Prof. Yong-Kweon Kim Profile

Prof. Yong-Kweon Kim

Professor at Seoul National Univ

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Author

Publications (20)

Proceedings Article | 19 January 2006 Paper

Silicon-on-glass based microchip for protein sensing and analysis by confocal microscopy and MALDI-TOF

M. Kim, S. Cho, B. Kim, Y. Kim

Proceedings Volume 6036, 60360Z (2006) https://doi.org/10.1117/12.638733

KEYWORDS: Proteins, Luminescence, Silicon, Confocal microscopy, Microfluidics, Glasses, Biological research, Fluorescence spectroscopy, Analytical research, Statistical analysis

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We propose a prototype of silicon-on-glass microchip for protein detection by bead-based affinity chromatography. The microchip has five channels integrated by composing one beads reactor per one channel. Especially, an effective protein analysis mechanism is presented where the three protein-pretreatment processes are simultaneously performed on a single beads reactor: selective detection (purification / sensing), pre-concentration and protein digestion. Since the five channels are closely spaced in parallel on the microchip, it is possible to inspect the five different detection results on real-time in a single microscope image. The microchip is fabricated on silicon-on-glass (SiOG) to make a mechanically strong and vertically transparent structure for efficient fluid interconnection and fluorescence detection, respectively. Within the microchip, the grid-type filter is formed on channel output to physically trap 38 ~ 50 μm diameter microbeads. The dimension of one grid is 30 × 30 μm². The volume flow rate was investigated experimentally on the case of bead-packed chamber, and the resulted value was compared to that of the case of hollow chamber. In this research, we used self-cleavage free aptazymes as detection ligands immobilized on polystyrene microbeads. The target proteins are firstly on-chip concentrated and fluorescence-detected (confocal microscopy), and secondly checked off-chip by using MALDI-TOF. If the two analyses are used cooperatively, it is expected that the accuracy in diagnostic analysis will be enhanced in biosensing system. Especially by using this free aptazymes system, we don't need to consider the requirement of fluorescence tagging and the difficulty of eluting antibody-bound proteins from microbeads without bad effects of harsh elution conditions in protease treatment. We analyzed the on-bead detection of HCV replicase and HCV helicase respectively by measuring fluorescence intensities at different concentrations, and also performed a selectively detection of HCV helicase from protein mixtures.

Proceedings Article | 19 January 2006 Paper

A microfabricated device with integrated nanoelectrospray source for capillary electrophoresis and mass spectrometry

Min-Su Kim, Hwang-soo Joo, Byung-Gee Kim, Hoseong Kim, Yong-Kweon Kim

Proceedings Volume 6036, 60361K (2006) https://doi.org/10.1117/12.638735

KEYWORDS: Glasses, Spectroscopy, Liquids, Gold, Microfluidics, Capillaries, Metals, Ions, Electroplating, Interfaces

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Proceedings Article | 19 January 2006 Paper

Thermal denaturation and trypsin digestion of protein on a temperature controllable microchip for MALDI-TOF MS analysis

Tae Sim, Eun-Mi Kim, Hwang Joo, Byung Kim, Hoseung Kim, Yong-Kweon Kim

Proceedings Volume 6036, 60361J (2006) https://doi.org/10.1117/12.638711

KEYWORDS: Proteins, Platinum, Sensors, Silicon, Electrodes, Resistance, Control systems, Statistical analysis, Data acquisition, Chemical analysis

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Proceedings Article | 30 December 2004 Paper

Single crystalline silicon micromirror array for maskless photolithography in protein synthesis systems

Yun-Ho Jang, Kook-Nyung Lee, Dong-Sik Shin, Yoon-Sik Lee, Yong-Kweon Kim

Proceedings Volume 5641, (2004) https://doi.org/10.1117/12.574010

KEYWORDS: Mirrors, Semiconducting wafers, Silicon, Micromirrors, Electrodes, Optical lithography, Aluminum, Proteins, Glasses, Crystals

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Proceedings Article | 30 December 2004 Paper

Silicon scanning mirror with 54.74-degree slanted reflective surface for fluorescence scanning system

Kook-Nyung Lee, Yun-Ho Jang, Jaeho Choi, Hoseoung Kim, Yoon-Sik Lee, Yong-Kweon Kim

Proceedings Volume 5641, (2004) https://doi.org/10.1117/12.575974

KEYWORDS: Mirrors, Silicon, Optical benches, Prisms, Deep reactive ion etching, Photoresist materials, Reflectivity, Electrodes, Optical fibers, Semiconducting wafers

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Proceedings Article | 28 September 2001 Paper

Photochemical selective surface modification using micromirror array for biochip fabrication

Kook-Nyung Lee, Dong-Sik Shin, Woo-Jae Chung, Yoon-Sik Lee, Yong-Kweon Kim

Proceedings Volume 4557, (2001) https://doi.org/10.1117/12.442965

KEYWORDS: Proteins, Optical lithography, Micromirrors, Ultraviolet radiation, Glasses, Bandpass filters, Luminescence, Mirrors, Photomasks, Photoresist materials

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Proceedings Article | 28 September 2001 Paper

Modeling of the comb actuator deformed by microloading effect in deep reactive ion etching

June-Young Lee, Seong-Hyok Kim, Hyung-Taek Lim, Che-Heung Kim, Yong-Kweon Kim

Proceedings Volume 4557, (2001) https://doi.org/10.1117/12.442952

KEYWORDS: Electrodes, Actuators, Deep reactive ion etching, Capacitance, Etching, Silicon, Sensors, Virtual colonoscopy, Electrical engineering, Computer science

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Proceedings Article | 28 September 2001 Paper

Fabrication of electromagnetic micromirror array

YunHo Jang, Yong-Kweon Kim

Proceedings Volume 4557, (2001) https://doi.org/10.1117/12.442971

KEYWORDS: Mirrors, Micromirrors, Magnetism, Aluminum, Electromagnetism, Nickel, Electroplating, Reflection, Photoresist materials, Micromachining

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Proceedings Article | 1 October 1999 Paper

Integration of a microlens on a micro XY-stage

Che-Heung Kim, Yong-Kweon Kim

Proceedings Volume 3892, (1999) https://doi.org/10.1117/12.364508

KEYWORDS: Actuators, Fresnel lenses, Spherical lenses, Microlens, Glasses, Fabrication, Silicon, Control systems, Diffraction, Photoresist materials

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Proceedings Article | 29 September 1999 Paper

Fabrication of vertical-spring-type micromirror with SiO2 shielding screen and composite-layered mirror plate

TaeSun Lim, Yong-Kweon Kim

Proceedings Volume 3891, (1999) https://doi.org/10.1117/12.364447

KEYWORDS: Mirrors, Aluminum, Micromirrors, Photoresist materials, Reactive ion etching, Composites, Optical lithography, Silica, Dry etching, Heat treatments

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Proceedings Article | 1 June 1999 Paper

Fabrication and deflection measurement of micromirrors supported by an S-shape girder

Hoseong Kim, Jonggook Kim, Yong-Kweon Kim, Hyungjae Shin

Proceedings Volume 3633, (1999) https://doi.org/10.1117/12.349317

KEYWORDS: Micromirrors, Mirrors, Electrodes, Aluminum, Objectives, Calibration, Laser systems engineering, Electronics, Helium neon lasers, Silicon

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Proceedings Article | 10 September 1998 Paper

Design and fabrication of variable focusing lens

Si-Hong Ahn, Yong-Kweon Kim

Proceedings Volume 3515, (1998) https://doi.org/10.1117/12.322093

KEYWORDS: Glasses, Silicon, Etching, Microfluidics, Lens design, Optical components, Chemical mechanical planarization, Anisotropic etching, Microscopes, Refractive index

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Proceedings Article | 2 September 1998 Paper

Design and fabrication of hidden-spring-structure-type micro-SLM for phase and amplitude modulation

Seok-Whan Chung, Yong-Kweon Kim

Proceedings Volume 3513, (1998) https://doi.org/10.1117/12.324274

KEYWORDS: Electrodes, Spatial light modulators, Mirrors, Amplitude modulation, Aluminum, Photoresist materials, Etching, Phase modulation, Phase shift keying, Electromechanical design

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Proceedings Article | 2 September 1998 Paper

Design and fabrication of micromirror array with hidden joint structures

Chang-Hyeon Ji, Yong-Kweon Kim

Proceedings Volume 3513, (1998) https://doi.org/10.1117/12.324284

KEYWORDS: Micromirrors, Mirrors, Electrodes, Structural design, Mirror structures, Spatial light modulators, Modulation, Metals, Photoresist materials, Oxygen

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Proceedings Article | 2 September 1998 Paper

Modeling and measurement of electrostatic micromirror array fabricated with single-layer polysilicon micromachining technology

Young-Hoon Min, Yong-Kweon Kim

Proceedings Volume 3513, (1998) https://doi.org/10.1117/12.324283

KEYWORDS: Mirrors, Micromirrors, Micromachining, Optical correlators, Actuators, Phase modulation, Silicon, Adaptive optics, Optical fabrication, Optical arrays

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Proceedings Article | 31 August 1998 Paper

Novel fabrication of comb actuator using RIE of polysilicon and (110) Si anisotropic bulk etching in KOH

Hyung-Taek Lim, Yong-Kweon Kim

Proceedings Volume 3511, (1998) https://doi.org/10.1117/12.324297

KEYWORDS: Etching, Silicon, Actuators, Electrodes, Anisotropic etching, Semiconducting wafers, Reactive ion etching, Wet etching, Photomasks, Fabrication

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Proceedings Article | 31 August 1998 Paper

Fabrication of freestanding microstructures using UV lithography and double-electroplating technique

Chang-Wook Baek, Yong-Kweon Kim

Proceedings Volume 3511, (1998) https://doi.org/10.1117/12.324305

KEYWORDS: Plating, Electrodes, Electroplating, Nickel, Gold, Lithography, Surface micromachining, Photoresist materials, Actuators, Ultraviolet radiation

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Proceedings Article | 15 March 1998 Paper

Automatic test equipment for the micromirror array

Hoseong Kim, Kwangwoo Cho, Yong-Kweon Kim, Jong-Woo Shin, Hyungjae Shin, Jae-Ho Moon

Proceedings Volume 3276, (1998) https://doi.org/10.1117/12.302387

KEYWORDS: Micromirrors, Mirrors, Signal processing, Video processing, Photodiodes, Reflectivity, CCD cameras, Video, Electronics, Aluminum

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Proceedings Article | 31 March 1997 Paper

Development of biocompatible micropatterns for single-cell biosensors

Jun Keun Chang, Chan Young Park, Jung-Hoon Choi, Yong-Kweon Kim, Dong-Chul Han

Proceedings Volume 2978, (1997) https://doi.org/10.1117/12.269977

KEYWORDS: Glasses, Proteins, Silicon, Biosensors, Veins, Etching, Cytoskeletons, Photomasks, Coating, Polymerization

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Proceedings Article | 13 September 1995 Paper

Fabrication of micromirror supported by electroplated nickel posts

Jong-Woo Shin, Seok-Whan Chung, Yong-Kweon Kim, Eun-Ho Lee, Beom-Gyu Choi, Se-Jin Ahn

Proceedings Volume 2641, (1995) https://doi.org/10.1117/12.220928

KEYWORDS: Mirrors, Nickel, Micromirrors, Aluminum, Electrodes, Photoresist materials, Electroplating, Silicon, Reactive ion etching, Etching

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Showing 5 of 20 publications

Conference Committee Involvement (1)

MEMS/MOEMS Technologies and Applications II

10 November 2004 | Beijing, China

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