Real-time intraoperative fluorescence lifetime imaging of 5-ALA induced PpIX and autofluorescence in brain tumors: first results in patients

Xiangnan Zhou; Silvia N. Anbunesan; Alba Alfonso García; Julien Bec; Lisanne Kraft; Roberto P. Frusciante; Orin Bloch; Laura Marcu

doi:10.1117/12.2612597

7 March 2022 Real-time intraoperative fluorescence lifetime imaging of 5-ALA induced PpIX and autofluorescence in brain tumors: first results in patients

Xiangnan Zhou, Silvia N. Anbunesan, Alba Alfonso García, Julien Bec, Lisanne Kraft, Roberto P. Frusciante, Orin Bloch, Laura Marcu

Author Affiliations +

Proceedings Volume PC11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII; PC1194305 (2022) https://doi.org/10.1117/12.2612597
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

5-ALA induced PpIX is increasingly used for guiding brain tumor resection surgery. The current intensity-based approach fails at detecting lower concentrations of PpIX found in low-grade gliomas or the infiltrative edge of glioblastomas. Here, we report the first results in patients of real-time, PpIX fluorescence lifetime measurements using a hand-held fiber probe. Fluorescence from different spectral channels (390/40 nm (Collagen), 470/28 nm (NADH), 629/53 nm (PPIX)), is augmented onto a video stream of the surgical field-of-view to provide intraoperative tumor visualization in real-time. In-vivo data reveals strong contrast between regions of high PpIX accumulation associated with tumor (>8 ns) and healthy brain tissue (<4 ns).

Conference Presentation

Citation Download Citation

Xiangnan Zhou, Silvia N. Anbunesan, Alba Alfonso García, Julien Bec, Lisanne Kraft, Roberto P. Frusciante, Orin Bloch, and Laura Marcu "Real-time intraoperative fluorescence lifetime imaging of 5-ALA induced PpIX and autofluorescence in brain tumors: first results in patients", Proc. SPIE PC11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, PC1194305 (7 March 2022); https://doi.org/10.1117/12.2612597

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available