Observation of tendon repair in animal model using second-harmonic-generation microscopy

Eiji Hase; Takeo Minamikawa; Katsuya Sato; Mitsuhiko Takahashi; Takashi Yasui

doi:10.1117/12.2213890

14 March 2016 Observation of tendon repair in animal model using second-harmonic-generation microscopy

Eiji Hase, Takeo Minamikawa, Katsuya Sato, Mitsuhiko Takahashi, Takashi Yasui

Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 97121R (2016) https://doi.org/10.1117/12.2213890
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Tendon rupture is a trauma difficult to recover the condition before injury. In previous researches, tensile test and staining method have been widely used to elucidate the mechanism of the repair process from the viewpoints of the mechanical property and the histological findings. However, since both methods are destructive and invasive, it is difficult to obtain both of them for the same sample. If both the mechanical property and the histological findings can be obtained from the same sample, one may obtain new findings regarding mechanisms of tendon repairing process.

In this paper, we used second-harmonic-generation (SHG) microscopy, showing high selectivity and good image contrast to collagen molecules as well as high spatial resolution, optical three-dimensional sectioning, deep penetration, and without additional staining. Since SHG light intensity sensitively reflects the structural maturity of collagen molecule and its aggregates, it will be a good indicator for the repairing degree of the ruptured tendon. From comparison of SHG images between the 4-weeks-repaired tendon and the sound tendon in the animal model, we confirmed that SHG light intensity of the repaired tendon was significantly lower than that of the sound tendon, indicating that the collagen structure in the repaired tendon is still immature. Furthermore, we performed both SHG imaging and the tensile test for the same sample, and confirmed a correlation between them. This result shows a potential of SHG light for an indicator of the histological and mechanical recovery of the ruptured tendon.

Citation Download Citation

Eiji Hase, Takeo Minamikawa, Katsuya Sato, Mitsuhiko Takahashi, and Takashi Yasui "Observation of tendon repair in animal model using second-harmonic-generation microscopy", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97121R (14 March 2016); https://doi.org/10.1117/12.2213890

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KEYWORDS

Second-harmonic generation

Collagen

Microscopy

Animal model studies

Optical fibers

Tissues

Visualization

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