Dr. Eiji Hase Profile

Dr. Eiji Hase

Assistant Professor at Tokushima Univ.

SPIE Involvement:

Author

Publications (31)

Proceedings Article | 12 March 2024 Poster + Paper

Molecular imaging analysis of lipid droplets in nonalcoholic fatty liver disease by use of Brillouin scattering microscopy

Eiji Hase, Naoya Okubo, Kazuki Yasumaru, Yu Tokizane, Takeo Minamikawa, Takeshi Yasui

Proceedings Volume 12844, 128440C (2024) https://doi.org/10.1117/12.3001190

KEYWORDS: Liver, Tissues, Microscopy, Diseases and disorders, Raman scattering, Molecules, Light scattering, Visualization, Spectroscopy, Molecular imaging

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Proceedings Article | 20 September 2023 Paper

Laser-scanning optical-frequency-comb microscopy for imaging using various aspects of light information

Shimpei Kajiwara, Takeo Minamikawa, Tomoya Okabe, Eiji Hase, Takeshi Yasui

Proceedings Volume 12608, 126081C (2023) https://doi.org/10.1117/12.3007937

KEYWORDS: Laser optics, Optical microscopy, Spectroscopy, Polarization, Imaging spectroscopy, Biological imaging, Laser applications, Polarization imaging, Interferograms, Microscopy

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Proceedings Article | 7 March 2022 Presentation

Orientation analysis of collagen fibers in skin by using polarization-resolved second-harmonic generation microscopy

Eiji Hase, Takeshi Yasui

Proceedings Volume PC11934, PC1193408 (2022) https://doi.org/10.1117/12.2613415

KEYWORDS: Collagen, Skin, Optical fibers, Microscopy, Second-harmonic generation, Visualization, Polarization, Tissues, Tissue optics, Switching

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Proceedings Article | 5 March 2021 Presentation + Paper

Dual-comb spectroscopic polarimetry for dynamic characterization of polarization property

Hidenori Koresawa, Marc Gouryeb, Kyuki Shibuya, Takahiko Mizuno, Eiji Hase, Yu Tokizane, Ryo Oe, Takeo Minamikawa, Takeshi Yasui

Proceedings Volume 11671, 116710H (2021) https://doi.org/10.1117/12.2583107

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Proceedings Article | 30 March 2020 Presentation

Laser scanning optical-frequency-comb microscopy for multivariate measurement related to amplitude and phase (Conference Presentation)

Takeo Minamikawa, Shota Nakano, Eiji Hase, Akifumi Asahara, Hidenori Koresawa, Takahiko Mizuno, Hirotsugu Yamamoto, Kaoru Minoshima, Takeshi Yasui

Proceedings Volume 11351, 113510B (2020) https://doi.org/10.1117/12.2554983

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Laser-scanning optical microscopy is widely used for the observation of microstructures and the analysis of molecular functions of samples with tightly focused light. Spectroscopic information is also available if a broadband light source is employed. General laser-scanning optical microscopy observes optical intensity by employing a sample- or laser-scanning system for the analysis of samples via reflectance, scattering, absorbance, and laser-induced phenomena. Another visualization method is using optical phase, which can enhance the image contrast of such high transparent materials and nano-step structures. However, broadband spectroscopic phase-contrast imaging with a laser-scanning configuration is slightly tricky due to the interferometric configuration is required to retrieve phase information of each wavelength. If the simultaneous measurement of amplitude and phase spectra is enabled in laser-scanning microscopy, it is possible to realize multivariate measurement to analyze more detailed information of samples based on such as complex refractive index, polarization characteristics, and so on with tightly focused light. To overcome these limitations, in this study, we proposed an optical-frequency-comb (OFC)-based laser scanning optical microscopy. The OFC technique enables fast Fourier transform spectroscopy by using well-defined two OFC lasers without any mechanical scan in the time domain. The combination of the laser scanning optical microscopy and the OFC technique realized the simultaneous and spectroscopic observation of quantitative amplitude and phase images with tight focusing down to the diffraction limit. Furthermore, we realized the analysis of polarization by the direct observation of the amplitude and phase of the orthogonal components. We applied the proposed method to the observation of nano-step structures, phase objects and anisotropic materials to provide a proof-of-principle demonstration of the proposed method. Our proposed approach will serve as a unique and powerful tool for characterizing the materials via complete characterization of optical information such as amplitude, phase, polarization and spectrum.

Proceedings Article | 4 March 2019 Paper

Scan-less confocal phase imaging of biological samples using dual-comb microscopy

Takuya Tsuda, Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto, Takeshi Yasui

Proceedings Volume 10889, 108891B (2019) https://doi.org/10.1117/12.2509244

KEYWORDS: Confocal microscopy, Phase imaging, Frequency combs

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SPIE Journal Paper | 11 January 2019 Open Access

Quantitative in situ time-series evaluation of osteoblastic collagen synthesis under cyclic strain using second-harmonic-generation microscopy

Katsuya Sato, Oki Matsubara, Eiji Hase, Takeo Minamikawa, Takeshi Yasui

JBO, Vol. 24, Issue 03, 031019, (January 2019) https://doi.org/10.1117/12.10.1117/1.JBO.24.3.031019

KEYWORDS: Collagen, Second-harmonic generation, Optical fibers, Microscopy, Bone, Regenerative medicine, CMYK color model, Signal detection, Pulsed laser operation, Image analysis

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