In this paper by the methods of Raman spectroscopy, a drop of the evaporating liquid of Human Serum Albumin with the Rhodamine 6G dye molecules in the presence of silver nanoparticles obtained by laser femtosecond ablation in water was studied. In the Raman spectra, an increase in the intensity of scattering of dye molecules with silver nanoparticles the addition of protein molecules was recorded. We suppose that the observed effect is associated with the diffusion and thermal processes in the drop arising during laser excitation.
In this paper we perform results of conformational analysis of septic human serum albumin (HSA) carried out by Raman spectroscopy (RS), infrared (IR) spectroscopy and fluorescent spectroscopy. The main vibrational groups were identified and analyzed for septic HSA and its health control. Comparison between Raman and IR results were done. Fluorescent spectral changes of Trp-214 group were analyzed. Application of Raman, IR spectroscopy, fluorescent spectroscopy for conformational changes study of HSA during pathology were shown.
The study of the luminescence of CdZnSeS / ZnS quantum dots (QDs) absorbed on the rough surface of a silver film, including the energy transfer between human serum albumin molecules, isolated from the blood plasma of healthy and infected with sepsis patients, was performed by spectral-kinetic methods.
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