Dr. Guillermo L. Monroy Profile

Dr. Guillermo L. Monroy

Postdoctoral Researcher at Beckman Institute for Advanced Science and Technology

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Publications (25)

Proceedings Article | 13 March 2024 Presentation

Presentation

Prognostic value of optical coherence tomography (OCT) with machine learning for assessing clinical outcomes of otitis media

Alexander Ho, Qian Jiang, Katherine Peterson, Roxanne Link, Guillermo Monroy, Darold Spillman, Edita Aksamitiene, Joseph Kerschner, Stephen Boppart, Minh Do

Proceedings Volume PC12818, (2024) https://doi.org/10.1117/12.3003143

KEYWORDS: Optical coherence tomography, Machine learning, Biofilms, Visual process modeling, Tissues, Diseases and disorders, Visualization, Video, Scattering, Image resolution

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Proceedings Article | 13 March 2024 Presentation

Presentation

Robust supercontinuum generation using an yttrium aluminum garnet crystal for multimodal label-free multiphoton microscopy

Alejandro De la Cadena, Jaena Park, Kayvan Forouhesh Tehrani, Carlos Renteria, Guillermo Monroy, Stephen Boppart

Proceedings Volume PC12854, PC128540N (2024) https://doi.org/10.1117/12.3002654

KEYWORDS: YAG lasers, Supercontinuum generation, Multiphoton microscopy, Crystals, Nonlinear crystals, Microscopes, Tissues, Photonic crystals, Laser radiation, Radiotherapy

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Proceedings Article | 13 March 2024 Presentation

Presentation

Imaging middle ear tissues with biofilms and cholesteatoma using polarization-sensitive swept-source optical coherence tomography

Adam Markowicz, Rishyashring Iyer, Kavya Sudhir, Guillermo Monroy, Darold Spillman, Stephen Boppart

Proceedings Volume PC12818, (2024) https://doi.org/10.1117/12.3001909

KEYWORDS: Biofilms, Ear, Tissues, Optical coherence tomography, Pathology, Birefringence, Portability, Polarization, In vivo imaging, In vitro testing

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SPIE Journal Paper | 17 April 2023 Open Access

Open Access

Development of polarization-sensitive optical coherence tomography imaging platform and metrics to quantify electrostimulation-induced peripheral nerve injury in vivo in a small animal model

Guillermo Monroy, Mohsen Erfanzadeh, Michael Tao, Damon DePaoli, Ilyas Saytashev, Ahhyun Stephanie Nam, Harmain Rafi, Kasey Kwong, Katherine Shea, Benjamin Vakoc, Srikanth Vasudevan, Daniel Hammer

NPh, Vol. 10, Issue 02, 025004, (April 2023) https://doi.org/10.1117/12.10.1117/1.NPh.10.2.025004

KEYWORDS: Nerve, Optical coherence tomography, Coherence imaging, Animals, Nervous system, Injuries, Biological imaging, Optical imaging, Image segmentation, Tissue optics

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SignificanceNeuromodulation devices are rapidly evolving for the treatment of neurological diseases and conditions. Injury from implantation or long-term use without obvious functional losses is often only detectable through terminal histology. New technologies are needed that assess the peripheral nervous system (PNS) under normal and diseased or injured conditions.AimWe aim to demonstrate an imaging and stimulation platform that can elucidate the biological mechanisms and impacts of neurostimulation in the PNS and apply it to the sciatic nerve to extract imaging metrics indicating electrical overstimulation.ApproachA sciatic nerve injury model in a 15-rat cohort was observed using a newly developed imaging and stimulation platform that can detect electrical overstimulation effects with polarization-sensitive optical coherence tomography. The sciatic nerve was electrically stimulated using a custom-developed nerve holder with embedded electrodes for 1 h, followed by a 1-h recovery period, delivered at above-threshold Shannon model k-values in experimental groups: sham control (SC, n = 5, 0.0 mA / 0 Hz), stimulation level 1 (SL1, n = 5, 3.4 mA / 50 Hz, and k = 2.57), and stimulation level 2 (SL2, n = 5, 6.8 mA / 100 Hz, and k = 3.17).ResultsThe stimulation and imaging system successfully captured study data across the cohort. When compared to a SC after a 1-week recovery, the fascicle closest to the stimulation lead showed an average change of +4 % / − 309 % (SL1/SL2) in phase retardation and −79 % / − 148 % in optical attenuation relative to SC. Analysis of immunohistochemistry (IHC) shows a +1 % / − 36 % difference in myelin pixel counts and −13 % / + 29 % difference in axon pixel counts, and an overall increase in cell nuclei pixel count of +20 % / + 35 % . These metrics were consistent with IHC and hematoxylin/eosin tissue section analysis.ConclusionsThe poststimulation changes observed in our study are manifestations of nerve injury and repair, specifically degeneration and angiogenesis. Optical imaging metrics quantify these processes and may help evaluate the safety and efficacy of neuromodulation devices.

Proceedings Article | 17 March 2023 Presentation

Presentation

Imaging and assessment of human ear wax with optical coherence tomography

Alexander Ho, Guillermo Monroy, Edita Aksamitiene, Darold Spillman, Marina Marjanovic, Stephen Boppart

Proceedings Volume 12354, 1235402 (2023) https://doi.org/10.1117/12.2649235

KEYWORDS: Optical coherence tomography, Ear, Image segmentation, Signal attenuation, Proteins, Product engineering, Medicine, Imaging systems, Human subjects, Fungi

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Proceedings Article | 17 March 2023 Presentation

Presentation

Integrated Raman spectroscopy and optical coherence tomography system for characterizing otitis media

Guillermo Monroy, Sean Fitzgerald, Andrea Locke, Jungeun Won, Darold Spillman, Alexander Ho, Farzana Zaki, Honggu Choi, Eric Chaney, Jay Werkhaven, Kevin Mason, Anita Mahadevan-Jansen, Stephen Boppart

Proceedings Volume 12354, 1235404 (2023) https://doi.org/10.1117/12.2648504

KEYWORDS: Optical coherence tomography, Ear, Raman spectroscopy, Remote sensing, Visualization, Prototyping, In vitro testing, Human subjects, Diagnostics, Bone

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Proceedings Article | 17 March 2023 Presentation

Presentation

Microplasma exposure as a novel therapeutic treatment for otitis media demonstrated in a pre-clinical chinchilla model

Guillermo Monroy, Jinhong Kim, Alexander Ho, Andrey Mironov, Farzana Zaki, Darold Spillman, Gabrielle Choo-Kang, Jungeun Won, Peter Sun, Edita Aksamitiene, Marina Marjanovic, Eric Chaney, Thanh Huong Nguyen, J. Gary Eden, Stephen Boppart

Proceedings Volume PC12358, PC1235809 (2023) https://doi.org/10.1117/12.2648469

KEYWORDS: Ear, Chemical mechanical planarization, Optical coherence tomography, In vitro testing, Injuries, Animal model studies, Tissues, Resistance, Medicine, Instrument modeling

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Proceedings Article | 17 March 2023 Presentation

Presentation

Speciation of otitis media causing bacterial biofilms using texture analysis features from OCT images

Farzana Zaki, Kavya Sudhir, Guillermo Monroy, Jindou Shi, Stephen Boppart

Proceedings Volume 12354, 1235403 (2023) https://doi.org/10.1117/12.2647878

KEYWORDS: Optical coherence tomography, Biological research, Ear, In vitro testing, Image analysis, Principal component analysis, In vivo imaging, Feature extraction, Binary data

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Proceedings Article | 7 March 2022 Presentation

Presentation

Non-invasive detection and characterization of otitis media causing bacteria and bacterial biofilms through Raman spectroscopy and optical coherence tomography

Farzana Zaki, Andrea Locke, Sean Fitzgerald, Kavya Sudhir, Guillermo Monroy, Honggu Choi, Jungeun Won, Stephen Boppart, Anita Mahadevan-Jansen

Proceedings Volume PC11935, PC1193502 (2022) https://doi.org/10.1117/12.2608410

KEYWORDS: Optical coherence tomography, Raman spectroscopy, Bacteria, Ear, Remote sensing, Diagnostics, Spectroscopy, Resistance, Refractive index, Real time imaging

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Proceedings Article | 2 March 2022 Presentation

Presentation

Doppler shift induced by dynamic light scattering of biofilm morphology transition

Honggu Choi, Farzana Zaki, Guillermo Monroy, Jungeun Won, Stephen Boppart

Proceedings Volume PC11959, PC1195905 (2022) https://doi.org/10.1117/12.2609802

KEYWORDS: Doppler tomography, Dynamic light scattering, Diffusion, Biological research, Structural dynamics, Optical coherence tomography, Interfaces

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Proceedings Article | 5 March 2021 Presentation

Presentation

Inactivation and sensitization of middle ear infection related bacteria and biofilm by microplasma jet array

Jungeun Won, Peter Sun, Gabrielle Choo-Kang, Shouyan Li, Wenyuan Chen, Guillermo Monroy, Eric Chaney, J. Eden, Thanh Nguyen, Stephen Boppart

Proceedings Volume 11626, 116260K (2021) https://doi.org/10.1117/12.2577424

KEYWORDS: Ear, Bacteria, Plasmas, Resistance, Plasma treatment

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Proceedings Article | 5 March 2021 Presentation

Presentation

In vivo optical characterization of middle ear effusions associated with otitis media

Jungeun Won, Guillermo Monroy, Ryan Porter, Michael Novak, Ronit Barkalifa, Darold Spillman, Edita Aksamitiene, Eric Chaney, Stephen Boppart

Proceedings Volume 11627, 116270A (2021) https://doi.org/10.1117/12.2577278

KEYWORDS: Ear, Optical coherence tomography, In vivo imaging, Surgery, Visualization, Testing and analysis, Scattering, Proteins, Diagnostics, Biomedical optics

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Proceedings Article | 7 March 2019 Presentation

Presentation

Detecting the efficacy of endotracheal tube suctioning in intubated ICU patients using in vivo catheter-based OCT (Conference Presentation)

Roshan Dsouza, Darold Spillman, Ronit Barkalifa, Guillermo Monroy, Eric Chaney, Karen White, Stephen Boppart

Proceedings Volume 10853, 108530C (2019) https://doi.org/10.1117/12.2508022

KEYWORDS: Optical coherence tomography, In vivo imaging, Bacteria, Image analysis, Pathogens, Resistance, 3D image processing, Stereoscopy, Human subjects, Luminescence

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Proceedings Article | 7 March 2019 Presentation

Presentation

Compact low-cost briefcase OCT system with automated classification for point-of-care diagnosis of otitis media (Conference Presentation)

Roshan Dsouza, Jungeun Won, Guillermo Monroy, Darold Spillman, Stephen Boppart

Proceedings Volume 10869, 1086908 (2019) https://doi.org/10.1117/12.2508010

KEYWORDS: Optical coherence tomography, Point-of-care devices, Classification systems, Diagnostics, Ear, Computing systems, Video

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SPIE Journal Paper | 24 September 2018 Open Access

Open Access

Economical and compact briefcase spectral-domain optical coherence tomography system for primary care and point-of-care applications

Roshan Dsouza, Jungeun Won, Guillermo Monroy, Darold Spillman, Stephen Boppart

JBO, Vol. 23, Issue 09, 096003, (September 2018) https://doi.org/10.1117/12.10.1117/1.JBO.23.9.096003

KEYWORDS: Optical coherence tomography, Imaging systems, Spectroscopy, In vivo imaging, Point-of-care devices, Skin, Mirrors, Sensors, CCD cameras, Image registration

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SPIE Journal Paper | 23 December 2017 Open Access

Open Access

Noninvasive in vivo optical coherence tomography tracking of chronic otitis media in pediatric subjects after surgical intervention

Guillermo Monroy, Paritosh Pande, Ryan Nolan, Ryan Shelton, Ryan Porter, Michael Novak, Darold Spillman, Eric Chaney, Daniel McCormick, Stephen Boppart

JBO, Vol. 22, Issue 12, 121614, (December 2017) https://doi.org/10.1117/12.10.1117/1.JBO.22.12.121614

KEYWORDS: Optical coherence tomography, Surgery, Ear, Bragg cells, In vivo imaging, Visualization, Optical tracking, Imaging systems, Diagnostics, Image analysis

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SPIE Journal Paper | 19 December 2017 Open Access

Open Access

Clinical translation of handheld optical coherence tomography: practical considerations and recent advancements

Guillermo Monroy, Jungeun Won, Darold Spillman, Roshan Dsouza, Stephen Boppart

JBO, Vol. 22, Issue 12, 121715, (December 2017) https://doi.org/10.1117/12.10.1117/1.JBO.22.12.121715

KEYWORDS: Optical coherence tomography, Imaging systems, Tissues, Computer aided design, Ear, In vivo imaging, Surgery, Retina, Visualization, Tissue optics

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SPIE Journal Paper | 31 October 2016 Open Access

Open Access

Rapid diagnosis and differentiation of microbial pathogens in otitis media with a combined Raman spectroscopy and low-coherence interferometry probe: toward in vivo implementation

Youbo Zhao, Guillermo Monroy, Sixian You, Ryan Shelton, Ryan Nolan, Haohua Tu, Eric Chaney, Stephen Boppart

JBO, Vol. 21, Issue 10, 107005, (October 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.10.107005

KEYWORDS: Raman spectroscopy, Ear, Pathogens, Remote sensing, In vivo imaging, Surface plasmons, Tissues, Microorganisms, Spectroscopy, Bacteria

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Proceedings Article | 27 April 2016 Presentation

Presentation

Differentiation of bacterial versus viral otitis media using a combined Raman scattering spectroscopy and low coherence interferometry probe (Conference Presentation)

Youbo Zhao, Ryan Shelton, Haohua Tu, Ryan Nolan, Guillermo Monroy, Eric Chaney, Stephen Boppart

Proceedings Volume 9689, 96892E (2016) https://doi.org/10.1117/12.2211824

KEYWORDS: Raman spectroscopy, Raman scattering, Interferometry, Spectroscopy, Molecular spectroscopy, Diagnostics, Resistance, Spectroscopes, Viruses, Bacteria

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Proceedings Article | 27 April 2016 Presentation

Presentation

A novel mosaicking algorithm for in vivo full-field thickness mapping of the human tympanic membrane using low coherence interferometry (Conference Presentation)

Paritosh Pande, Ryan Shelton, Guillermo Monroy, Ryan Nolan, Stephen Boppart

Proceedings Volume 9689, 96892C (2016) https://doi.org/10.1117/12.2213636

KEYWORDS: In vivo imaging, Interferometry, Imaging systems, Image registration, Ear, Pathology, Coherence (optics), Video, Biomedical optics, Retinal scanning

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Proceedings Article | 27 April 2016 Presentation

Presentation

Comparison of advanced optical imaging techniques with current otolaryngology diagnostics for improved middle ear assessment (Conference Presentation)

Ryan Nolan, Ryan Shelton, Guillermo Monroy, Darold Spillman, Michael Novak, Stephen Boppart

Proceedings Volume 9689, 96892J (2016) https://doi.org/10.1117/12.2213158

KEYWORDS: Ear, Diagnostics, Optical imaging, Optical coherence tomography, Doppler tomography, Vibrometry, Acoustics, Reflectometry, Standards development, Tissues

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Proceedings Article | 26 April 2016 Presentation

Presentation

Development of a low-cost hand-held system for optical coherence tomography imaging (Conference Presentation)

Paritosh Pande, Ryan Shelton, Guillermo Monroy, Ryan Nolan, Stephen Boppart

Proceedings Volume 9697, 96971D (2016) https://doi.org/10.1117/12.2213660

KEYWORDS: Imaging systems, Optical coherence tomography, Coherence imaging, Optical imaging, Interferometers, Signal to noise ratio, Coherence (optics), Light, Beam splitters, Detector arrays

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Proceedings Article | 28 February 2014 Paper

Paper

Implementation and evaluation of Google Glass for visualizing real-time image and patient data in the primary care office

Guillermo Monroy, Nathan Shemonski, Ryan Shelton, Ryan Nolan, Stephen Boppart

Proceedings Volume 8935, 893514 (2014) https://doi.org/10.1117/12.2040221

KEYWORDS: Glasses, Medicine, Imaging systems, Diagnostics, Optical coherence tomography, Image visualization, Heads up displays, Video, Visualization, Tissues

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Proceedings Article | 27 February 2014 Paper

Paper

In vivo intra-operative breast tumor margin detection using a portable OCT system with a handheld surgical imaging probe

Sarah Erickson-Bhatt, Ryan Nolan, Nathan Shemonski, Steven Adie, Jeffrey Putney, Donald Darga, Daniel McCormick, Andrew Cittadine, Marina Marjanovic, Eric Chaney, Guillermo Monroy, Fredrick South, P. Scott Carney, Kimberly Cradock, Z. George Liu, Partha Ray, Stephen Boppart

Proceedings Volume 8935, 89351C (2014) https://doi.org/10.1117/12.2040315

KEYWORDS: Tumors, Optical coherence tomography, Surgery, In vivo imaging, Imaging systems, Tissues, Breast, Breast cancer, Mammography, Tissue optics

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Proceedings Article | 20 March 2013 Paper

Paper

Interferometric synthetic aperture microscopy implementation on a floating point multi-core digital signal processer

Adeel Ahmad, Murtaza Ali, Fredrick South, Guillermo Monroy, Steven Adie, Nathan Shemonski, P. Scott Carney, Stephen Boppart

Proceedings Volume 8571, 857134 (2013) https://doi.org/10.1117/12.2006876

KEYWORDS: Digital signal processing, Optical coherence tomography, Signal processing, Data processing, Image processing, Interferometry, Synthetic aperture microscopy, Fourier transforms, Infinite impulse response filters, Graphics processing units

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Showing 5 of 25 publications

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