Prof. Michael Börsch Profile

Prof. Michael Börsch

at Universitätsklinikum Jena

SPIE Involvement:

Conference Program Committee | Author

Publications (37)

Proceedings Article | 13 March 2024 Presentation

Monitoring ligand-dependent conformational dynamics of a single G-protein-coupled receptor by time-resolved FRET in an ABEL trap

Michael Börsch, Ivan Maslov, Valentin Borshchevskiy, Thomas Gensch

Proceedings Volume PC12847, PC128470W (2024) https://doi.org/10.1117/12.3005264

KEYWORDS: Fluorescence resonance energy transfer, Resonance energy transfer, Pulsed laser operation, Molecules, Molecular energy transfer, Confocal microscopy

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Proceedings Article | 12 March 2024 Presentation + Paper

Single NV center in nanodiamond for quantum sensing of protein dynamics in an ABEL trap

Iván Pérez, Anke Krueger, Jörg Wrachtrup, Fedor Jelezko, Michael Börsch

Proceedings Volume 12849, 1284906 (2024) https://doi.org/10.1117/12.3002280

KEYWORDS: Nanodiamonds, Fluorescence, Magnetism, Diffusion, Quantum sensing, Photon counting, Confocal microscopy, Single molecule spectroscopy

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Proceedings Article | 25 April 2023 Presentation + Paper

Monitoring oligomerization dynamics of individual human neurotensin receptors one in living cells and in SMALP nanodiscs

Lukas Spantzel, Iván Pérez, Thomas Heitkamp, Anika Westphal, Stefanie Reuter, Ralf Mrowka, Michael Börsch

Proceedings Volume 12384, 1238407 (2023) https://doi.org/10.1117/12.2648222

KEYWORDS: Confocal microscopy, Fluorescence, Fluorophores, Proteins, Microscopes, Fluorescence intensity, Fluorescence lifetime imaging, Copolymers

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Proceedings Article | 15 March 2023 Presentation

20 years of happy motoring with single FoF1-ATP synthase: exploring the speed limit (Conference Presentation)

Michael Börsch, Thomas Heitkamp, Iván Pérez

Proceedings Volume PC12386, PC1238602 (2023) https://doi.org/10.1117/12.2648056

KEYWORDS: Acquisition tracking and pointing, Fluorescence resonance energy transfer, Microscopes, Field programmable gate arrays, Feedback control, Electrodes, Control systems, Confocal microscopy

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Proceedings Article | 22 February 2019 Paper

Ligand-induced oligomerization of the human GPCR neurotensin receptor 1 monitored in living HEK293T cells

Anika Westphal, Hendrik Sielaff, Stefanie Reuter, Thomas Heitkamp, Ralf Mrowka, Michael Börsch

Proceedings Volume 10882, 108820W (2019) https://doi.org/10.1117/12.2506797

KEYWORDS: Receptors, Anisotropy, Luminescence, Fluorescence lifetime imaging, Microscopy, Confocal microscopy, Signal processing, Microscopes, Microfluidics

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Proceedings Article | 22 February 2019 Paper

Observing monomer: dimer transitions of neurotensin receptors 1 in single SMALPs by homoFRET and in an ABELtrap

André Dathe, Thomas Heitkamp, Iván Pérez, Hendrik Sielaff, Anika Westphal, Stefanie Reuter, Ralf Mrowka, Michael Börsch

Proceedings Volume 10884, 108840N (2019) https://doi.org/10.1117/12.2507546

KEYWORDS: Photons, Anisotropy, Receptors, Luminescence, Confocal microscopy, Diffusion, Proteins, Signal processing, Microscopy, Fluorescence correlation spectroscopy

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Proceedings Article | 23 February 2018 Paper

Towards monitoring conformational changes of the GPCR neurotensin receptor 1 by single-molecule FRET

Thomas Heitkamp, Reinhard Grisshammer, Michael Börsch

Proceedings Volume 10498, 104980T (2018) https://doi.org/10.1117/12.2286787

KEYWORDS: Anisotropy, Receptors, Fluorescence resonance energy transfer, Proteins, Luminescence, Photons, Confocal microscopy, Fluorescent proteins, Microscopes, Single molecule spectroscopy

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Proceedings Article | 20 February 2018 Paper

Analyzing conformational changes in single FRET-labeled A1 parts of archaeal A1AO-ATP synthase

Hendrik Sielaff, Dhirendra Singh, Gerhard Grüber, Michael Börsch

Proceedings Volume 10500, 1050007 (2018) https://doi.org/10.1117/12.2286785

KEYWORDS: Fluorescence resonance energy transfer, Photons, Luminescence, Acquisition tracking and pointing, Proteins, Molecules, Quantum efficiency, Quenching (fluorescence), Confocal microscopy, Energy transfer

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Proceedings Article | 27 February 2017 Paper

Confining Brownian motion of single nanoparticles in an ABELtrap

Maria Dienerowitz, Thomas Heitkamp, Thomas Gottschall, Jens Limpert, Michael Börsch

Proceedings Volume 10120, 1012017 (2017) https://doi.org/10.1117/12.2250550

KEYWORDS: Nanoparticles, Fluorescence resonance energy transfer, Diffusion, Proteins, Feedback control, Confocal microscopy, Particles, Electro optical systems, Single photon, Avalanche photodiodes, Microfluidics, Photons, Molecules, Laser scattering, Silver, Metals, Signal detection

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Proceedings Article | 21 February 2017 Paper

Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy

Franziska Foertsch, Mykhailo Ilchenko, Thomas Heitkamp, Silke Noßmann, Birgit Hoffmann, Ilka Starke, Ralf Mrowka, Christoph Biskup, Michael Börsch

Proceedings Volume 10071, 100710P (2017) https://doi.org/10.1117/12.2251210

KEYWORDS: Fluorescence lifetime imaging, Super resolution, Microscopy, Fluorescence resonance energy transfer, Resonance energy transfer, Live cell imaging, Anisotropy, Super resolution microscopy, Microscopes, Acquisition tracking and pointing, Proteins, Fluorescence anisotropy, Confocal microscopy

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Proceedings Article | 14 March 2016 Paper

Binding of the immunomodulatory drug Bz-423 to mitochondrial FoF1-ATP synthase in living cells by FRET acceptor photobleaching

Ilka Starke, Kathryn Johnson, Jan Petersen, Peter Gräber, Anthony Opipari, Gary Glick, Michael Börsch

Proceedings Volume 9712, 97120P (2016) https://doi.org/10.1117/12.2209645

KEYWORDS: Molecules, Modulation, Fluorescent proteins, Yeast, Confocal microscopy, Luminescence, Super resolution, Microscopes, Multiphoton microscopy, Microscopy, Glasses, Electron multiplying charge coupled devices

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Proceedings Article | 1 March 2016 Paper

Optimized green fluorescent protein fused to FoF1-ATP synthase for single-molecule FRET using a fast anti-Brownian electrokinetic trap

Maria Dienerowitz, Mykhailo Ilchenko, Bertram Su, Gabriele Deckers-Hebestreit, Günter Mayer, Thomas Henkel, Thomas Heitkamp, Michael Börsch

Proceedings Volume 9714, 971402 (2016) https://doi.org/10.1117/12.2209592

KEYWORDS: Fluorescence resonance energy transfer, Microfluidics, Imaging spectroscopy, Nanoparticles, Resonance energy transfer, Green fluorescent protein, Molecules, Luminescence, Confocal microscopy, Electro optics, Photon counting, Time correlated photon counting, Acquisition tracking and pointing, Microscopes, Diffusion

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Proceedings Article | 1 March 2016 Presentation + Paper

Observing single FoF1-ATP synthase at work using an improved fluorescent protein mNeonGreen as FRET donor

Thomas Heitkamp, Gabriele Deckers-Hebestreit, Michael Börsch

Proceedings Volume 9714, 97140B (2016) https://doi.org/10.1117/12.2209123

KEYWORDS: Fluorescence resonance energy transfer, Imaging spectroscopy, Acquisition tracking and pointing, Fluorescence lifetime imaging, Fluorescent proteins, Single molecule spectroscopy, Bacteria, In vitro testing, Catalysis, Switches, Proteins, Chromatography, Luminescence, Absorbance, Liquids, Computer programming, Fusion energy

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Proceedings Article | 9 March 2015 Paper

3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria

Anja Renz, Marc Renz, Diana Klütsch, Gabriele Deckers-Hebestreit, Michael Börsch

Proceedings Volume 9331, 93310D (2015) https://doi.org/10.1117/12.2080981

KEYWORDS: Diffusion, Acquisition tracking and pointing, Proteins, Microscopy, Glasses, Bacteria, Electron multiplying charge coupled devices, Calibration, Fluorescent proteins, Microscopes

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Proceedings Article | 5 March 2015 Paper

Observing conformations of single FoF1-ATP synthases in a fast anti-Brownian electrokinetic trap

Bertram Su, Monika Düser, Nawid Zarrabi, Thomas Heitkamp, Ilka Starke, Michael Börsch

Proceedings Volume 9329, 93290A (2015) https://doi.org/10.1117/12.2080975

KEYWORDS: Fluorescence resonance energy transfer, Microfluidics, Luminescence, Glasses, Molecules, Proteins, Field programmable gate arrays, Nanoparticles, Acquisition tracking and pointing, Quartz

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Proceedings Article | 1 April 2014 Paper

The regulatory switch of F1-ATPase studied by single-molecule FRET in the ABEL trap

Samuel Bockenhauer, Thomas Duncan, W. Moerner, Michael Börsch

Proceedings Volume 8950, 89500H (2014) https://doi.org/10.1117/12.2042688

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Luminescence, Proteins, Switches, Photons, Confocal microscopy, Field programmable gate arrays, Fluorescence correlation spectroscopy

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Proceedings Article | 28 February 2014 Paper

Regulatory conformational changes of the epsilon subunit in single FRET-labeled FoF1-ATP synthase

Thomas Duncan, Monika Düser, Thomas Heitkamp, Duncan G. McMillan, Michael Börsch

Proceedings Volume 8948, 89481J (2014) https://doi.org/10.1117/12.2040463

KEYWORDS: Fluorescence resonance energy transfer, Photons, Acquisition tracking and pointing, Proteins, Luminescence, Confocal microscopy, Liquids, Microscopes, X-rays, Chromatography

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Proceedings Article | 22 February 2013 Paper

Monitoring subunit rotation in single FRET-labeled FoF1-ATP synthase in an anti-Brownian electrokinetic trap

Thomas Heitkamp, Hendrik Sielaff, Anja Korn, Marc Renz, Nawid Zarrabi, Michael Börsch

Proceedings Volume 8588, 85880Q (2013) https://doi.org/10.1117/12.2002966

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Particles, Proteins, Photons, Monte Carlo methods, Confocal microscopy, Ion exchange, Luminescence, Optical simulations

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Proceedings Article | 22 February 2013 Paper

Manipulating freely diffusing single 20-nm particles in an Anti-Brownian Electrokinetic Trap (ABELtrap)

Nawid Zarrabi, Caterina Clausen, Monika Düser, Michael Börsch

Proceedings Volume 8587, 85870L (2013) https://doi.org/10.1117/12.2002952

KEYWORDS: Confocal microscopy, Field programmable gate arrays, Particles, Proteins, Fluorescence resonance energy transfer, Electrodes, Photons, Luminescence, Microwave radiation, Acquisition tracking and pointing

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Proceedings Article | 22 February 2013 Paper

Subunit rotation in single FRET-labeled F1-ATPase hold in solution by an anti-Brownian electrokinetic trap

Hendrik Sielaff, Thomas Heitkamp, Andrea Zappe, Nawid Zarrabi, Michael Börsch

Proceedings Volume 8590, 859008 (2013) https://doi.org/10.1117/12.2002955

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Monte Carlo methods, Proteins, Particles, Confocal microscopy, Data modeling, Photon counting, Chromatography, Time correlated photon counting

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Proceedings Article | 13 February 2012 Paper

Step size of the rotary proton motor in single FoF1-ATP synthase from a thermoalkaliphilic bacterium by DCO-ALEX FRET

Eva Hammann, Andrea Zappe, Stefanie Keis, Stefan Ernst, Doreen Matthies, Thomas Meier, Gregory Cook, Michael Börsch

Proceedings Volume 8228, 82280A (2012) https://doi.org/10.1117/12.907242

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Proteins, Photons, Anisotropy, Microscopes, Luminescence, Confocal microscopy, Temperature metrology, Fluorescence anisotropy

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Proceedings Article | 9 February 2012 Paper

Monitoring transient elastic energy storage within the rotary motors of single FoF1-ATP synthase by DCO-ALEX FRET

Stefan Ernst, Monika Düser, Nawid Zarrabi, Michael Börsch

Proceedings Volume 8226, 82260I (2012) https://doi.org/10.1117/12.907086

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Monte Carlo methods, Confocal microscopy, Proteins, Pulsed laser operation, Luminescence, Microscopes, Single photon, Beam splitters

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Proceedings Article | 9 February 2012 Paper

Diffusion properties of single FoF1-ATP synthases in a living bacterium unraveled by localization microscopy

Marc Renz, Torsten Rendler, Michael Börsch

Proceedings Volume 8225, 822513 (2012) https://doi.org/10.1117/12.907089

KEYWORDS: Diffusion, Proteins, Microscopy, Bacteria, Plasma, Luminescence, Acquisition tracking and pointing, Cameras, Objectives, Computer simulations

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SPIE Journal Paper | 3 February 2012 Open Access

Three-color Förster resonance energy transfer within single FOF1-ATP synthases: monitoring elastic deformations of the rotary double motor in real time

Stefan Ernst, Monika Dueser, Nawid Zarrabi, Michael Börsch

JBO, Vol. 17, Issue 1, 011004, (February 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.1.011004

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Photons, Luminescence, Resonance energy transfer, Pulsed laser operation, Confocal microscopy, Proteins, Beam splitters, Fluorescence spectroscopy

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Proceedings Article | 1 March 2011 Paper

Subunit rotation in a single FoF1-ATP synthase in a living bacterium monitored by FRET

K. Seyfert, T. Oosaka, H. Yaginuma, S. Ernst, H. Noji, R. Iino, M. Börsch

Proceedings Volume 7905, 79050K (2011) https://doi.org/10.1117/12.873066

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Luminescence, In vivo imaging, Proteins, In vitro testing, Bacteria, Molecules, Microscopes, Confocal microscopy

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Proceedings Article | 23 February 2011 Paper

Monitoring single membrane protein dynamics in a liposome manipulated in solution by the ABELtrap

T. Rendler, M. Renz, E. Hammann, S. Ernst, N. Zarrabi, M. Börsch

Proceedings Volume 7902, 79020M (2011) https://doi.org/10.1117/12.873069

KEYWORDS: Particles, Confocal microscopy, Optical tweezers, Fluorescence resonance energy transfer, Proteins, Diffusion, Electrodes, Signal to noise ratio, Cameras, Luminescence

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Proceedings Article | 23 February 2011 Paper

Drug transport mechanism of P-glycoprotein monitored by single molecule fluorescence resonance energy transfer

S. Ernst, B. Verhalen, N. Zarrabi, S. Wilkens, M. Börsch

Proceedings Volume 7903, 790328 (2011) https://doi.org/10.1117/12.872989

KEYWORDS: Fluorescence resonance energy transfer, Molecules, Acquisition tracking and pointing, Photons, Proteins, Luminescence, Pulsed laser operation, Cancer, Confocal microscopy, Quantum efficiency

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Proceedings Article | 3 March 2010 Paper

Targeting cytochrome C oxidase in mitochondria with Pt(II)-porphyrins for photodynamic therapy

Michael Börsch

Proceedings Volume 7551, 75510G (2010) https://doi.org/10.1117/12.841284

KEYWORDS: Phosphorescence, Photodynamic therapy, Proteins, Acquisition tracking and pointing, Electron transport, Oxygen, Fluorescence resonance energy transfer, In vitro testing, Microscopes, Electron microscopy

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Proceedings Article | 26 February 2010 Paper

Regulatory assembly of the vacuolar proton pump VoV1-ATPase in yeast cells by FLIM-FRET

Stefan Ernst, Claire Batisse, Nawid Zarrabi, Bettina Böttcher, Michael Börsch

Proceedings Volume 7569, 75690W (2010) https://doi.org/10.1117/12.841169

KEYWORDS: Fluorescence resonance energy transfer, Yeast, Luminescence, Glucose, Confocal microscopy, Fluorescence lifetime imaging, Acquisition tracking and pointing, Fluorescent proteins, Pulsed laser operation, Microscopes

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Proceedings Article | 25 February 2009 Paper

Simultaneous monitoring of the two coupled motors of a single FoF1-ATP synthase by three-color FRET using duty cycle-optimized triple-ALEX

N. Zarrabi, S. Ernst, M. Düser, A. Golovina-Leiker, W. Becker, R. Erdmann, S. Dunn, M. Börsch

Proceedings Volume 7185, 718505 (2009) https://doi.org/10.1117/12.809610

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Luminescence, Pulsed laser operation, Picosecond phenomena, Proteins, Confocal microscopy, Avalanche photodetectors, Bragg cells, Optical filters

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Proceedings Article | 13 February 2009 Paper

Fluorescent nanodiamonds for FRET-based monitoring of a single biological nanomotor FoF1-ATP synthase

M. Börsch, R. Reuter, G. Balasubramanian, R. Erdmann, F. Jelezko, J. Wrachtrup

Proceedings Volume 7183, 71832N (2009) https://doi.org/10.1117/12.812720

KEYWORDS: Fluorescence resonance energy transfer, Diamond, Acquisition tracking and pointing, Luminescence, Nanoparticles, Nanocrystals, Proteins, Nitrogen, Chemical species, Color centers

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Proceedings Article | 21 February 2008 Paper

Monitoring the conformational dynamics of a single potassium transporter by ALEX-FRET

N. Zarrabi, T. Heitkamp, J.-C. Greie, M. Börsch

Proceedings Volume 6862, 68620M (2008) https://doi.org/10.1117/12.768262

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Photons, Proteins, Acquisition tracking and pointing, Potassium, Monte Carlo methods, Pulsed laser operation, Ions, Particles

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Proceedings Article | 15 October 2007 Paper

Monitoring the rotary motors of single FoF1-ATP synthase by synchronized multi channel TCSPC

N. Zarrabi, M. Düser, S. Ernst, R. Reuter, G. Glick, S. Dunn, J. Wrachtrup, M. Börsch

Proceedings Volume 6771, 67710F (2007) https://doi.org/10.1117/12.734301

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Acquisition tracking and pointing, Fluorine, Pulsed laser operation, Confocal microscopy, Bragg cells, Glasses, Single photon, Microwave radiation

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Proceedings Article | 10 February 2007 Paper

Detecting substeps in the rotary motors of FoF1-ATP synthase by Hidden Markov Models

N. Zarrabi, M. Düser, R. Reuter, S. Dunn, J. Wrachtrup, M. Börsch

Proceedings Volume 6444, 64440E (2007) https://doi.org/10.1117/12.701001

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Monte Carlo methods, Computer simulations, Confocal microscopy, Particles, Luminescence, Data modeling, Diffusion, Data analysis

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Proceedings Article | 27 February 2006 Paper

3D-localization of the a-subunit in F₀F₁-ATP synthase by time resolved single-molecule FRET

Monika Düser, Nawid Zarrabi, Yumin Bi, Boris Zimmermann, Stanley Dunn, Michael Börsch

Proceedings Volume 6092, 60920H (2006) https://doi.org/10.1117/12.647988

KEYWORDS: Fluorescence resonance energy transfer, Acquisition tracking and pointing, Luminescence, Quantum efficiency, Proteins, Molecules, Confocal microscopy, Photon counting, Microscopes, Single photon

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Proceedings Article | 29 March 2005 Paper

Asymmetry of rotational catalysis of single membrane-bound F0F1-ATP synthase

Nawid Zarrabi, Boris Zimmermann, Manuel Diez, Peter Graeber, Joerg Wrachtrup, Michael Boersch

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.597967

KEYWORDS: Fluorescence resonance energy transfer, Photons, Acquisition tracking and pointing, Luminescence, Confocal microscopy, Catalysis, Fluorescence correlation spectroscopy, Single photon, Picosecond phenomena, Quantum efficiency

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Proceedings Article | 19 June 2003 Paper

Stepwise rotation of the γ-subunit of EF₀F₁-ATP synthase during ATP synthesis: a single-molecule FRET approach

Michael Borsch, Manuel Diez, Boris Zimmermann, Matthias Trost, Stefan Steigmiller, Peter Graber

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.479554

KEYWORDS: Acquisition tracking and pointing, Fluorescence resonance energy transfer, Rhodamine B, Luminescence, Catalysis, Fluorine, Confocal microscopy, Molecules, Absorbance, Diffusion

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Showing 5 of 37 publications

Conference Committee Involvement (15)

Single Molecule Spectroscopy and Superresolution Imaging XVIII

25 January 2025 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVII

27 January 2024 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XVI

28 January 2023 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XV

22 January 2022 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIV

6 March 2021 | Online Only, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XIII

1 February 2020 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XII

2 February 2019 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging XI

27 January 2018 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging X

28 January 2017 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging IX

13 February 2016 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VIII

7 February 2015 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VII

1 February 2014 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging VI

2 February 2013 | San Francisco, California, United States

Single Molecule Spectroscopy and Superresolution Imaging V

21 January 2012 | San Francisco, California, United States

Single Molecule Spectroscopy and Imaging III

23 January 2010 | San Francisco, California, United States

Showing 5 of 15 Conference Committees

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