Dr. Leonardo Sacconi Profile

Dr. Leonardo Sacconi

Researcher at LENS - Lab Europeo di Spettroscopie Non-Lineari

SPIE Involvement:

Author

Publications (45)

Proceedings Article | 1 April 2020 Presentation + Paper

Presentation + Paper

Two-photon light-sheet microscopy for high-speed whole-brain functional imaging of zebrafish neuronal physiology and pathology

Giuseppe de Vito, Lapo Turrini, Chiara Fornetto, Pietro Ricci, Caroline Müllenbroich, Giuseppe Sancataldo, Elena Trabalzini, Giacomo Mazzamuto, Natascia Tiso, Leonardo Sacconi, Duccio Fanelli, Ludovico Silvestri, Francesco Vanzi, Francesco Saverio Pavone

Proceedings Volume 11360, 1136004 (2020) https://doi.org/10.1117/12.2560341

KEYWORDS: Brain, Microscopy, Physiology, Pathology, Microscopes, Polarization, Visualization, Calcium, Functional imaging, Epilepsy

Read Abstract +

Proceedings Article | 21 February 2020 Paper

Paper

Two-photon high-speed light-sheet volumetric imaging of brain activity during sleep in zebrafish larvae

Giuseppe de Vito, Chiara Fornetto, Pietro Ricci, Caroline Müllenbroich, Giuseppe Sancataldo, Lapo Turrini, Giacomo Mazzamuto, Natascia Tiso, Leonardo Sacconi, Duccio Fanelli, Ludovico Silvestri, Francesco Vanzi, Francesco Saverio Pavone

Proceedings Volume 11226, 1122604 (2020) https://doi.org/10.1117/12.2542285

KEYWORDS: Brain, Calcium, Neuroimaging, Microscopes, Microscopy

Read Abstract +

Proceedings Article | 22 July 2019 Paper

Paper

Three-dimensional analysis of human brain cytoarchitectonics by means of a SWITCH/TDE-combined clearing method

Irene Costantini, Giacomo Mazzamuto, Annunziatina Laurino, Erica Lazzeri, Leonardo Sacconi, Mattia Neri, Andrea Simonetto, Matteo Roffilli, Ludovico Silvestri, Francesco S. Pavone

Proceedings Volume 11076, 110760B (2019) https://doi.org/10.1117/12.2527096

KEYWORDS: Brain, Tissues, Analytical research, Neuroimaging, Tissue optics, Image resolution, Biological research

Read Abstract +

Proceedings Article | 14 March 2018 Presentation

Presentation

Real-time optical manipulation of the cardiac conduction pathway (Conference Presentation)

Emilia Margoni, Samantha Cannazzaro, Cecilia Ferrantini, Raffaele Coppini, Pin Yan, Leslie Loew, Leonardo Bocchi, Marina Campione, Elisabetta Cerbai, Corrado Poggesi, Gil Bub, Franceaco Pavone, Leonardo Sacconi

Proceedings Volume 10482, 104820E (2018) https://doi.org/10.1117/12.2288467

KEYWORDS: Optogenetics, Software development, Heart, Optical manipulation, Electrophysiology, Ultrafast phenomena, Micromirrors, Temporal resolution, Wave propagation

Read Abstract +

Proceedings Article | 14 March 2018 Presentation

Presentation

Whole heart cytoarchitecture at micron-scale resolution (Conference Presentation)

Erica Lazzeri, Irene Costantini, Cecilia Ferrantini, Samantha Cannazzaro, Giacomo Mazzamuto, Claudia Crocini, Raffaele Coppini, Leonardo Bocchi, Elisabetta Cerbai, Corrado Poggesi, Francesco Pavone, Leonardo Sacconi

Proceedings Volume 10471, 104710L (2018) https://doi.org/10.1117/12.2288553

KEYWORDS: Heart, Collagen, 3D modeling, Tissues, Associative arrays, Genetics, Tissue clearing, Optical microscopy, Second-harmonic generation, Action potentials

Read Abstract +

SPIE Journal Paper | 19 February 2018 Open Access

Open Access

Confocal multispot microscope for fast and deep imaging in semicleared tissues

Marie-Pierre Adam, Marie Caroline Müllenbroich, Antonino Paolo Di Giovanna, Domenico Alfieri, Ludovico Silvestri, Leonardo Sacconi, Francesco Saverio Pavone

JBO, Vol. 23, Issue 02, 020503, (February 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.2.020503

KEYWORDS: Microscopes, Camera shutters, Tissues, Brain, Confocal microscopy, Point spread functions, Scattering, Cameras, Neuroimaging, Diffractive optical elements

Read Abstract +

Proceedings Article | 28 July 2017 Paper

Paper

Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy

Antonino Paolo Di Giovanna, Alessandro Tibo, Ludovico Silvestri, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, Francesco Saverio Pavone

Proceedings Volume 10414, 104140N (2017) https://doi.org/10.1117/12.2285044

KEYWORDS: Vascular imaging, Neuroimaging, Brain, Microscopy, Tissue clearing

Read Abstract +

Proceedings Article | 19 April 2017 Presentation

Presentation

Rehabilitation-triggered cortical plasticity after stroke: in vivo imaging at multiple scales (Conference Presentation)

Anna Letizia Allegra Mascaro, Emilia Conti, Stefano Lai, Cristina Spalletti, Antonino Paolo Di Giovanna, Claudia Alia, Alessandro Panarese, Leonardo Sacconi, Silvestro Micera, Matteo Caleo, Francesco Pavone

Proceedings Volume 10051, 100510L (2017) https://doi.org/10.1117/12.2250860

KEYWORDS: Robotics, In vivo imaging, Brain, Two photon excitation microscopy, Spine, Neurons, Cortical activation, Optogenetics, Functional imaging, Current controlled current source

Read Abstract +

Proceedings Article | 19 April 2017 Presentation

Presentation

High-fidelity functional and structural whole-brain imaging with Bessel-beam light-sheet microscopy (Conference Presentation)

Marie Caroline Müllenbroich, Ludovico Silvestri, Lapo Turrini, Antonino Paolo Di Giovanna, Tommaso Alterini, Ali Gheisari, Pietro Ricci, Leonardo Sacconi, Francesco Vanzi, Francesco Pavone

Proceedings Volume 10051, 100510F (2017) https://doi.org/10.1117/12.2251471

KEYWORDS: Optical resolution, Calcium, Microscopy, Structural imaging, Brain, Tissue clearing, Blood, Neuroscience, Neuroimaging, Image resolution

Read Abstract +

Proceedings Article | 27 April 2016 Presentation

Presentation

Label-free NIR reflectance imaging as a complimentary tool for two-photon fluorescence microscopy: multimodal investigation of stroke (Conference Presentation)

Anna Letizia Allegra Mascaro, Irene Costantini, Emilia Margoni, Giulio Iannello, Alessandro Bria, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9712, 97121K (2016) https://doi.org/10.1117/12.2208886

KEYWORDS: Near infrared, Reflectivity, Brain, Two photon excitation microscopy, Two photon imaging, Photons, Axons, Blood circulation, Luminescence, Imaging spectroscopy

Read Abstract +

Proceedings Article | 26 April 2016 Presentation

Presentation

Optogenetic control of the cardiac conduction system (Conference Presentation)

Claudia Crocini, Cecilia Ferrantini, Raffaele Coppini, Leslie Loew, Elisabetta Cerbai, Corrado Poggesi, Francesco Pavone, Leonardo Sacconi

Proceedings Volume 9690, 96902E (2016) https://doi.org/10.1117/12.2208280

KEYWORDS: Heart, Optogenetics, Control systems, Spectroscopy, Mouse models, Channel projecting optics, Action potentials, Voltage sensitive dyes, Cameras, Imaging systems

Read Abstract +

Proceedings Article | 26 April 2016 Presentation

Presentation

Mapping whole-brain activity with cellular resolution by light-sheet microscopy and high-throughput image analysis (Conference Presentation)

Ludovico Silvestri, Nikita Rudinskiy, Marco Paciscopi, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, Bradley Hyman, Francesco Pavone

Proceedings Volume 9690, 969012 (2016) https://doi.org/10.1117/12.2208603

KEYWORDS: Brain, Brain mapping, Image resolution, Microscopy, Image analysis, Image processing, Neurons, Imaging spectroscopy, Spectroscopy, Neuroimaging

Read Abstract +

SPIE Journal Paper | 28 March 2016 Open Access

Open Access

Clearing of fixed tissue: a review from a microscopist’s perspective

Ludovico Silvestri, Irene Costantini, Leonardo Sacconi, Francesco Saverio Pavone

JBO, Vol. 21, Issue 08, 081205, (March 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.8.081205

KEYWORDS: Tissues, Tissue optics, Luminescence, Brain, Refractive index, Microscopy, Transparency, Confocal microscopy, Taxonomy, Proteins

Read Abstract +

Proceedings Article | 14 July 2015 Paper

Paper

A versatile new technique to clear mouse and human brain

Irene Costantini, Antonino Paolo Di Giovanna, Anna Letizia Allegra Mascaro, Ludovico Silvestri, Marie Caroline Müllenbroich, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9536, 95360X (2015) https://doi.org/10.1117/12.2184289

KEYWORDS: Brain, Tissues, Refractive index, Luminescence, Neuroimaging, Optical clearing, Microscopy, Transparency, Tomography, Image resolution

Read Abstract +

SPIE Journal Paper | 29 June 2015 Open Access

Open Access

Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains

M. Caroline Müllenbroich, Ludovico Silvestri, Leonardo Onofri, Irene Costantini, Marcel van’t Hoff, Leonardo Sacconi, Giulio Iannello, Francesco S. Pavone

NPh, Vol. 2, Issue 04, 041404, (June 2015) https://doi.org/10.1117/12.10.1117/1.NPh.2.4.041404

KEYWORDS: Brain, Microscopes, Cameras, Objectives, Neuroimaging, Mirrors, Microscopy, Confocal microscopy, Data acquisition, Camera shutters

Read Abstract +

Proceedings Article | 10 March 2015 Paper

Paper

A new versatile clearing method for brain imaging

Irene Costantini, Antonino Paolo Di Giovanna, Anna Letizia Allegra Mascaro, Ludovico Silvestri, Marie Caroline Müllenbroich, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9305, 930514 (2015) https://doi.org/10.1117/12.2076908

KEYWORDS: Brain, Tissues, Refractive index, Luminescence, Neuroimaging, Tissue optics, Optical clearing, Tomography, Microscopy, Brain imaging

Read Abstract +

Proceedings Article | 10 March 2015 Paper

Paper

Whole brain optical imaging

Ludovico Silvestri, Anna Letizia Allegra Mascaro, Irene Costantini, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 9305, 93051O (2015) https://doi.org/10.1117/12.2087339

KEYWORDS: Microscopy, Brain, Neuroimaging, Neurons, In vivo imaging, Electron microscopy, Axons, Optical imaging, Neurophotonics, Visualization

Read Abstract +

SPIE Journal Paper | 6 March 2015 Open Access

Open Access

Towards a comprehensive understanding of brain machinery by correlative microscopy

Anna Letizia Allegra Mascaro, Ludovico Silvestri, Leonardo Sacconi, Francesco Pavone

JBO, Vol. 20, Issue 06, 061105, (March 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.6.061105

KEYWORDS: Brain, Microscopy, Neuroimaging, In vivo imaging, Functional magnetic resonance imaging, Neurons, Axons, Calcium, Electron microscopy, Brain mapping

Read Abstract +

Proceedings Article | 5 March 2015 Open Access

Open Access

Paper

Multiphoton microscopy in brain imaging

A. L. Allegra Mascaro, L. Silvestri, I. Costantini, L. Sacconi, B. Maco, G. Knott, F. Pavone

Proceedings Volume 9329, 932903 (2015) https://doi.org/10.1117/12.2084183

KEYWORDS: Neurons, Brain, Microscopy, In vivo imaging, Multiphoton microscopy, Two photon excitation microscopy, Brain imaging, Neuroimaging, Tissues, Confocal microscopy

Read Abstract +

Proceedings Article | 8 May 2014 Paper

Paper

Simultaneous recording of t-tubular electrical activity and Ca2+-release in heart failure

C. Crocini, R. Coppini, C. Ferrantini, P. Yan, L. Loew, C. Tesi, C. Poggesi, E. Cerbai, F. Pavone, L. Sacconi

Proceedings Volume 9129, 91291I (2014) https://doi.org/10.1117/12.2053523

KEYWORDS: Heart, Microscopes, Electrical breakdown, Action potentials, Adaptive optics, Ultrafast phenomena, Two photon excitation microscopy, Voltage sensitive dyes, Glasses, Magnetism

Read Abstract +

Proceedings Article | 24 March 2014 Paper

Paper

In vivo imaging of neural reactive plasticity after laser axotomy in cerebellar cortex

A. L. Allegra Mascaro, L. Sacconi, B. Maco, G. Knott, F. Pavone

Proceedings Volume 8928, 89281N (2014) https://doi.org/10.1117/12.2037276

KEYWORDS: In vivo imaging, Axons, Neurons, Californium, Electron microscopy, Dendrites, Optical fibers, Cranial windows, Two photon imaging, Microscopy

Read Abstract +

Proceedings Article | 28 February 2014 Paper

Paper

Exploring the brain on multiple scales with correlative two-photon and light sheet microscopy

Ludovico Silvestri, Anna Letizia Allegra Mascaro, Irene Costantini, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 8948, 89480I (2014) https://doi.org/10.1117/12.2037867

KEYWORDS: Brain, Microscopy, Neurons, Blood vessels, In vivo imaging, Objectives, Neuroimaging, Two photon excitation microscopy, Confocal microscopy, Microscopes

Read Abstract +

Proceedings Article | 24 June 2013 Paper

Paper

Probing cell activity in random access modality

L. Sacconi, C. Crocini, J. Lotti, R. Coppini, C. Ferrantini, C. Tesi, P. Yan, L. Loew, E. Cerbai, C. Poggesi, F. Pavone

Proceedings Volume 8804, 880406 (2013) https://doi.org/10.1117/12.2030585

KEYWORDS: Microscopes, Voltage sensitive dyes, Neurons, Luminescence, Microscopy, Multiplexing, Temporal resolution, Signal detection, Action potentials, Optical recording

Read Abstract +

Proceedings Article | 24 June 2013 Paper

Paper

In vivo two-photon imaging of climbing fibers plasticity after laser axotomy

A. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, G. Mandolesi, B. Maco, G. Knott, V. De Paola, P. Strata, F. Pavone

Proceedings Volume 8804, 880407 (2013) https://doi.org/10.1117/12.2032180

KEYWORDS: Californium, In vivo imaging, Injuries, Two photon imaging, Optical fibers, Axons, Green fluorescent protein, Nervous system, Information operations, Luminescence

Read Abstract +

Proceedings Article | 24 June 2013 Paper

Paper

Light sheet microscopy of cleared mouse brains: aberrations effects caused by refractive index mismatch

Ludovico Silvestri, Leonardo Sacconi, Francesco Pavone

Proceedings Volume 8804, 880405 (2013) https://doi.org/10.1117/12.2032488

KEYWORDS: Microscopy, Monochromatic aberrations, Confocal microscopy, Refractive index, Point spread functions, Objectives, Brain, Luminescence, Image resolution, Neuroimaging

Read Abstract +

Proceedings Article | 17 June 2013 Paper

Paper

Neural plasticity explored by correlative two-photon and electron/SPIM microscopy

A. L. Allegra Mascaro, L. Silvestri, I. Costantini, L. Sacconi, B. Maco, G. Knott, F. Pavone

Proceedings Volume 8797, 87970G (2013) https://doi.org/10.1117/12.2032505

KEYWORDS: Microscopy, In vivo imaging, Neurons, Brain, Confocal microscopy, Neuroimaging, Electron microscopy, Axons, Two photon excitation microscopy, Luminescence

Read Abstract +

Proceedings Article | 22 February 2013 Paper

Paper

In vivo reactive neural plasticity investigation by means of correlative two photon: electron microscopy

A. L. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, G. Mandolesi, B. Maco, G. Knott, L. Huang, V. De Paola, P. Strata, F. S. Pavone

Proceedings Volume 8588, 85881A (2013) https://doi.org/10.1117/12.2005953

KEYWORDS: Californium, In vivo imaging, Skull, Axons, Injuries, Green fluorescent protein, Electron microscopy, Brain, Surgery, Nervous system

Read Abstract +

Proceedings Article | 22 February 2013 Paper

Paper

Probing the spatiotemporal relationship between intracellular Ca2+ release and action potential propagation in cardiomyocytes by ultrafast multi-photon random access microscopy

L. Sacconi, C. Crocini, R. Coppini, C. Ferrantini, C. Tesi, P. Yan, L. Loew, E. Cerbai, C. Poggesi, F. Pavone

Proceedings Volume 8588, 85881B (2013) https://doi.org/10.1117/12.2006109

KEYWORDS: Calcium, Action potentials, Neodymium, Ultrafast phenomena, Microscopes, Ions, Multiphoton microscopy, Microscopy, Heart, Microelectromechanical systems

Read Abstract +

SPIE Journal Paper | 18 June 2012 Open Access

Open Access

Protein conformation and molecular order probed by second-harmonic-generation microscopy

Francesco Vanzi, Leonardo Sacconi, Riccardo Cicchi, Francesco Pavone

JBO, Vol. 17, Issue 6, 060901, (June 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.6.060901

KEYWORDS: Second-harmonic generation, Proteins, Tissues, Microscopy, Molecules, Polarization, Collagen, Biomedical optics, Signal detection, Anisotropy

Read Abstract +

Proceedings Article | 3 March 2010 Paper

Paper

Plasticity of climbing fibers after laser axotomy

A. Allegra Mascaro, P. Cesare, L. Sacconi, G. Grasselli, P. Strata, F. Pavone

Proceedings Volume 7548, 754853 (2010) https://doi.org/10.1117/12.840378

KEYWORDS: Californium, In vivo imaging, Neurons, Injuries, Two photon imaging, Brain, Skull, Multiphoton processes, Neuroscience, Nervous system

Read Abstract +

Proceedings Article | 25 February 2010 Paper

Paper

Brain plasticity and functionality explored by nonlinear optical microscopy

L. Sacconi, L. Allegra, M. Buffelli, P. Cesare, E. D'Angelo, D. Gandolfi, G. Grasselli, J. Lotti, J. Mapelli, P. Strata, F. Pavone

Proceedings Volume 7589, 758907 (2010) https://doi.org/10.1117/12.847898

KEYWORDS: Second-harmonic generation, Neurons, Optical fibers, Microscopy, Microscopes, Californium, Brain, Signal attenuation, Optical recording, Tissue optics

Read Abstract +

In combination with fluorescent protein (XFP) expression techniques, two-photon microscopy has become an indispensable tool to image cortical plasticity in living mice. In parallel to its application in imaging, multi-photon absorption has also been used as a tool for the dissection of single neurites with submicrometric precision without causing any visible collateral damage to the surrounding neuronal structures. In this work, multi-photon nanosurgery is applied to dissect single climbing fibers expressing GFP in the cerebellar cortex. The morphological consequences are then characterized with time lapse 3-dimensional two-photon imaging over a period of minutes to days after the procedure. Preliminary investigations show that the laser induced fiber dissection recalls a regenerative process in the fiber itself over a period of days. These results show the possibility of this innovative technique to investigate regenerative processes in adult brain. In parallel with imaging and manipulation technique, non-linear microscopy offers the opportunity to optically record electrical activity in intact neuronal networks. In this work, we combined the advantages of second-harmonic generation (SHG) with a random access (RA) excitation scheme to realize a new microscope (RASH) capable of optically recording fast membrane potential events occurring in a wide-field of view. The RASH microscope, in combination with bulk loading of tissue with FM4-64 dye, was used to simultaneously record electrical activity from clusters of Purkinje cells in acute cerebellar slices. Complex spikes, both synchronous and asynchronous, were optically recorded simultaneously across a given population of neurons. Spontaneous electrical activity was also monitored simultaneously in pairs of neurons, where action potentials were recorded without averaging across trials. These results show the strength of this technique in describing the temporal dynamics of neuronal assemblies, opening promising perspectives in understanding the computations of neuronal networks.

Proceedings Article | 25 February 2009 Paper

Paper

Structural and molecular conformation of myosin in intact muscle fibers by second harmonic generation

V. Nucciotti, C. Stringari, L. Sacconi, F. Vanzi, M. Linari, G. Piazzesi, V. Lombardi, F. Pavone

Proceedings Volume 7183, 71831W (2009) https://doi.org/10.1117/12.809721

KEYWORDS: Second-harmonic generation, Polarization, Molecules, Head, Data modeling, Modulation, Harmonic generation, Proteins, Tissues, Optical fibers

Read Abstract +

Proceedings Article | 23 February 2009 Paper

Paper

Action potential detection by non-linear microscopy

Leonardo Sacconi, Jacopo Lotti, Rodney O’Connor, Jonathan Mapelli, Daniela Gandolfi, Egidio D'Angelo, Francesco Pavone

Proceedings Volume 7161, 71613F (2009) https://doi.org/10.1117/12.807966

KEYWORDS: Second-harmonic generation, Neurons, Microscopy, Microscopes, Signal attenuation, Optical recording, Action potentials, Electrodes, Brain, Bragg cells

Read Abstract +

Proceedings Article | 15 February 2008 Paper

Paper

Structural dynamics of the skeletal muscle fiber by second harmonic generation

V. Nucciotti, C. Stringari, L. Sacconi, F. Vanzi, M. Linari, G. Piazzesi, V. Lombardi, F. Pavone

Proceedings Volume 6860, 68600A (2008) https://doi.org/10.1117/12.762704

KEYWORDS: Second-harmonic generation, Polarization, Molecules, Data modeling, Head, Harmonic generation, Tissues, Proteins, Modulation, Structural dynamics

Read Abstract +

Proceedings Article | 15 February 2008 Paper

Paper

In vivo multi-photon nanosurgery on cortical neurons: focusing on network organization

L. Sacconi, R. O'Connor, A. Jasaitis, A. Masi, M. Buffelli, F. Pavone

Proceedings Volume 6860, 686024 (2008) https://doi.org/10.1117/12.761588

KEYWORDS: Dendrites, Spine, Neurons, In vivo imaging, Fluorescent proteins, Absorption, Two photon excitation microscopy, Two photon imaging, Green fluorescent protein, Brain

Read Abstract +

SPIE Journal Paper | 1 September 2007 Open Access

Open Access

In vivo multiphoton nanosurgery on cortical neurons

Leonardo Sacconi, Rod O'Connor, Audrius Jasaitis, Alessio Masi, Mario Buffelli, Francesco Pavone

JBO, Vol. 12, Issue 05, 050502, (September 2007) https://doi.org/10.1117/12.10.1117/1.2798723

KEYWORDS: Dendrites, Spine, Neurons, In vivo imaging, Fluorescent proteins, Two photon excitation microscopy, Absorption, Two photon imaging, Neuroscience, Multiphoton processes

Read Abstract +

Proceedings Article | 13 July 2007 Paper

Paper

In vivo micro-lesion of single dendrite with femtosecond laser pulses

L. Sacconi, A. Masi, G. Diana, M. Buffelli, F. Pavone

Proceedings Volume 6633, 663310 (2007) https://doi.org/10.1117/12.727874

KEYWORDS: Dendrites, Femtosecond phenomena, Two photon excitation microscopy, In vivo imaging, Spine, Luminescence, Microscopes, Neurons, Confocal microscopy, Brain

Read Abstract +

Proceedings Article | 12 July 2007 Paper

Paper

Functional imaging of skeletal muscle fiber in different physiological states by second harmonic generation

V. Nucciotti, C. Stringari, L. Sacconi, F. Vanzi, C. Tesi, N. Piroddi, C. Poggesi, C. Castiglioni, A. Milani, M. Linari, G. Piazzesi, V. Lombardi, F. Pavone

Proceedings Volume 6630, 663004 (2007) https://doi.org/10.1117/12.727962

KEYWORDS: Second-harmonic generation, Polarization, Molecules, Data modeling, Harmonic generation, Proteins, Tissues, Structured optical fibers, Mathematical modeling, Functional imaging

Read Abstract +

Proceedings Article | 10 February 2007 Paper

Paper

Study of skeletal muscle cross-bridge population dynamics by second harmonic generation

Valentina Nucciotti, C. Stringari, L. Sacconi, F. Vanzi, C. Tesi, N. Pirrodi, C. Poggesi, C. Castiglioni, A. Milani, M. Linari, G. Piazzesi, V. Lombardi, F. Pavone

Proceedings Volume 6442, 644219 (2007) https://doi.org/10.1117/12.700419

KEYWORDS: Second-harmonic generation, Polarization, Optical fibers, Data modeling, Harmonic generation, Tissues, Molecules, Proteins, Structural dynamics, Transducers

Read Abstract +

Proceedings Article | 10 February 2007 Paper

Paper

Laser-induced microlesion of single dendrites in living mice

L. Sacconi, R. Panteri, A. Masi, G. Diana, M. Buffelli, F. Keller, F. Pavone

Proceedings Volume 6442, 64421O (2007) https://doi.org/10.1117/12.700335

KEYWORDS: Dendrites, Spine, Two photon excitation microscopy, Brain, In vivo imaging, Neurons, Femtosecond phenomena, Luminescence, Microscopes, Pulsed laser operation

Read Abstract +

Proceedings Article | 23 February 2006 Paper

Paper

Functional imaging of muscle cells by second harmonic generation

Valentina Nucciotti, Leonardo Sacconi, Marco Linari, Vincenzo Lombardi, Gabriella Piazzesi, Nicoletta Piroddi, Corrado Poggesi, Chiara Tesi, Francesco Vanzi, Francesco Pavone

Proceedings Volume 6089, 60891I (2006) https://doi.org/10.1117/12.646004

KEYWORDS: Second-harmonic generation, Polarization, Harmonic generation, Scattering, Tissues, Proteins, Molecules, Functional imaging, Image resolution, Structural dynamics

Read Abstract +

Proceedings Article | 22 February 2006 Paper

Paper

Optical micromanipulation inside the cell: a focus in cell division

Leonardo Sacconi, Iva Tolic-Nørrelykke, Chiara Stringari, Francesco Pavone

Proceedings Volume 6088, 60881C (2006) https://doi.org/10.1117/12.644693

KEYWORDS: Spindles, Yeast, Microscopy, Proteins, Green fluorescent protein, Confocal microscopy, Optical tweezers, Optical micromanipulation, Multiphoton microscopy, Nonlinear optics

Read Abstract +

Proceedings Article | 29 March 2005 Paper

Paper

Laser nanosurgery and manipulation in living cells

Leonardo Sacconi, Iva Tolic-Norrelykke, Renzo Antolini, Francesco Pavone

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.585222

KEYWORDS: Spindles, Optical tweezers, Microscopes, Microscopy, Yeast, Optical manipulation, Data acquisition, Nonlinear optics, Cell biology, Mirrors

Read Abstract +

SPIE Journal Paper | 1 March 2005 Open Access

Open Access

Second-harmonic generation sensitivity to transmembrane potential in normal and tumor cells

Leonardo Sacconi, M. D'Amico, F. Vanzi, T. Biagiotti, Renzo Antolini, M. Olivotto, Francesco Pavone

JBO, Vol. 10, Issue 02, 024014, (March 2005) https://doi.org/10.1117/12.10.1117/1.1895205

KEYWORDS: Second-harmonic generation, Tumors, Polarization, Potassium, Electrophysiology, Luminescence, Molecules, Spectroscopy, Microscopes, Nonlinear optics

Read Abstract +

SPIE Journal Paper | 1 January 2005 Open Access

Open Access

Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear

Leonardo Sacconi, Iva Tolic-Nørrelykke, Renzo Antolini, Francesco Pavone

JBO, Vol. 10, Issue 01, 014002, (January 2005) https://doi.org/10.1117/12.10.1117/1.1854675

KEYWORDS: Spindles, Green fluorescent protein, 3D image processing, Microscopy, Yeast, Absorption, Image segmentation, Nonlinear optics, Microscopes, Femtosecond phenomena

Read Abstract +

Showing 5 of 45 publications

SIGN IN TO:

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Advertisement

Advertisement