Dr. William C. Warger Profile

Dr. William C. Warger

at Thorlabs Inc

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Author

Publications (11)

SPIE Journal Paper | 12 December 2012 Open Access

Validation of two-dimensional and three-dimensional measurements of subpleural alveolar size parameters by optical coherence tomography

Carolin Unglert, William Warger, Jeroen Hostens, Eman Namati, Reginald Birngruber, Brett Bouma, Guillermo Tearney

JBO, Vol. 17, Issue 12, 126015, (December 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.12.126015

KEYWORDS: Optical coherence tomography, Tissues, Image segmentation, Refractive index, Lung, Refraction, 3D modeling, In vivo imaging, Error analysis, 3D metrology

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SPIE Journal Paper | 11 June 2012 Open Access

Evaluation of optical reflectance techniques for imaging of alveolar structure

Carolin Unglert, Eman Namati, William Warger, Linbo Liu, Hongki Yoo, DongKyun Kang, Brett Bouma, Guillermo Tearney

JBO, Vol. 17, Issue 7, 071303, (June 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.7.071303

KEYWORDS: Lung, Tissues, Reflectivity, Imaging systems, Tissue optics, Image resolution, Visualization, In vivo imaging, Biomedical optics, Confocal microscopy

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Proceedings Article | 4 March 2009 Paper

Accurate phase measurements for thick spherical objects using optical quadrature microscopy

William Warger, Charles DiMarzio

Proceedings Volume 7184, 71840G (2009) https://doi.org/10.1117/12.809545

KEYWORDS: Ray tracing, Refractive index, Polymethylmethacrylate, Phase measurement, Microscopy, Optical spheres, Spherical lenses, Optical microscopy, Digital image correlation, Microscopes

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Proceedings Article | 4 March 2009 Paper

Comparison of optical quadrature microscopy and Shack-Hartmann wavefront sensor

Yogesh Patel, William Warger, Jerome Ballesta, Charles DiMarzio

Proceedings Volume 7184, 71840F (2009) https://doi.org/10.1117/12.810105

KEYWORDS: Wavefront sensors, Polymethylmethacrylate, Microscopy, Beam splitters, Wavefronts, Biomedical optics, Image processing, Optical microscopy, Sensors, Cameras

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Several quantitative phase imaging techniques, such as digital holography, Hilbert-phase microscopy, and phase-shifting interferometry have applications in biological and medical imaging. Quantitative phase imaging measures the changes in the wavefront of the incident light due to refractive index variations throughout a 3-D specimen. We have developed a multimodal microscope which combines optical quadrature microscopy (OQM) and a Shack- Hartmann wavefront sensor for applications in biological imaging. OQM is an interferometric imaging modality that noninvasively measures the amplitude and phase of a signal beam that travels through a transparent specimen. The phase is obtained from interferograms with four different delayed reference wavefronts. The phase is then transformed into a quantitative image of optical path length difference. The Shack-Hartmann wavefront sensor measures the gradient of the wavefront at various points across a beam. A microlens array focuses the local wavefront onto a specific region of the CCD camera. The intensity is given by the maximum amplitude in the region and the phase is determined based on the exact pixel position within the region. We compare the amplitude and quantitative phase information of poly-methyl-meth-acrylate (PMMA) beads in oil and one-cell and two-cell mouse embryos with micrometer resolution using OQM and the Shack-Hartmann. Each pixel in OQM provides a phase measurement, whereas multiple pixels are used in Shack-Hartmann to determine the tilt. Therefore, the simple Shack-Hartmann system is limited by its resolution and field-of-view. Real-time imaging in Shack-Hartmann requires spatial averaging which smoothes the edges of the PMMA beads. The OQM has a greater field-of-view with good resolution; however, it is a complex system requiring multiple optical components and four cameras which may introduce additional artifacts in processing quantitative images. The OQM and Shack- Hartmann has certain advantages depending on the application. A combination of these two systems may provide improved quantitative phase information than either one alone.cHJl

Proceedings Article | 4 March 2009 Paper

Efficient confocal microscopy with a dual-wedge scanner

William Warger, Stephen Guerrera, Zachary Eastman, Charles DiMarzio

Proceedings Volume 7184, 71840M (2009) https://doi.org/10.1117/12.810527

KEYWORDS: Prisms, Scanners, Confocal microscopy, Microscopes, Objectives, Polygon scanners, Skin, Raster graphics, Telescopes, Reflectivity

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SPIE Journal Paper | 1 May 2008 Open Access

Phase-subtraction cell-counting method for live mouse embryos beyond the eight-cell stage

William Warger, Judith Newmark, Carol Warner, Charles DiMarzio

JBO, Vol. 13, Issue 03, 034005, (May 2008) https://doi.org/10.1117/12.10.1117/1.2937468

KEYWORDS: Digital image correlation, Microscopy, Imaging systems, Luminescence, Refraction, Biomedical optics, Confocal microscopy, Sensing systems, In vitro testing, Optical microscopy

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Proceedings Article | 12 February 2008 Paper

Modeling of optical quadrature microscopy for imaging mouse embryos

William Warger, Charles DiMarzio

Proceedings Volume 6861, 68610T (2008) https://doi.org/10.1117/12.763916

KEYWORDS: Beam splitters, Microscopy, Interference (communication), Optical microscopy, Cameras, CCD cameras, 3D image reconstruction, Wavefronts, Microscopes, Error analysis

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SPIE Journal Paper | 1 July 2007 Open Access

Multimodal optical microscope for detecting viability of mouse embryos in vitro

William Warger, Gary Laevsky, Daniel Townsend, Milind Rajadhyaksha, Charles DiMarzio

JBO, Vol. 12, Issue 04, 044006, (July 2007) https://doi.org/10.1117/12.10.1117/1.2753744

KEYWORDS: Microscopes, Confocal microscopy, Mirrors, Microscopy, Beam splitters, Digital image correlation, Objectives, Optical microscopes, Scanners, Interfaces

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Proceedings Article | 14 February 2007 Paper

Toward a compact dual-wedge point-scanning confocal reflectance microscope

William Warger, Stephen Guerrera, Charles DiMarzio

Proceedings Volume 6443, 644311 (2007) https://doi.org/10.1117/12.701016

KEYWORDS: Prisms, Confocal microscopy, Microscopes, Scanners, Reflectivity, Objectives, 3D scanning, Laser scanners, Telescopes, Sensors

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Proceedings Article | 23 February 2006 Paper

Accurate cell counts in live mouse embryos using optical quadrature and differential interference contrast microscopy

William Warger, Judith Newmark, Bing Zhao, Carol Warner, Charles DiMarzio

Proceedings Volume 6090, 609009 (2006) https://doi.org/10.1117/12.644922

KEYWORDS: Digital image correlation, Microscopy, Beam splitters, Optical microscopy, Image analysis, Image processing, Neodymium, Polarizers, Biomedical optics, In vitro testing

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Proceedings Article | 29 March 2005 Paper

Combining optical quadrature and differential interference contrast to facilitate embryonic cell counting with fluorescence imaging for confirmation

William Warger, Judith Newmark, ChihChing Chang, Dana Brooks, Carol Warner, Charles DiMarzio

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.591249

KEYWORDS: Digital image correlation, Cameras, Luminescence, Microscopes, Beam splitters, Microscopy, Algorithm development, Interferometers, Optical microscopy, Signal detection

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Showing 5 of 11 publications

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