Prof. Peng Xi Profile

Prof. Peng Xi

Professor at Peking Univ

SPIE Involvement:

Conference Program Committee | Editorial Board Member: Advanced Photonics | Author

Websites:

Company Website

Publications (43)

Proceedings Article | 13 March 2024 Presentation

Polar-SIM: super-resolution structured illumination microscopy in XYZλθT dimensions

Peng Xi, He Sun

Proceedings Volume PC12853, PC128530B (2024) https://doi.org/10.1117/12.3008475

KEYWORDS: Super resolution, Light sources and illumination, Modulation, Microscopy, Point spread functions, Super resolution microscopy, Spatial resolution, Polarized microscopy, Neurons, Molecules

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SPIE Journal Paper | 23 December 2023 Open Access

High-speed autopolarization synchronization modulation three-dimensional structured illumination microscopy

Yaning Li, Ruijie Cao, Wei Ren, Yunzhe Fu, Yiwei Hou, Suyi Zhong, Karl Zhanghao, Meiqi Li, Peng Xi

APN, Vol. 3, Issue 01, 016001, (December 2023) https://doi.org/10.1117/12.10.1117/1.APN.3.1.016001

KEYWORDS: Light sources and illumination, Digital micromirror devices, Modulation, Polarization, Microscopy, Imaging systems, Image resolution, Biological imaging, Super resolution, Electrooptic modulation

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SPIE Journal Paper | 11 August 2023 Open Access

Past, Present, and Future: How to Write an Impactful Review

Peng Xi

AP, Vol. 5, Issue 04, 040101, (August 2023) https://doi.org/10.1117/12.10.1117/1.AP.5.4.040101

KEYWORDS: Visualization, Reflection, Photonics, Mixtures, Excel, Chemical elements, Biomedical engineering

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SPIE Journal Paper | 5 December 2022 Open Access

Laterally swept light-sheet microscopy enhanced by pixel reassignment for photon-efficient volumetric imaging

Liang Qiao, Hongjin Li, Suyi Zhong, Xinzhu Xu, Fei Su, Xi Peng, Dayong Jin, Karl Zhanghao

APN, Vol. 2, Issue 01, 016001, (December 2022) https://doi.org/10.1117/12.10.1117/1.APN.2.1.016001

KEYWORDS: Camera shutters, Microscopy, Spatial light modulators, Fluorescence, Confocal microscopy, Gaussian beams, Optical resolution, Image resolution, Cameras, Point spread functions

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Proceedings Article | 27 October 2021 Paper

Polarized structured illumination microscopy

Peng Xi, Karl Zhanghao, Meiqi Li

Proceedings Volume 11925, 1192502 (2021) https://doi.org/10.1117/12.2615382

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Proceedings Article | 10 October 2020 Presentation

Structured illumination microscopy using digital micromirror device and coherent light source

Meiqi Li, Yaning Li, Wenhui Liu, Amit Lal, Shan Jiang, Dayong Jin, Houpu Yang, Shu Wang, Karl Zhanghao, Peng Xi

Proceedings Volume 11553, 1155313 (2020) https://doi.org/10.1117/12.2575154

KEYWORDS: Digital micromirror devices, Microscopy, Micromirrors, Light sources, Spatial light modulators, Spatial resolution, Double patterning technology, Optoelectronics, Switches, Phase shift keying

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Proceedings Article | 20 August 2020 Presentation

Super-resolution microscopy: an adventure on a new dimension

Peng Xi

Proceedings Volume 11468, 1146816 (2020) https://doi.org/10.1117/12.2567259

KEYWORDS: Super resolution, Stimulated emission depletion microscopy, Upconversion, Nanoparticles, Continuous wave operation, Modulation, Bessel beams, Demodulation, Polarization, Microscopy

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SPIE Journal Paper | 2 June 2020 Open Access

Frequency-domain diagonal extension imaging

Shan Jiang, Meiling Guan, Jiamin Wu, Guocheng Fang, Xinzhu Xu, Dayong Jin, Zhen Liu, Kebin Shi, Fan Bai, Shu Wang, Peng Xi

AP, Vol. 2, Issue 03, 036005, (June 2020) https://doi.org/10.1117/12.10.1117/1.AP.2.3.036005

KEYWORDS: Image resolution, Resolution enhancement technologies, Microscopy, Image sensors, Blood, Digital imaging, Optical transfer functions, Photography, Image enhancement, Super resolution

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Proceedings Article | 24 February 2020 Presentation + Paper

GRIN lens: a new element for complex vectorial beam modulation

Chao He, Jianyu Lin, Ben Dai, Peng Xi, Martin Booth

Proceedings Volume 11297, 112970O (2020) https://doi.org/10.1117/12.2545557

KEYWORDS: GRIN lenses, Polarization, Modulation, Birefringence, Optical components, Wave plates, Polarizers, Refractive index

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Proceedings Article | 31 October 2016 Paper

A frequency domain reconstruction of SIM image using four raw images

Amit Lal, XiaoShuai Huang, Peng Xi

Proceedings Volume 10024, 1002411 (2016) https://doi.org/10.1117/12.2246242

KEYWORDS: Reconstruction algorithms, Fourier transforms, Computer simulations, Optical transfer functions, Image resolution, Image enhancement, Resolution enhancement technologies, Image filtering, Image quality, Spatial frequencies

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SPIE Journal Paper | 9 June 2016 Open Access

Superior performance with sCMOS over EMCCD in super-resolution optical fluctuation imaging

Xuanze Chen, Zhiping Zeng, Rongqin Li, Boxin Xue, Peng Xi, Yujie Sun

JBO, Vol. 21, Issue 06, 066007, (June 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.6.066007

KEYWORDS: Electron multiplying charge coupled devices, Signal to noise ratio, Super resolution, Optical imaging, Imaging systems, Biomedical optics, Molecules, Spatial frequencies, Image resolution, Sensors

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Proceedings Article | 28 September 2013 Paper

STED imaging of nitrogen vacancy centers in diamond

Xusan Yang, Yujia Liu, Jinyu Wang, Shaohua Zhang, Hao Xie, Xuanze Chen, Peng Xi

Proceedings Volume 8845, 88450C (2013) https://doi.org/10.1117/12.2023435

KEYWORDS: Stimulated emission depletion microscopy, Diamond, Confocal microscopy, Super resolution microscopy, Luminescence, Imaging systems, Diffraction, Single mode fibers, Biomedical optics

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Proceedings Article | 22 February 2013 Paper

Two-color CW STED nanoscopy

Xuanze Chen, Yujia Liu, Xusan Yang, Tingting Wang, Eric Alonas, Philip Santangelo, Qiushi Ren, Peng Xi

Proceedings Volume 8590, 859017 (2013) https://doi.org/10.1117/12.2001178

KEYWORDS: Stimulated emission depletion microscopy, Super resolution, Proteins, Microscopy, Super resolution microscopy, Point spread functions, Confocal microscopy, Diffraction, Luminescence

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Proceedings Article | 22 February 2013 Paper

Phase relief imaging with confocal laser scanning system

Tong Peng, Hao Xie, Yichen Ding, Peng Xi

Proceedings Volume 8587, 85871M (2013) https://doi.org/10.1117/12.2001181

KEYWORDS: Imaging systems, Confocal microscopy, Objectives, Signal detection, Tissues, Luminescence, Absorption, Microscopy, Laser scanners, Laser systems engineering

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Proceedings Article | 11 December 2012 Paper

LOSOM: phase relief imaging can be achieved with confocal system

Tong Peng, Hao Xie, Yichen Ding, Yiqing Lu, Dayong Jin, Peng Xi

Proceedings Volume 8553, 85531C (2012) https://doi.org/10.1117/12.2000809

KEYWORDS: Imaging systems, Confocal microscopy, Objectives, Signal detection, Microscopy, 3D image processing, Luminescence, Laser optics, Optical microscopy, Visualization

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Proceedings Article | 11 December 2012 Paper

Design of a real-time portable confocal scanning laser microscope

Xusan Yang, Yaohuan Zhao, Gang Yin, Haojie Li, Tingting Wang, Peng Xi

Proceedings Volume 8553, 85531D (2012) https://doi.org/10.1117/12.2001006

KEYWORDS: Confocal microscopy, Mirrors, Microscopes, Video, Control systems, Imaging systems, Laser scanners, Photodiodes, Skin, Clocks

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Proceedings Article | 11 December 2012 Paper

CW STED nanoscopy with a Ti:Sapphire oscillator

Yujia Liu, Hao Xie, Eric Alonas, Philip Santangelo, Dayong Jin, Peng Xi

Proceedings Volume 8553, 85531B (2012) https://doi.org/10.1117/12.2000807

KEYWORDS: Stimulated emission depletion microscopy, Point spread functions, Super resolution, Confocal microscopy, Diffraction, Super resolution microscopy, Microscopy, Oscillators, Multiphoton microscopy

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Fluorescence microscopy has become an essential tool to study biological molecules, pathways and events in living cells, tissues and animals. Meanwhile, the conventional optical microscopy is limited by the wavelength of the light. Even the most advanced confocal microscopy or multiphoton microscopy can only yield optical resolution approaching the diffraction limit of ~200 nm. This is still larger than many subcellular structures, which are too small to be resolved in detail. These limitations have driven the development of super-resolution optical imaging methodologies over the past decade. The stimulated emission depletion (STED) microscopy was the first and most direct approach to overcoming the diffraction limit for far-field nanoscopy. Typically, the excitation focus is overlapped by an intense doughnut-shaped spot to instantly de-excite markers from their fluorescent state to the ground state by stimulated emission. This effectively eliminates the periphery of the Point Spread Function (PSF), resulting in a narrower focal region, or super-resolution. Scanning a sharpened spot through the specimen renders images with sub-diffraction resolution. Multi-color STED imaging can present important structural and functional information for protein-protein interaction. In this work, we presented a dual color, synchronization-free STED stimulated emission depletion (STED) microscopy with a Ti:Sapphire oscillator. The excitation wavelengths were 532nm and 635nm, respectively. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. We also imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the super-resolution resolving power over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED A series of biological specimens were imaged with our dual-color STED.

Proceedings Article | 24 September 2011 Paper

In-vivo full depth of eye imaging spectral domain optical coherence tomography

Cuixia Dai, Chuanqing Zhou, Shuliang Jiao, Peng Xi, Qiushi Ren

Proceedings Volume 8135, 81351I (2011) https://doi.org/10.1117/12.893439

KEYWORDS: Image segmentation, Eye, Optical coherence tomography, In vivo imaging, Spectroscopy, Retina, Mirrors, Cornea, Imaging systems, Multichannel imaging systems

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SPIE Journal Paper | 1 January 2009 Open Access

Two-photon imaging using adaptive phase compensated ultrashort laser pulses

Peng Xi, Yair Andegeko, Dmitry Pestov, Vadim Lozovoy, Marcos Dantus

JBO, Vol. 14, Issue 01, 014002, (January 2009) https://doi.org/10.1117/12.10.1117/1.3059629

KEYWORDS: Pulsed laser operation, Ultrafast phenomena, Second-harmonic generation, Microscopes, Two photon imaging, Objectives, Mirrors, Prisms, Luminescence, Microscopy

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Proceedings Article | 15 February 2008 Paper

Greater signal and contrast in two-photon microscopy with ultrashort pulses

Lindsay Weisel, Peng Xi, Yair Andegeko, Vadim Lovozoy, Marcos Dantus

Proceedings Volume 6860, 68601O (2008) https://doi.org/10.1117/12.763549

KEYWORDS: Dispersion, Two photon excitation microscopy, Ultrafast phenomena, Optical filters, Prisms, Tunable lasers, Biomedical optics, Luminescence, Ultrafast lasers, Femtosecond phenomena

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Proceedings Article | 15 February 2008 Paper

Two-photon laser scanning microscopy with ultrabroad bandwidth 110 nm FWHM femtosecond pulses

Peng Xi, Lindsay Weisel, Yair Andegeko, Vadim Lovozoy, Marcos Dantus

Proceedings Volume 6860, 68601U (2008) https://doi.org/10.1117/12.763698

KEYWORDS: Dispersion, Two photon excitation microscopy, Microscopes, Laser scanners, Luminescence, Microscopy, Femtosecond phenomena, Multiphoton microscopy, Ultrafast phenomena, Objectives

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Proceedings Article | 22 June 2005 Paper

Depth-resolved fluorescence of biological tissue

Yicong Wu, Peng Xi, Tak-Hong Cheung, So Fan Yim, Mei-Yung Yu, Jianan Qu

Proceedings Volume 5771, (2005) https://doi.org/10.1117/12.634626

KEYWORDS: Luminescence, Tissues, Tissue optics, Pathology, Fluorescence spectroscopy, Confocal microscopy, Collagen, Laser tissue interaction, Natural surfaces, Diagnostics

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Proceedings Article | 1 April 2005 Paper

Depth-resolved fluorescence of human ectocervical tissue

Yicong Wu, Peng Xi, Tak-Hong Cheung, So Fan Yim, Mei-Yung Yu, Jianan Qu

Proceedings Volume 5692, (2005) https://doi.org/10.1117/12.593826

KEYWORDS: Luminescence, Tissues, Tissue optics, Collagen, Pathology, Confocal microscopy, Fluorescence spectroscopy, Laser tissue interaction, Natural surfaces, Connective tissue

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Proceedings Article | 21 June 2004 Paper

Fluorescence spectroscopy of biological tissue: single- and two-photon excitation

Yicong Wu, Peng Xi, Weikun Ge, Powing Yuen, Jianan Qu

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.544404

KEYWORDS: Luminescence, Tissues, Fluorescence spectroscopy, Confocal microscopy, Tissue optics, Collagen, Connective tissue, Spectroscopy, Esophagus, Pathology

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Proceedings Article | 12 December 2003 Paper

Symmetric color separation gratings

Changhe Zhou, Yanyan Zhang, Peng Xi, Enwen Dai, Xuming Wu, Liren Liu

Proceedings Volume 5201, (2003) https://doi.org/10.1117/12.504122

KEYWORDS: Diffraction, Diffraction gratings, Optical design, Photomasks, Manufacturing, Tolerancing, Optical alignment, Micro optics, Solids, Refractive index

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Proceedings Article | 6 November 2003 Paper

Fabrication of optical elements with femtosecond doubled-frequency Ti:sapphire laser

Xiaohui Sun, Changhe Zhou, Peng Xi, Enwen Dai, Liren Liu, Bingkun Yu

Proceedings Volume 5174, (2003) https://doi.org/10.1117/12.504016

KEYWORDS: Femtosecond phenomena, Optical components, Photoresist materials, Chromium, Photomasks, Crystals, Sapphire lasers, Laser crystals, Laser systems engineering, Optical filters

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Proceedings Article | 3 November 2003 Paper

Etching quartz with inductively coupled plasma etching equipment

Xuming Wu, Changhe Zhou, Peng Xi, Enwen Dai, Huayi Ru, Liren Liu

Proceedings Volume 5183, (2003) https://doi.org/10.1117/12.504001

KEYWORDS: Etching, Quartz, Plasma, Chemical elements, Argon, Plasma etching, Ions, Photoresist materials, Plasma systems, Polymers

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Proceedings Article | 20 October 2003 Paper

Near-field detection of the quality of high-density gratings with nanotechnology

Peng Xi, Changhe Zhou, Hongxin Luo, Enwen Dai, Liren Liu

Proceedings Volume 5225, (2003) https://doi.org/10.1117/12.503994

KEYWORDS: Diffraction gratings, Near field scanning optical microscopy, Near field, Moire patterns, Nanotechnology, Sensors, Optical microscopes, Photoresist materials, Near field optics, Optical signal processing

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Proceedings Article | 15 November 2002 Paper

Laser beam scanning based on the Talbot phase-encoding method

Changhe Zhou, Peng Xi, Enwen Dai, Liren Liu, Huayi Ru

Proceedings Volume 4788, (2002) https://doi.org/10.1117/12.451627

KEYWORDS: Diffraction, Optical scanning, Scanners, Microlens array, Computer programming, Nonimpact printing, Micro optics, Microelectronics, Glasses, Laser applications

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Proceedings Article | 6 November 2002 Paper

Superresolution technology for small diffraction spot size in the far field

Jia Jia, Changhe Zhou, Wendong Xu, Peng Xi, Liren Liu

Proceedings Volume 4825, (2002) https://doi.org/10.1117/12.451948

KEYWORDS: Super resolution, Diffraction, Binary data, Phase shift keying, Confocal microscopy, Microscopy, Signal detection, Photoresist materials, Reactive ion etching, Wavefronts

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Proceedings Article | 16 September 2002 Paper

Ultrafast study of near-field hexagonal array illumination

Peng Xi, Changhe Zhou, Enwen Dai, Liren Liu

Proceedings Volume 4929, (2002) https://doi.org/10.1117/12.483232

KEYWORDS: Ultrafast phenomena, Near field, Optical communications, Near field optics, Optical computing, Diffraction gratings, Fiber optic illuminators, Fourier transforms, Crystals, Sapphire lasers

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Proceedings Article | 4 September 2002 Paper

Fast and effective algorithm for synthesizing computer-generated holograms

Enwen Dai, Changhe Zhou, Peng Xi, Liren Liu

Proceedings Volume 4768, (2002) https://doi.org/10.1117/12.482190

KEYWORDS: Computer generated holography, Image quality, Optimization (mathematics), Binary data, Reconstruction algorithms, Image enhancement, Glasses, Chromium, Algorithms, Plasma

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Proceedings Article | 30 August 2002 Paper

Space-multiplexed diffractive optical device based on Talbot effect

Enwen Dai, Changhe Zhou, Peng Xi, Liren Liu

Proceedings Volume 4924, (2002) https://doi.org/10.1117/12.481484

KEYWORDS: Fiber optic illuminators, Diffractive optical elements, Optical components, Multiplexing, Binary data, Computer generated holography, Astronomical imaging, Wavefronts, Holograms, Optical interconnects

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Proceedings Article | 26 December 2001 Paper

Pulse-width measurement of ultrashort laser pulse based on Talbot effect

Peng Xi, Changhe Zhou, Shuai Zhao, Enwen Dai, Liren Liu

Proceedings Volume 4438, (2001) https://doi.org/10.1117/12.451484

KEYWORDS: Ultrafast phenomena, Diffraction, Harmonic generation, Physics, Pulsed laser operation, Laser applications, Nonlinear crystals, Numerical analysis, Error analysis, Light sources

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Proceedings Article | 26 December 2001 Paper

Decomposing rule of two-dimensional separable Talbot array illuminator

Shuai Zhao, Changhe Zhou, Peng Xi, Liren Liu

Proceedings Volume 4438, (2001) https://doi.org/10.1117/12.451493

KEYWORDS: Fiber optic illuminators, Physics, Factor analysis, Fabrication

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Proceedings Article | 21 December 2001 Paper

Initial phase assignment method for synthesizing computer generated hologram

Enwen Dai, Changhe Zhou, Peng Xi, Shuai Zhao, Liren Liu

Proceedings Volume 4436, (2001) https://doi.org/10.1117/12.451305

KEYWORDS: Computer generated holography, Binary data, Diffraction, Systems engineering, Holograms, Fourier transforms, Iterative methods, Glasses, Plasma

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Proceedings Article | 6 December 2001 Paper

Phase gratings made with inductively coupled plasma technology

Changhe Zhou, Peng Xi, Enwen Dai, Liren Liu, Huayi Ru

Proceedings Volume 4470, (2001) https://doi.org/10.1117/12.449652

KEYWORDS: Plasma, Reactive ion etching, Process control, Photonic devices, Ions, Etching, Dry etching, Optical components

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Proceedings Article | 6 December 2001 Paper

Possibility of phase-contrast pickup head for two-layered optical disk

Shuai Zhao, Changhe Zhou, Peng Xi, Liren Liu

Proceedings Volume 4470, (2001) https://doi.org/10.1117/12.449663

KEYWORDS: Head, Optical discs, Photonic devices, Objectives

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Proceedings Article | 29 November 2000 Paper

Time dependence of Talbot effect under ultrashort laser illumination

Huaisheng Wang, Changhe Zhou, Shuai Zhao, Peng Xi, Liren Liu

Proceedings Volume 4114, (2000) https://doi.org/10.1117/12.408560

KEYWORDS: Diffraction gratings, Argon, Near field diffraction, Ultrafast phenomena, Femtosecond phenomena, Diffraction, Fourier transforms, Mechanics, Pulsed laser operation, Laser metrology

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Proceedings Article | 29 November 2000 Paper

Arbitrary-phase-modulated Talbot illuminator

Changhe Zhou, Huaisheng Wang, Peng Xi, Shuai Zhao, Liren Liu

Proceedings Volume 4114, (2000) https://doi.org/10.1117/12.408550

KEYWORDS: Fiber optic illuminators, Micro optics, Modulation, Beam splitters, Stars, Optical simulations, Optical discs, Optical components, Phase shift keying, Integrated optics

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Proceedings Article | 27 December 1995 Paper

Fuzzy control method for lateral control of autonomous land vehicle

Hui Wu, Wensen Chang, Han-gen He, Peng Xi

Proceedings Volume 2591, (1995) https://doi.org/10.1117/12.228969

KEYWORDS: Fuzzy logic, Vehicle control, Picosecond phenomena, Phase modulation, Device simulation, Control systems, Nonlinear control, Roads, Mathematical modeling, Sensors

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Proceedings Article | 30 June 1994 Paper

Fuzzy morphology induced by threshold decomposition

Zheng Gao, Han-gen He, Jianping Liu, Xiaolin Liu, Peng Xi

Proceedings Volume 2300, (1994) https://doi.org/10.1117/12.179196

KEYWORDS: Fuzzy logic, Fermium, Binary data, Frequency modulation, Space operations, Image processing, Signal processing, Very large scale integration, Computing systems, Radon

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Proceedings Article | 30 June 1994 Paper

Threshold decomposition of soft-morphological filters into function and set processing (FSP) morphological filters and order-statistic filters

Zheng Gao, Han-gen He, Jianping Liu, Xiaolin Liu, Peng Xi

Proceedings Volume 2300, (1994) https://doi.org/10.1117/12.179197

KEYWORDS: Filtering (signal processing), Binary data, Image filtering, Samarium, Signal processing, Very large scale integration, Digital filtering, Zinc, Digital signal processing, Interference (communication)

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Showing 5 of 43 publications

Conference Committee Involvement (5)

Enhanced Spectroscopies and Nanoimaging 2024

18 August 2024 | San Diego, California, United States

Enhanced Spectroscopies and Nanoimaging 2023

20 August 2023 | San Diego, California, United States

Enhanced Spectroscopies and Nanoimaging 2022

21 August 2022 | San Diego, California, United States

Enhanced Spectroscopies and Nanoimaging 2021

2 August 2021 | San Diego, California, United States

Enhanced Spectroscopies and Nanoimaging 2020

24 August 2020 | Online Only, California, United States

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