Dr. Cai Li Profile

Dr. Cai Li

Product Development Engineer

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Publications (10)

SPIE Journal Paper | 16 February 2023 Open Access

Multimodal fluorescence lifetime imaging and optical coherence tomography for longitudinal monitoring of tissue-engineered cartilage maturation in a preclinical implantation model

Xiangnan Zhou, Anne Haudenschild, Cai Li, Laura Marcu

JBO, Vol. 28, Issue 02, 026003, (February 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.2.026003

KEYWORDS: Tissues, Optical coherence tomography, Cartilage, Biological samples, In vivo imaging, Fluorescence, Quantum channels, In vitro testing, Fluorescence lifetime imaging, Nondestructive evaluation

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SignificanceCartilage tissue engineering is a promising strategy for effective curative therapies for treatment of osteoarthritis. However, tissue engineers depend predominantly on time-consuming, expensive, and destructive techniques as quality control to monitor the maturation of engineered cartilage. This practice can be impractical for large-scale biomanufacturing and prevents spatial and temporal monitoring of tissue growth, which is critical for the fabrication of clinically relevant-sized cartilage constructs. Nondestructive multimodal imaging techniques combining fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) hold great potential to address this challenge.AimThe feasibility of using multimodal FLIm–OCT for nondestructive, spatial, and temporal monitoring of self-assembled cartilage tissue maturation in a preclinical mouse model is investigated.ApproachSelf-assembled cartilage constructs were developed for 4 weeks in vitro followed by 4 weeks of in vivo maturation in nude mice. Sterile and nondestructive in situ multispectral FLIm and OCT imaging were carried out at multiple time points (t = 2, 4, and 8 weeks) during tissue development. FLIm and 3D volumetric OCT images were reconstructed and used for the analysis of tissue biochemical homogeneity, morphology, and structural integrity. A biochemical homogeneity index was computed to characterize nonhomogeneous tissue growth at different time points. OCT images were validated against histology.ResultsFLIm detects heterogenous extracellular matrix (ECM) growth of tissue-engineered cartilage. The outer edge of the tissue construct exhibited longer fluorescence lifetime in 375 to 410 and 450 to 485 nm spectral channels, indicating increase in collagen content. Significant (p < 0.05) decrease of construct homogeneity index was observed between t = 2 weeks and t = 4 weeks. Both FLIm and OCT images revealed defects (voids) at the center of the tissue construct during in vitro culture (t = 2 and 4 weeks). Cyst formation during in vivo culture was detected by OCT and confirmed with histology.ConclusionsThe ability of multimodal FLIm–OCT to nondestructively monitor the heterogenous growth of engineered tissue constructs in situ is demonstrated. Spatial and temporal variation of construct ECM component was detected by FLIm. OCT reveals structural defects (voids and cysts). This multimodal approach has great potential to replace costly destructive tests in the manufacturing of tissue-engineered medical products, facilitating their clinical translation.

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SPIE Journal Paper | 21 July 2022 Open Access

Dual-modality fluorescence lifetime imaging-optical coherence tomography intravascular catheter system with freeform catheter optics

Cai Li, Julien Bec, Xiangnan Zhou, Laura Marcu

JBO, Vol. 27, Issue 07, 076005, (July 2022) https://doi.org/10.1117/12.10.1117/1.JBO.27.7.076005

KEYWORDS: Optical coherence tomography, Luminescence, Ultraviolet radiation, Freeform optics, Infrared imaging, Imaging systems, Collimators, Tomography, Arteries, UV optics

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Proceedings Article | 7 March 2022 Presentation

Evaluation of a high-performance FLIm/OCT catheter system for biochemical and structural evaluation of atherosclerosis

Julien Bec, Cai Li, Xiangnan Zhou, Deborah Vela, Laura Marcu

Proceedings Volume PC11936, PC1193604 (2022) https://doi.org/10.1117/12.2607844

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Proceedings Article | 5 March 2021 Presentation

Assessment of murine gut dysbiosis with intraluminal fluorescence lifetime imaging

Alba Alfonso García, Stephanie Cevallos, Jee-Yon Lee, Cai Li, Julien Bec, Andreas Bäumler, Laura Marcu

Proceedings Volume 11626, 116260I (2021) https://doi.org/10.1117/12.2578687

KEYWORDS: Tissues, Fluorescence lifetime imaging, Colon, Mode conditioning cables, Luminescence, Switches, Sodium, Inflammation, In vivo imaging, Diagnostics

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Proceedings Article | 5 March 2021 Presentation

High performance FLIm/OCT catheter system compatible for biochemical/structural evaluation of atherosclerosis

Cai Li, Julien Bec, Xiangnan Zhou, Laura Marcu

Proceedings Volume 11621, 116210P (2021) https://doi.org/10.1117/12.2577322

KEYWORDS: Optical coherence tomography, Solid state electronics, Integrated optics, Collimators, Tissues, Spectroscopy, Sensors, Microchannel plates, Light emitting diodes

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Proceedings Article | 9 March 2020 Presentation

Novel broadband freeform micro-optics for multimodal FLIm-OCT intravascular catheter (Conference Presentation)

Julien Bec, Cai Li, Laura Marcu

Proceedings Volume 11215, 112150H (2020) https://doi.org/10.1117/12.2544488

KEYWORDS: Micro optics, Optical coherence tomography, UV optics, GRIN lenses, Near ultraviolet, Optics manufacturing, Fluorescence lifetime imaging, Tissues, Chromatic aberrations, Reflectivity

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Proceedings Article | 9 March 2020 Presentation

Fiber-based instrument for simultaneous exogenous fluorescence and endogenous fluorescence lifetime imaging of engineered vascular tissue (Conference Presentation)

Cai Li, Alba Alfonso García, Lauren Uyesaka, Benjamin Sherlock, Leigh Griffiths, Laura Marcu

Proceedings Volume 11243, 1124313 (2020) https://doi.org/10.1117/12.2545251

KEYWORDS: Luminescence, Tissues, Imaging systems, Fluorescence lifetime imaging, Microscopy, Visualization, Optical imaging, Tissue optics, Multiplexing, Reflectivity

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Proceedings Article | 7 March 2019 Presentation

Study of vascular tissue grafts cellularization process in bioreactors using label-free fluorescence lifetime imaging (Conference Presentation)

Cai Li, James Mcmasters, Alba Alfonso García, Julien Bec, Anne Haudenschild, Alyssa Panitch, Leigh Griffiths, Laura Marcu

Proceedings Volume 10855, 108550A (2019) https://doi.org/10.1117/12.2510823

KEYWORDS: Tissues, Fluorescence lifetime imaging, Nondestructive evaluation, Control systems, Manufacturing, Multispectral imaging, Stem cells, Prototyping

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Proceedings Article | 4 March 2019 Presentation

Multimodal evaluation of tissue engineered cartilage maturation in a pre-clinical implantation model (Conference Presentation)

Anne Haudenschild, Xiangnan Zhou, Cai Li, Jerry Hu, J. Kent Leach, Kyriacos Athanasiou, Laura Marcu

Proceedings Volume 10876, 108760A (2019) https://doi.org/10.1117/12.2509047

KEYWORDS: Tissues, Cartilage, Optical coherence tomography, Nondestructive evaluation, Tissue optics, Optics manufacturing, Imaging systems, Fluorescence lifetime imaging, Fiber optics, Interfaces

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Proceedings Article | 14 March 2018 Presentation

Study of bovine pericardium biochemical and biomechanical properties during collagenase degradation using fluorescence lifetime imaging (Conference Presentation)

Cai Li, Jeny Shklover, Mojtaba Parvizi, Benjamin Sherlock, Alba Alfonso García, Leigh Griffiths, Laura Marcu

Proceedings Volume 10471, 1047103 (2018) https://doi.org/10.1117/12.2287487

KEYWORDS: Collagen, Fluorescence lifetime imaging, In vivo imaging, Luminescence, Proteins, Molecules, Tissue engineering, Nondestructive evaluation, In vitro testing, Imaging systems

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Showing 5 of 10 publications

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