Mr. Min-Su Kim Profile

Min-Su Kim

Student at Seoul National Univ

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Publications (3)

Proceedings Article | 2 May 2006 Paper

The verification of UAV's autopilot system with realtime HILS

Min-Soo Kim, Hyeong-Jik Kong, Soo-Ho Baek, Yu-Hwan Choe, Sung-Kyung Hong

Proceedings Volume 6042, 60422I (2006) https://doi.org/10.1117/12.664661

KEYWORDS: Computer simulations, Unmanned aerial vehicles, Control systems, Fluorescence correlation spectroscopy, Control systems design, Actuators, Sensors, Computing systems, Algorithm development, Digital signal processing

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Proceedings Article | 19 January 2006 Paper

Silicon-on-glass based microchip for protein sensing and analysis by confocal microscopy and MALDI-TOF

M. Kim, S. Cho, B. Kim, Y. Kim

Proceedings Volume 6036, 60360Z (2006) https://doi.org/10.1117/12.638733

KEYWORDS: Proteins, Luminescence, Silicon, Confocal microscopy, Microfluidics, Glasses, Biological research, Fluorescence spectroscopy, Analytical research, Statistical analysis

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We propose a prototype of silicon-on-glass microchip for protein detection by bead-based affinity chromatography. The microchip has five channels integrated by composing one beads reactor per one channel. Especially, an effective protein analysis mechanism is presented where the three protein-pretreatment processes are simultaneously performed on a single beads reactor: selective detection (purification / sensing), pre-concentration and protein digestion. Since the five channels are closely spaced in parallel on the microchip, it is possible to inspect the five different detection results on real-time in a single microscope image. The microchip is fabricated on silicon-on-glass (SiOG) to make a mechanically strong and vertically transparent structure for efficient fluid interconnection and fluorescence detection, respectively. Within the microchip, the grid-type filter is formed on channel output to physically trap 38 ~ 50 μm diameter microbeads. The dimension of one grid is 30 × 30 μm². The volume flow rate was investigated experimentally on the case of bead-packed chamber, and the resulted value was compared to that of the case of hollow chamber. In this research, we used self-cleavage free aptazymes as detection ligands immobilized on polystyrene microbeads. The target proteins are firstly on-chip concentrated and fluorescence-detected (confocal microscopy), and secondly checked off-chip by using MALDI-TOF. If the two analyses are used cooperatively, it is expected that the accuracy in diagnostic analysis will be enhanced in biosensing system. Especially by using this free aptazymes system, we don't need to consider the requirement of fluorescence tagging and the difficulty of eluting antibody-bound proteins from microbeads without bad effects of harsh elution conditions in protease treatment. We analyzed the on-bead detection of HCV replicase and HCV helicase respectively by measuring fluorescence intensities at different concentrations, and also performed a selectively detection of HCV helicase from protein mixtures.

Proceedings Article | 19 January 2006 Paper

A microfabricated device with integrated nanoelectrospray source for capillary electrophoresis and mass spectrometry

Min-Su Kim, Hwang-soo Joo, Byung-Gee Kim, Hoseong Kim, Yong-Kweon Kim

Proceedings Volume 6036, 60361K (2006) https://doi.org/10.1117/12.638735

KEYWORDS: Glasses, Spectroscopy, Liquids, Gold, Microfluidics, Capillaries, Metals, Ions, Electroplating, Interfaces

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