as a primary and adjuvant cancer therapy. Although the targeted and safe nature of the noninvasive external activation (hysteretic heating) are a tremendous asset, the large number of therapy based variables and the lack of an accurate and useful method for predicting, assessing and quantifying mNP dose and treatment effect is a major obstacle to moving the technology into routine clinical practice. Among other parameters, mNPH will require the accurate determination of specific nanoparticle heating capability, the total nanoparticle content and biodistribution in the target cells/tissue, and an effective and matching alternating magnetic field (AMF) for optimal and safe excitation of the nanoparticles. Our initial studies have shown that appropriately delivered and targeted nanoparticles are capable of achieving effective tumor cytotoxicity at measured thermal doses significantly less than the understood thermal dose values necessary to achieve equivalent treatment effects using conventional heat delivery techniques. Therefore conventional CEM based thermal dose - tissues effect relationships will not hold for mNPH. The goal of this effort is to provide a platform for determining the biological and physical parameters that will be necessary for accurately planning and performing safe and effective mNPH, creating a new, viable primary or adjuvant cancer therapy.

Proceedings Article | 23 February 2011 Paper

In vivo biodistribution of iron oxide nanoparticles: an overview

Jennifer Tate, Alicia Petryk, Andrew Giustini, P. Jack Hoopes

Proceedings Volume 7901, 790117 (2011) https://doi.org/10.1117/12.876414

KEYWORDS: Nanoparticles, Iron, Tissues, Tumors, Oxides, Liver, Magnesium, Particles, Spleen, Kidney

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Proceedings Article | 23 February 2011 Paper

Comparison of iron oxide nanoparticle and microwave hyperthermia alone or combined with cisplatinum in murine breast tumors

Alicia Petryk, Robert Stigliano, Andrew Giustini, Rachel Gottesman, B. Trembly, Peter Kaufman, P. Jack Hoopes

Proceedings Volume 7901, 790119 (2011) https://doi.org/10.1117/12.876535

KEYWORDS: Tumors, Microwave radiation, Nanoparticles, Iron, Oxides, Therapeutics, Tissues, Cancer, Radiation effects, Magnetism

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Proceedings Article | 23 February 2011 Paper

Comparison of microwave and magnetic nanoparticle hyperthermia radiosensitization in murine breast tumors

Andrew Giustini, Alicia Petryk, Paul Hoopes

Proceedings Volume 7901, 79010E (2011) https://doi.org/10.1117/12.876515

KEYWORDS: Tumors, Nanoparticles, Microwave radiation, Magnetism, Iron, Tissues, Cancer, Breast, Magnesium, Heat treatments

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Proceedings Article | 23 February 2011 Paper

Kinetics and pathogenesis of intracellular magnetic nanoparticle cytotoxicity

Andrew Giustini, Rachel Gottesman, A. Petryk, A. Rauwerdink, P. Jack Hoopes

Proceedings Volume 7901, 790118 (2011) https://doi.org/10.1117/12.876519

KEYWORDS: Nanoparticles, Synthetic aperture radar, Magnetism, Tumors, Transmission electron microscopy, Iron, Pathogens, Oxides, Tissues, Toxicity

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Proceedings Article | 24 February 2009 Paper

Iron oxide nanoparticle hyperthermia and chemotherapy cancer treatment

A. Petryk, A. Giustini, P. Ryan, R. Strawbridge, P. Hoopes

Proceedings Volume 7181, 71810N (2009) https://doi.org/10.1117/12.810024

KEYWORDS: Tumors, Nanoparticles, Iron, Oxides, Cancer, In vivo imaging, In vitro testing, Oncology, Magnesium, Microelectromechanical systems

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Proceedings Article | 24 February 2009 Paper

Assessment of intratumor non-antibody directed iron oxide nanoparticle hyperthermia cancer therapy and antibody directed IONP uptake in murine and human cells

P. Hoopes, J. Tate, J. Ogden, R. Strawbridge, S. Fiering, A. Petryk, S. Cassim, A. Giustini, E. Demidenko, R. Ivkov, S. Barry, P. Chinn, A. Foreman

Proceedings Volume 7181, 71810P (2009) https://doi.org/10.1117/12.812056

KEYWORDS: Tumors, Nanoparticles, Iron, Particles, Oxides, Cancer, In vivo imaging, In vitro testing, Tissues, Oncology

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Hyperthermia, as an independent modality or in combination with standard cancer treatments such as chemotherapy and radiation, has been established in vitro and in vivo as an effective cancer treatment. However, despite efforts over the past 25 years, such therapies have never been optimized or widelyaccepted clinically. Although methods continue to improve, conventionally-delivered heat (RF, ultrasound, microwave etc) can not be delivered in a tumor selective manner. The development of antibody-targeted, or even nontargeted, biocompatible iron oxide nanoparticles (IONP) now allows delivery of cytotoxic heat to individual cancer cells. Using a murine mouse mammary adenocarcinoma (MTGB) and human colon carcinoma (HT29) cells, we studied the biology and treatment of IONP hyperthermia tumor treatment. Methods: Cancer cells (1 x 10⁶) with or without iron oxide nanoparticles (IONP) were studied in culture or in vivo via implanted subcutaneously in female C3H mice, Tumors were grown to a treatment size of 150 mm³ and tumors volumes were measured using standard 3-D caliper measurement techniques. Mouse tumors were heated via delivery of an alternating magnetic field, which activated the nanoparticles, using a cooled 36 mm diameter square copper tube induction coil which provided optimal heating in 1.5 cm wide region of the coil. The IONPs were dextran coated and had a hydrodynamic radius of approximately 100 nm. For the in vivo studies, intra-tumor, peritumor and rectal (core body) temperatures were continually measured throughout the treatment period. Results: Although some eddy current heating was generated in non-target tissues at the higher field strengths, our preliminary IONP hyperthermia studies show that whole mouse AMF exposure @160 KHz and 400 or 550 Oe, for a 20 minutes (heat-up and protocol heating), provides a safe and efficacious tumor treatment. Initial electron and light microscopic studies (in vitro and in vivo) showed the 100 nm used in our studies are rapidly taken up and retained by the tumor cells. Additional in vitro studies suggest antibodies can significantly enhance the cellular uptake of IONPs.

Proceedings Article | 24 February 2009 Paper

Iron oxide nanoparticle hyperthermia and radiation cancer treatment

S. Cassim, A. Giustini, A. Petryk, R. Strawbridge, P. Hoopes

Proceedings Volume 7181, 71810O (2009) https://doi.org/10.1117/12.810035

KEYWORDS: Tumors, Nanoparticles, Iron, Oxides, Radiotherapy, Cancer, Radiation effects, Tissues, Oncology, Magnetism

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It is established that heat can enhance the effect of radiation cancer treatment. Due to the ability to localize thermal energy using nanoparticle hyperthermia, as opposed to other, less targeted, hyperthermia modalities, it appears such enhancement could be accomplished without complications normally associated with systemic or regional hyperthermia. This study employs non-curative (suboptimal), doses of heat and radiation, in an effort to determine the therapeutic enhancement potential for IONP hyperthermia and radiation. Methods: MTG-B murine breast adenocarcinoma cell are inoculated into the right flanks of female CH3/HEJ mice and grown to volumes of 150mm³⁺ /_- 40 mm³. A single dose of 15 Gy (6 MeV) radiation was uniformly delivered to the tumor. A pre-defined thermal dose is delivered by direct injection of iron oxide nanoparticles into the tumor. By adjusting the field strength of the 160 KHz alternating magnetic field (AMF) an intra-tumoral temperature between 41.5 and 43 degrees Celsius was maintained for 10min. The alternating magnetic field was delivered by a water-cooled 36mm diameter square copper tube induction coil operating at 160 kHz with variable magnet field strengths up to 450 Oe . The primary endpoint of the study is the number of days required for the tumor to achieve a volume 3 fold greater than the volume at the time of treatment (tumor regrowth delay). Results: Preliminary results suggest the addition of a modest IONP hyperthermia to 15 Gy radiation achieved an approximate 50% increase in tumor regrowth delay as compared to a 15 Gy radiation treatment alone. The therapeutic effects of IONP heat and radiation combined were considered additive, however in mice that demonstrated complete response (no tumor present after 30 days), the effect was considered superadditive or synergistic. Although this data is very encouraging from a multimodality cancer therapy standpoint, additional temporal and dose related information is clearly necessary to optimize the therapy.

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