Within 24 hours post treatment, photodynamic therapy causes a drastic reduction in tumor oxygenation and an increase in ultrasound scattering. While these trends can lead to determining acute tumor response, monitoring vascular and structural dynamics through 72 hours post treatment using ultrasound guided photoacoustic imaging provides a better insight on long-term PDT outcomes.
Obstetric risks, associated with pre-eclampsia, such as maternal age and pre-existing conditions are on the rise, along with the risk of ectopic calcification and placental vascular dysfunction. We investigated Slc20a2 mice models through non-invasive commercialized and synergistic ultrasound and photoacoustic imaging, which provide dual-wavelength images and radio frequency data. The increased ectopic placental vascular calcification model exhibits reduced fetal growth and decreased postnatal bone mineral density. Our experiments established a significant difference in both placental function and structural differences between normal and diseased placentas through the provided oxygen saturation and quantitative ultrasound spectral parameters.
KEYWORDS: Photoacoustic spectroscopy, MATLAB, Process modeling, Tissues, Monte Carlo methods, Imaging systems, Image segmentation, Visualization, Visual process modeling, Ultrasonography
This work develops a thorough and user-friendly methodology for fluence compensation of photoacoustic (PA) data from the commercial Visualsonics (VS) VevoLAZR-X System. PHANTOM (PHotoacoustic ANnotation TOolkit) has been created to help fluence compensate large PA datasets. PHANTOM provides an easy-to-use graphical user interface to assist users in segmenting US-coupled PA images into three-dimensional labeled tissue structures with assignable optical characteristics. We also modeled an MCX-compatible light source configuration that replicates the VS system's optical fiber illumination. PHANTOM exports all Monte Carlo configuration parameters which are compatible with Monte Carlo eXtreme (MCX) allowing fluence compensation of PA data.
In this work, we evaluated the acoustic and optical properties of silk protein-based hydrogels to investigate its potential as a phantom material. Acoustic properties include the speed of sound and acoustic attenuation of silk scaffolds at various concentrations. Optical properties include optical absorption and reduced scattering measured between 400 nm to 1200 nm to coincide with common photoacoustic imaging bandwidths. The results indicate that silk is an acceptable phantom material for ultrasound and photoacoustic systems as it inherently displays similar acoustic properties and reduced scattering as various tissue types while displaying low absorption.
As medical advances allow the maternal age to rise, the risk of ectopic calcification and placental vascular dysfunction increases, both associated with preeclampsia. We investigated Slc20a2 mice models through non-invasive commercial ultrasound and photoacoustic imaging. This model exhibits increased ectopic placental vascular calcification, reduced fetal growth, and decreased postnatal bone mineral density. Our experiments established a significant difference in both placental function and structural differences between normal and diseased placentas. Dual-wavelength images and radio frequency data provided oxygen saturation and quantitative ultrasound spectral parameters that proved the control placentas can be distinguished from knockout placentas.
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