Dr. Kristian Berg Profile

Dr. Kristian Berg

Senior Scientist at Oslo Univ Hospital

SPIE Involvement:

Author

Publications (19)

Proceedings Article | 24 June 2021 Presentation

Detection of chemiluminescence-induced photosensitizer activation through fluorescence and concomitant singlet oxygen generation

Theodossis Theodossiou, Mantas Grigalavicius, Kristian Berg

Proceedings Volume 11786, 117860B (2021) https://doi.org/10.1117/12.2600610

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In the course of experiments for our FET open project Lumiblast, we set off to measure the excitation of various photoactive drugs (photosensitizers, PS) by the luminescence emission of luminol. Luminol (5-Amino-2,3-dihydrophthalazine-1,4-dione) is a chemical that interacts with reactive oxygen species (ROS) in basic conditions, and in the presence of metal catalysts like Fe or Cu, gives out a characteristic blue luminescence. When dissolved in organic solvents like DMSO, however, luminol only requires the addition of bases like KOH, NaOH or potassium terbutoxide, to fulfil the conditions for luminescence emission. In the present work we employed a detection system based on a spectrograph coupled to a ccd camera to register fluorescence (Fig 1B) or luminescence (Fig 1 A, C). In the case of characteristic fluorescence registration (Fig. 1B), the PSs investigated were excited by a 532 nm laser with a variable power output. We have documented the energy transfer from chemically induced luminol luminescence to a number of PSs including rose bengal, erythrosin B, hypericin amongst others. In all cases both the luminol emission and the luminol luminescence-induced PS fluorescence were registered as shown in the example of luminol and erythrosine b in Fig. 1C. We further attempted to register the generation of singlet oxygen from luminol-excited PSs. To achieve this, we employed the near-infrared (NIR) photomultiplier tube (PMT) shown in Fig.1 E, with a cut-off filter at 900nm and a bandpass filter at 1270±30 nm. This allowed only radiation within this spectral region to reach the PMT, corresponding to the characteristic phosphorescence of singlet oxygen, spin forbidden de-excitation to ground state triplet oxygen. A characteristic steady state singlet oxygen registration can be seen in Fig. 1D, for erythrosine b which has a high singlet oxygen quantum yield. The luminol luminescence was initiated by addition of terbutoxide to the DMSO luminol solution, at which point we can see a rise of the signal at 1270 nm. Upon addition of the singlet oxygen quencher, L-histidine, the signal dropped steeply to background levels. NOTE: Figures are not available.

Proceedings Article | 14 August 2019 Presentation

Photochemical internalization (PCI) of bleomycin induces T-cell activation of importance for curative effect and systemic anti-tumor immunity (Conference Presentation)

Kristian Berg, Ane Sofie Fremstedal, Ole-Jacob Norum, Anette Weyergang, Jakub Golab

Proceedings Volume 11070, 110702P (2019) https://doi.org/10.1117/12.2526088

KEYWORDS: Tumors, Colon, Semiconductor lasers, Spleen

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Proceedings Article | 14 August 2019 Presentation

The scientific basis of photochemical internalization (PCI) (Conference Presentation)

Kristian Berg

Proceedings Volume 11070, 110703A (2019) https://doi.org/10.1117/12.2527907

KEYWORDS: Picosecond phenomena, Diffusion, Oxygen, Luminescence, Photodynamic therapy, Cancer, Oncology

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Proceedings Article | 10 March 2015 Paper

Macrophage mediated PCI enhanced gene-directed enzyme prodrug therapy

Henry Hirschberg, Catherine Christie, Genesis Zamora, Kristian Berg, Steen Madsen, Young Kwon

Proceedings Volume 9305, 93050C (2015) https://doi.org/10.1117/12.2082590

KEYWORDS: Tumors, Nose, Imaging systems, Linear filtering, Sensors, Digital imaging, Detection and tracking algorithms, Channel projecting optics, Convolution, Matrices

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SPIE Journal Paper | 23 October 2014 Open Access

Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier

Genesis Zamora, Frederick Wang, Chung-Ho Sun, Anthony Trinidad, Young Jik Kwon, Soo Kyung Cho, Kristian Berg, Steen Madsen, Henry Hirschberg

JBO, Vol. 19, Issue 10, 105009, (October 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.10.105009

KEYWORDS: Tumors, Control systems, Nanoparticles, Photodynamic therapy, Neptunium, Toxicity, Brain, Cancer, In vitro testing, Magnesium

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Proceedings Article | 5 March 2014 Paper

Photochemical internalization (PCI) enhanced nonviral transfection of tumor suppressor and pro-drug activating genes; a potential treatment modality for gliomas

Genesis Zamora, Anthony Trinidad, Young Jik Kwon, Henry Hirschberg, Frederick Wang, Kristian Berg, Steen Madsen, Chung-Ho Sun

Proceedings Volume 8928, 89280B (2014) https://doi.org/10.1117/12.2041627

KEYWORDS: Tumors, Photodynamic therapy, In vitro testing, Cancer, Macromolecules, Green fluorescent protein, Picosecond phenomena, Nanoparticles, OSLO, Brain

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Proceedings Article | 23 March 2007 Paper

Photochemical internalization for the treatment of malignant gliomas

Steen Madsen, Kristian Berg, Henry Hirschberg, Khishigzaya Kharkhuu

Proceedings Volume 6424, 64242C (2007) https://doi.org/10.1117/12.705086

KEYWORDS: Macromolecules, Toxicity, Cancer, Photodynamic therapy, Luminescence, Oxygen, In vitro testing, Medicine, Lanthanum, Laser therapeutics

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Proceedings Article | 3 February 1999 Paper

Characterization of the cellular uptake of 5-aminolevulinic acid

Kristian Berg, Eva Rud

Proceedings Volume 3563, (1999) https://doi.org/10.1117/12.339116

KEYWORDS: Cancer, Proteins, Photodynamic therapy, Oncology, Tumor growth modeling, Systems modeling, Mode conditioning cables, Tumors, Plasma, Colorectal cancer

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Proceedings Article | 29 December 1997 Paper

Different pathways to apoptosis induced by tetraphenylporphine derivatives and light in V79 cells

Jahn Nesland, Barbara Noodt, Trond Stokke, Kristian Berg, Qian Peng

Proceedings Volume 3191, (1997) https://doi.org/10.1117/12.297791

KEYWORDS: Cell death, Photodynamic therapy, Model-based design, Flow cytometry

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Proceedings Article | 29 December 1997 Paper

In-vitro studies of zinc(II)-phthalocyanine (Zn-PC) for use in photochemotherapy

G. Rodal, Kristian Berg, Johan Moan

Proceedings Volume 3191, (1997) https://doi.org/10.1117/12.297816

KEYWORDS: In vitro testing, Cervix

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Proceedings Article | 29 December 1997 Paper

In-situ localization of a photosensitizer as evaluated by vital microscopy

Kristian Berg, Johan Moan, Tor Brustad, Torhild Voss, Pal Selbo

Proceedings Volume 3191, (1997) https://doi.org/10.1117/12.297802

KEYWORDS: Tissue optics, Microscopy, Tumors, Tissues, Cameras, Astronomical imaging, Colon, In vitro testing, Magnesium, In vivo imaging

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Proceedings Article | 4 December 1996 Paper

Combined treatment of photodynamic therapy and the topoisomerase I inhibitor camptothecin in growing V79 and NHIK 3025 cells

Siv Rodal, Kristian Berg, Johan Moan, Jean-Michel Gaullier

Proceedings Volume 2924, (1996) https://doi.org/10.1117/12.260775

KEYWORDS: Photodynamic therapy, Cervix, Lung, Luminescence, Tissues, Microscopes, OSLO, Cancer, Tumors, Microelectromechanical systems

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Proceedings Article | 31 January 1996 Paper

Biodistribution, pharmacokinetic, and in-vivo fluorescence spectroscopic studies of photosensitizers

Johan Moan, Magne Kongshaug, Richard Horobin, Vladimir Iani, Kristian Berg, Jahn Nesland, Qian Peng, Li Wei Ma

Proceedings Volume 2625, (1996) https://doi.org/10.1117/12.231008

KEYWORDS: Tumors, Tissues, Luminescence, Proteins, Plasma, Skin, In vivo imaging, Forward error correction, Fluorescence spectroscopy, Spectroscopy

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Proceedings Article | 31 January 1996 Paper

Enhancement of the efficiency of photodynamic therapy by combination with the microtubule inhibitor vincristine

Johan Moan, Kristian Berg, Vladimir Iani, Havard Danielsen, Li Wei Ma

Proceedings Volume 2625, (1996) https://doi.org/10.1117/12.230984

KEYWORDS: Tumors, Photodynamic therapy, Tissues, Cancer, Microscopes, Integrated optics, Tumor growth modeling, Image analysis, Optical testing, Oncology

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Proceedings Article | 12 January 1995 Paper

Primary targets in photochemical inactivation of cells in culture

Stuart Jones, Kristian Prydz, Kristian Berg, Johan Moan

Proceedings Volume 2325, (1995) https://doi.org/10.1117/12.199143

KEYWORDS: Cancer, Photodynamic therapy, OSLO, Adaptive optics, Polymerization, Biophysics, Biochemistry, Oncology, Clinical trials, Proteins

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Proceedings Article | 12 January 1995 Paper

Photomodification of ALA-induced protoporphyrin IX in cells in vitro

Giedre Streckyte, Kristian Berg, Johan Moan

Proceedings Volume 2325, (1995) https://doi.org/10.1117/12.199173

KEYWORDS: Luminescence, Oxygen, Cancer, Photodynamic therapy, In vitro testing, Spectroscopy, Tissue optics, Tissues, Light, OSLO

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Proceedings Article | 12 January 1995 Paper

Combination of photodynamic therapy and x-irradiation: a study on 5-aminolevulinic acid (ALA) radiomodifying properties

Zivile Luksiene, Kristian Berg, Johan Moan

Proceedings Volume 2325, (1995) https://doi.org/10.1117/12.199159

KEYWORDS: Photodynamic therapy, X-rays, Ionizing radiation, Cancer, Radiotherapy, Gamma radiation, Fluorescence correlation spectroscopy, OSLO, Tissues, In vivo imaging

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Proceedings Article | 1 March 1994 Paper

Effect of sulfonation on the photosensitizing efficiency of aluminum phthalocyanine and meso-tetraphenylporphine in photodynamic therapy of cancer

Kristian Berg, Li Wei Ma, Johan Moan, Qian Peng

Proceedings Volume 2078, (1994) https://doi.org/10.1117/12.168662

KEYWORDS: Tumors, Photodynamic therapy, Tissues, Aluminum, Cancer, Tissue optics, Oxygen, Skin, Laser irradiation, Molecules

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Proceedings Article | 1 March 1994 Paper

Cellular responses to photodynamic therapy

Kristian Berg, Qian Peng, Johan Moan, Jahn Nesland

Proceedings Volume 2078, (1994) https://doi.org/10.1117/12.168664

KEYWORDS: Photodynamic therapy, Acquisition tracking and pointing, Plasma, Luminescence, Mode conditioning cables, Tumors, Cancer, Oxygen, Microscopy, Photomicroscopy

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