Dr. Benedikt Krämer Profile

Dr. Benedikt Krämer

Senior Scientist Microscopy at PicoQuant GmbH

SPIE Involvement:

Author

Publications (20)

Proceedings Article | 17 March 2023 Poster

One pattern analysis for the quantitative determination of protein interactions in living plant cells

Felix Koberling, Jan Maika, Benedikt Krämer, Frank Wellmer, Stefanie Weidtkamp-Peters, Yvonne Stahl, Rüdiger Simon Simon

Proceedings Volume PC12384, PC1238412 (2023) https://doi.org/10.1117/12.2651434

KEYWORDS: Proteins, Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Visualization, Molecules, In vivo imaging, Cell mechanics

Read Abstract +

Proceedings Article | 26 March 2020 Presentation

Scanning FCS and super-resolution microscopy on 2D lipid membranes (Conference Presentation)

Uwe Ortmann, Mariano Gonzalez Pisfil, Marcelle König, Rhys Dowler, Benedikt Krämer, Sumeet Rohilla, Felix Koberling, Rainer Erdmann

Proceedings Volume 11246, 1124607 (2020) https://doi.org/10.1117/12.2542417

KEYWORDS: Fluorescence correlation spectroscopy, Super resolution microscopy, Stimulated emission depletion microscopy, Diffusion, Cell mechanics, Molecules, Calibration, Confocal microscopy, Time resolved spectroscopy, Microscopes

Read Abstract +

Proceedings Article | 9 March 2020 Presentation

Multi-target immunofluorescence using spectral FLIM-FRET for separation of undesirable antibody cross-labeling and autofluorescence (Conference Presentation)

Sumeet Rohilla, Benedikt Kraemer, Felix Koberling, Uwe Ortmann, Ingo Gregor, Andreas Hocke, Rainer Erdmann

Proceedings Volume 11244, 1124418 (2020) https://doi.org/10.1117/12.2542413

KEYWORDS: Molecules, Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Molecular interactions, Luminescence, Electronics, Data analysis, Time resolved spectroscopy, Lung, Tissues

Read Abstract +

Proceedings Article | 22 July 2019 Paper

Multi-target immunofluorescence using spectral FLIM-FRET for separation of undesirable antibody cross-labeling

Sumeet Rohilla, Benedikt Krämer, Felix Koberling, Ingo Gregor, Andreas Hocke

Proceedings Volume 11076, 1107618 (2019) https://doi.org/10.1117/12.2526240

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Data acquisition, Control systems, Fluorescence lifetime imaging, Pulsed laser operation, Molecules, Statistical analysis, Biological research, Chemical analysis

Read Abstract +

Proceedings Article | 11 July 2019 Paper

Large area SiPM and high throughput timing electronics: toward new generation time-domain instruments

Anurag Behera, Laura Di Sieno, Sumeet Rohilla, Antonio Pifferi, Alessandro Torricelli, Davide Contini, Benedikt Krämer, Felix Koberling, Alberto Dalla Mora

Proceedings Volume 11074, 1107402 (2019) https://doi.org/10.1117/12.2526792

KEYWORDS: Electronics, Sensors, Absorption, Optical properties, Scattering, Tissue optics, Picosecond phenomena, In vivo imaging, Brain activation, Signal detection

Read Abstract +

Proceedings Article | 4 March 2019 Presentation

Spectrally resolved and high speed TCSPC-based fluorescence lifetime imaging (Conference Presentation)

Tino Roehlicke, Matthias Patting, Hans-Juergen Rahn, Benedikt Kraemer, Michael Wahl, Felix Koberling, Rainer Erdmann

Proceedings Volume 10889, 108890Q (2019) https://doi.org/10.1117/12.2513895

KEYWORDS: Fluorescence lifetime imaging, Signal detection, Sensors, Live cell imaging, Single photon, Luminescence, Electronics, Image acquisition, Picosecond phenomena, Confocal microscopy

Read Abstract +

Proceedings Article | 4 March 2019 Presentation

Quantitative ultra-fast FLIM and lifetime based multi-species analysis (Conference Presentation)

Benedikt Kraemer, Sumeet Rohilla, Rhys Dowler, Marcus Sackrow, Matthias Patting, Hans-Jürgen Rahn, Michael Wahl, Uwe Ortmann, Felix Koberling, Andreas Hocke, Rainer Erdmann

Proceedings Volume 10882, 108820K (2019) https://doi.org/10.1117/12.2507396

KEYWORDS: Fluorescence lifetime imaging, Ultrafast phenomena, Tissues, Image processing, Luminescence, Confocal microscopy, Life sciences, Photomultipliers, Sensors, Spatial resolution

Read Abstract +

Proceedings Article | 14 March 2018 Presentation

Scanning FCS and multi-species STED microscopy for diffusion studies in membranes (Conference Presentation)

Felix Koberling, Mariano Gonzalez Pisfil, Rhys Dowler, Marcelle Koenig, Benedikt Kraemer, Paja Reisch, Matthias Patting

Proceedings Volume 10500, 1050006 (2018) https://doi.org/10.1117/12.2289677

KEYWORDS: Fluorescence correlation spectroscopy, Stimulated emission depletion microscopy, Diffusion, Molecules, Microscopy, Molecular spectroscopy, Confocal microscopy, Molecular interactions, Microscopes, Single photon

Read Abstract +

Proceedings Article | 24 April 2017 Presentation

Rapid FLIM: The new and innovative method for ultra-fast imaging of biological processes (Conference Presentation)

Sandra Orthaus-Mueller, Benedikt Kraemer, Astrid Tannert, Tino Roehlicke, Michael Wahl, Hans-Juergen Rahn, Felix Koberling, Rainer Erdmann

Proceedings Volume 10069, 1006919 (2017) https://doi.org/10.1117/12.2249757

KEYWORDS: Ultrafast phenomena, Data acquisition, Sensors, Photons, Fluorescence lifetime imaging, Signal processing, Single photon, Fluorescence resonance energy transfer, Image processing, Time resolved spectroscopy

Read Abstract +

Proceedings Article | 14 March 2016 Paper

ns-time resolution for multispecies STED-FLIM and artifact free STED-FCS

Marcelle Koenig, Paja Reisch, Rhys Dowler, Benedikt Kraemer, Sebastian Tannert, Matthias Patting, Mathias Clausen, Silvia Galiani, Christian Eggeling, Felix Koberling, Rainer Erdmann

Proceedings Volume 9712, 97120T (2016) https://doi.org/10.1117/12.2208874

KEYWORDS: Fluorescence correlation spectroscopy, Super resolution, Microscopy, Pulsed laser operation, Luminescence, Picosecond phenomena, Stimulated emission depletion microscopy, Optical resolution, Time correlated photon counting, Single photon, Diffusion, Confocal microscopy, Molecules, Diffraction

Read Abstract +

Proceedings Article | 11 February 2011 Paper

Fast algorithms for the analysis of spectral FLIM data

I. Gregor, B. Krämer, F. Koberling, R. Erdmann, J. Enderlein, M. Wahl, S. Fore

Proceedings Volume 7903, 790330 (2011) https://doi.org/10.1117/12.873386

KEYWORDS: Fluorescence lifetime imaging, Luminescence, Fluorescence resonance energy transfer, Signal detection, Data conversion, Single photon, Picosecond phenomena, Microscopes, Statistical analysis, Fluorescence spectroscopy

Read Abstract +

Proceedings Article | 25 February 2009 Paper

Recent advances in photon coincidence measurements for photon antibunching and full correlation analysis

F. Koberling, B. Kraemer, V. Buschmann, S. Ruettinger, P. Kapusta, M. Patting, M. Wahl, R. Erdmann

Proceedings Volume 7185, 71850Q (2009) https://doi.org/10.1117/12.807803

KEYWORDS: Molecules, Signal detection, Sensors, Picosecond phenomena, Luminescence, Fluorescence correlation spectroscopy, Diffusion, Confocal microscopy, Electronics, Time metrology

Read Abstract +

Proceedings Article | 21 February 2008 Paper

Recent advances in time-correlated single-photon counting

Felix Koberling, Benedikt Krämer, Sebastian Tannert, Steffen Rüttinger, Uwe Ortmann, Matthias Patting, Michael Wahl, Benjamin Ewers, Peter Kapusta, Rainer Erdmann

Proceedings Volume 6862, 686209 (2008) https://doi.org/10.1117/12.761161

KEYWORDS: Fluorescence correlation spectroscopy, Photons, Luminescence, Sensors, Diffusion, Pulsed laser operation, Picosecond phenomena, Confocal microscopy, Molecules, Light scattering

Read Abstract +

Proceedings Article | 15 February 2008 Paper

Advanced FRET and FCS measurements with laser scanning microscopes based on time-resolved techniques

B. Krämer, V. Buschmann, U. Ortmann, F. Koberling, M. Wahl, M. Patting, P. Kapusta, A. Bülter, R. Erdmann

Proceedings Volume 6860, 68601D (2008) https://doi.org/10.1117/12.761141

KEYWORDS: Sensors, Luminescence, Fluorescence correlation spectroscopy, Fluorescence resonance energy transfer, Pulsed laser operation, Laser scanners, Microscopes, Single photon, Data acquisition, Fluorescence lifetime imaging

Read Abstract +

Proceedings Article | 12 July 2007 Paper

Determination of the confocal volume for quantitative fluorescence correlation spectroscopy

S. Rüttinger, V. Buschmann, B. Krämer, R. Erdmann, R. Macdonald, F. Koberling

Proceedings Volume 6630, 66300D (2007) https://doi.org/10.1117/12.728463

KEYWORDS: Fluorescence correlation spectroscopy, Confocal microscopy, Diffusion, Particles, Molecules, Luminescence, Microscopes, Data modeling, Point spread functions, Adsorption

Read Abstract +

Proceedings Article | 11 May 2007 Paper

Time-resolved confocal fluorescence microscopy: novel technical features and applications for FLIM, FRET and FCS using a sophisticated data acquisition concept in TCSPC

Felix Koberling, Benedikt Krämer, Peter Kapusta, Matthias Patting, Michael Wahl, Rainer Erdmann

Proceedings Volume 6583, 65830Y (2007) https://doi.org/10.1117/12.722867

KEYWORDS: Fluorescence resonance energy transfer, Luminescence, Molecules, Fluorescence correlation spectroscopy, Confocal microscopy, Fluorescence lifetime imaging, Data acquisition, Molecular photonics, Microscopy, Picosecond phenomena

Read Abstract +

In recent years time-resolved fluorescence measurement and analysis techniques became a standard in single molecule microscopy. However, considering the equipment and experimental implementation they are typically still an add-on and offer only limited possibilities to study the mutual dependencies with common intensity and spectral information. In contrast, we are using a specially designed instrument with an unrestricted photon data acquisition approach which allows to store spatial, temporal, spectral and intensity information in a generalized format preserving the full experimental information. This format allows us not only to easily study dependencies between various fluorescence parameters but also to use, for example, the photon arrival time for sorting and weighting the detected photons to improve the significance in common FCS and FRET analysis schemes. The power of this approach will be demonstrated for different techniques: In FCS experiments the concentration determination accuracy can be easily improved by a simple time-gated photon analysis to suppress the fast decaying background signal. A more detailed analysis of the arrival times allows even to separate FCS curves for species which differ in their fluorescence lifetime but, for example, cannot be distinguished spectrally. In multichromophoric systems like a photonic wire which undergoes unidirectional multistep FRET the lifetime information complements significantly the intensity based analysis and helps to assign the respective FRET partners. Moreover, together with pulsed excitation the time-correlated analysis enables directly to take advantage of alternating multi-colour laser excitation. This pulsed interleaved excitation (PIE) can be used to identify and rule out inactive FRET molecules which cause interfering artefacts in standard FRET efficiency analysis. We used a piezo scanner based confocal microscope with compact picosecond diode lasers as excitation sources. The timing performance can be significantly increased by using new SPAD detectors which enable, in conjunction with new TCSPC electronics, an overall IRF width of less than 120 ps maintaining single molecule sensitivity.

SPIE Journal Paper | 1 March 2006 Open Access

Accurate single-pair Förster resonant energy transfer through combination of pulsed interleaved excitation, time correlated single-photon counting, and fluorescence correlation spectroscopy

Steffen Rüttinger, Rainer Macdonald, Benedikt Krämer, Felix Koberling, Martin Roos, Eberhardt Hildt

JBO, Vol. 11, Issue 02, 024012, (March 2006) https://doi.org/10.1117/12.10.1117/1.2187425

KEYWORDS: Fluorescence resonance energy transfer, Molecules, Luminescence, Photons, Fluorescence correlation spectroscopy, Confocal microscopy, Resonance energy transfer, Energy efficiency, Molecular energy transfer, Quantum efficiency

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 27 February 2006 Paper

Measuring precise diffusion coefficients with two-focus fluorescence correlation spectroscopy

Thomas Dertinger, Ingo Gregor, Iris von der Hocht, Rainer Erdmann, Benedikt Krämer, Felix Koberling, Rudolf Hartmann, Jörg Enderlein

Proceedings Volume 6092, 609203 (2006) https://doi.org/10.1117/12.651053

KEYWORDS: Diffusion, Fluorescence correlation spectroscopy, Luminescence, Molecules, Pulsed laser operation, Polarization, Beam splitters, Objectives, Photodetectors, Data modeling

Read Abstract +

Proceedings Article | 30 March 2005 Paper

Time-resolved laser scanning microscopy with FLIM and advanced FCS capability

Benedict Kraemer, Felix Koberling, Uwe Ortmann, Michael Wahl, Peter Kapusta, Andreas Buelter, Rainer Erdmann

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.590500

KEYWORDS: Laser scanners, Data acquisition, Microscopes, Picosecond phenomena, Sensors, Luminescence, Fluorescence lifetime imaging, Fluorescence correlation spectroscopy, Semiconductor lasers, Microscopy

Read Abstract +

We present the technical integration of state-of-the-art picosecond diode laser sources and data acquisition electronics in conventional laser scanning microscopes. This procedure offers users of laser scanning microscopes an easy upgrade path towards time-resolved measurements. Our setup uses picosecond diode lasers from 375 to 800 nm for excitation which are coupled to the microscope via a single mode fiber. The corresponding emission is guided to a fibre coupled photon counting detector, such as Photomultiplier Tubes (PMT) or Single Photon Avalanche Diodes (SPAD). This combines the outstanding sensitivity of photon counting detectors with the ease of use of diode laser sources, to allow time-resolved measurements of fluorescence decays with resolutions down to picoseconds. The synchronization signals from the laser scanning microscope are fed into the data stream recorded by the TimeHarp 200 TCSPC system, via the unique Time-Tagged Time-Resolved (TTTR) data acquisition mode. In this TTTR data acquisition mode each photon is recorded individually with its specific parameters as detector channel, picosecond timing, global arrival time and, in this special application, up to three additional markers. These markers, in combination with the global arrival time, allow the system software to reconstruct the complete image and subsequently create the full fluorescence lifetime image (FLIM). The multi-parameter data acquisition scheme of the TimeHarp 200 electronics not only records each parameter individually, but offers in addition the opportunity to analyse the parameter dependencies in a multitude of different ways. This method allows for example to calculate the fluorescence fluctuation correlation function (FCS) on any single spot of interest but also to reconstruct the fluorescence decay of each image pixel and detector channel for advanced Forster Resonance Energy Transfer (FRET) analysis. In this paper, we present some selected results acquired with standard laser scanning microscopes upgraded towards temporal resolution.

Proceedings Article | 25 August 1997 Paper

Flexible data registration and automation in semiconductor production

Ralf Dudde, Peter Staudt-Fischbach, Benedict Kraemer

Proceedings Volume 3213, (1997) https://doi.org/10.1117/12.284635

KEYWORDS: Semiconductors, Semiconducting wafers, Control systems, Data modeling, Databases, Telecommunications, Computing systems, Interfaces, Human-machine interfaces, Silicon

Read Abstract +

Showing 5 of 20 publications

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years