Prof. Ki Hean Kim Profile

Prof. Ki Hean Kim

Professor at Pohang Univ of Science and Technology

SPIE Involvement:

Author

Publications (30)

Proceedings Article | 13 March 2024 Presentation

Extended depth-of-field microscopy with surface tracking for noninvasive examination of conjunctival goblet cells in humans

Jungbin Lee, Seonghan Kim, Wan Jae Choi, Jin Suk Ryu, Chang Ho Yoon, Soohyun Park, Won Yeong Park, Noseong Park, Suil Jeon, Jieun Yun, Ki Hean Kim

Proceedings Volume PC12824, PC128240S (2024) https://doi.org/10.1117/12.2687883

KEYWORDS: Microscopy, Depth of field, Human subjects, Diseases and disorders, Tolerancing, Real time imaging, Imaging systems, Image deconvolution, Fluorescence microscopy, Eye models

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Proceedings Article | 26 February 2024 Paper

Open-top two-photon light sheet microscopy for three-dimensional tissue examination

Won Yeong Park, Jieun Yun, Jinho Shin, Byung Ho Oh, Gilsuk Yoon, Seung-Mo Hong, Ki Hean Kim

Proceedings Volume 13076, 1307603 (2024) https://doi.org/10.1117/12.3016904

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Proceedings Article | 17 March 2023 Presentation

Moxifloxacin-based extended depth-of-field (EDOF) wide-field microscopy with surface tracking for non-invasive examination of conjunctival goblet cells in awake human subjects

Ki Hean Kim, Jungbin Lee, Seonghan Kim, Suil Jeon, Jieun Yun, Noseung Park, Kyungbin Bae, Chang Ho Yoon

Proceedings Volume PC12360, PC123600Q (2023) https://doi.org/10.1117/12.2652780

KEYWORDS: Microscopy, Imaging systems, Human subjects, In vivo imaging, Eye, Animal model studies, Visualization, Motion models, Luminescence, Eye models

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Proceedings Article | 15 March 2023 Poster + Paper

Semantic knowledge distillation for conjunctival goblet cell segmentation

Seunghyun Jang, Kyungdeok Seo, Hyunyoung Kang, Seonghan Kim, Seungyoung Kang, Ki Hean Kim, Sejung Yang

Proceedings Volume 12383, 123830I (2023) https://doi.org/10.1117/12.2648170

KEYWORDS: Eye, Image segmentation, Deep learning

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Proceedings Article | 9 March 2022 Presentation

Dual PpIX and moxifloxacin fluorescence confocal imaging for brain tumor delineation

Seonghan Kim, Seunghun Lee, Kihean Kim, Eui Hyun Kim, Kyung Hwa Lee, Chulmin Joo

Proceedings Volume PC11949, PC1194907 (2022) https://doi.org/10.1117/12.2610940

KEYWORDS: Luminescence, Tumors, Confocal microscopy, Neuroimaging, Brain, Surgery, Tissues, Microscopes

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Proceedings Article | 7 March 2022 Presentation

High-contrast visualization of human skin cancers with combined reflectance confocal and moxifloxacin based two-photon microscopy

Won Yeong Park, Bumju Kim, Jihyun Chun, Seung-Mo Hong, Byung Ho Oh, Ki Hean Kim

Proceedings Volume PC11934, PC119340E (2022) https://doi.org/10.1117/12.2611039

KEYWORDS: Skin cancer, Visualization, Tissues, Confocal microscopy, Two photon excitation microscopy, Reflectivity, Skin, Toxicity, Surgery, Second-harmonic generation

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Proceedings Article | 7 March 2022 Presentation

Non-invasive high-contrast imaging of conjunctival goblet cells by moxifloxacin-based fluorescence microscopy

Ki Hean Kim, Seonghan Kim, Jungbin Lee, Young-Ho Jung, Chang Ho Yoon

Proceedings Volume PC11941, PC119410W (2022) https://doi.org/10.1117/12.2610724

KEYWORDS: Microscopy, Luminescence, Eye models, Animal model studies, Eye, Confocal microscopy, Mouse models, Imaging devices, Image acquisition

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Proceedings Article | 7 March 2022 Presentation

Moxifloxacin-based extended depth-of-field fluorescence microscopy for real-time conjunctival goblet cell examination

Jungbin Lee, Seonghan Kim, Byeong Jae Son, Jeongho Kim, Myoung Joon Kim, Chang Ho Yoon, Hong Kyun Kim, Ki Hean Kim

Proceedings Volume PC11941, PC119410Y (2022) https://doi.org/10.1117/12.2610725

KEYWORDS: Microscopy, Luminescence, Motion models, Imaging systems, Image resolution, Real time imaging, Performance modeling, In vivo imaging, Image filtering, Human subjects

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Proceedings Article | 9 March 2020 Presentation

Fast and sensitive delineation of brain tumor with clinically compatible moxifloxacin labeling and confocal microscopy (Conference Presentation)

Seunghun Lee, Wonyeong Park, Seonghan Kim, Hoonchul Chang, Bumju Kim, Won Hyuk Jang, Younghoon Shin, Myoung Joon Kim, Euiheon Chung, Eui Hyun Kim, Kyung Hwa Lee, Ki Hean Kim

Proceedings Volume 11229, 112291F (2020) https://doi.org/10.1117/12.2546056

KEYWORDS: Tumors, Brain, Confocal microscopy, Visualization, Surgery, Tissues, Neuroimaging, Luminescence, Tumor growth modeling, Cancer

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Proceedings Article | 9 March 2020 Presentation

Real-time angiographic OCT for guiding dermal filler injection (Conference Presentation)

Jungbin Lee, SeongHan Kim, Ikjong Park, Byungho Oh, Ki Hean Kim

Proceedings Volume 11229, 112290B (2020) https://doi.org/10.1117/12.2542381

KEYWORDS: Angiography, Optical coherence tomography, Skin, Blood vessels, Arteries, Coherence (optics), Interferometry, Visualization, In vivo imaging, Mouse models

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Proceedings Article | 9 March 2020 Presentation

High-resolution tissue imaging using moxifloxacin as a clinically compatible cell labeling agent (Conference Presentation)

Ki Hean Kim, Seunghun Lee, Bumju Kim

Proceedings Volume 11244, 112441P (2020) https://doi.org/10.1117/12.2552736

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Proceedings Article | 24 April 2017 Presentation

In vivo longitudinal visualization of bone marrow engraftment process in mouse calvarium bone marrow with two-photon microscopy (Conference Presentation)

Viet Hoan Le, Seunghun Lee, Ki Hean Kim, Seungwon Lee, Seung-Woo Lee

Proceedings Volume 10069, 100690E (2017) https://doi.org/10.1117/12.2252055

KEYWORDS: Tumors, Bone, Transplantation, Visualization, Microscopy, Mouse models, In vivo imaging, Two photon excitation microscopy, Leukemia, Surgery

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Proceedings Article | 26 April 2016 Presentation

In-vivo cutaneous burn characterization and scar assay with multi-functional optical coherence tomography (Conference Presentation)

Bumju Kim, Yeorum Yoon, Viet-Hoan Le, Calvin Yoon, Ki Hean Kim

Proceedings Volume 9697, 96971C (2016) https://doi.org/10.1117/12.2214817

KEYWORDS: Optical coherence tomography, Wound healing, In vivo imaging, Angiography, Polarization, Tissues, Skin, Image processing, Blood vessels, Birefringence

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SPIE Journal Paper | 7 August 2013 Open Access

Combined two-photon microscopy and angiographic optical coherence tomography

Bumju Kim, Taejun Wang, Qingyun Li, Jutaek Nam, Sekyu Hwang, Euiheon Chung, Sungjee Kim, Ki Hean Kim

JBO, Vol. 18, Issue 08, 080502, (August 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.8.080502

KEYWORDS: Optical coherence tomography, Angiography, Ear, Cancer, In vivo imaging, Tumor growth modeling, Tissues, Animal model studies, Visualization, Two photon excitation microscopy

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SPIE Journal Paper | 4 June 2012 Open Access

In vivo imaging of human burn injuries with polarization-sensitive optical coherence tomography

Ki Hean Kim, Mark Pierce, Gopi Maguluri, B. Hyle Park, Sang June Yoon, Martha Lydon, Robert Sheridan, Johannes de Boer

JBO, Vol. 17, Issue 06, 066012, (June 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.6.066012

KEYWORDS: Birefringence, Control systems, Skin, In vivo imaging, Injuries, Optical coherence tomography, Tissues, 3D image processing, Video, Polarization

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SPIE Journal Paper | 1 December 2011 Open Access

Measurement of anisotropic reflection of flowing blood using optical coherence tomography

Kweon-Ho Nam, Sang-Joon Lee, Bosu Jeong, In Oh Jung, Hojin Ha, Ki Hean Kim

JBO, Vol. 16, Issue 12, 120502, (December 2011) https://doi.org/10.1117/12.10.1117/1.3660299

KEYWORDS: Optical coherence tomography, Reflectivity, Blood, Blood circulation, Optical alignment, Scattering, Multiple scattering, Mechanical engineering, Doppler tomography, Blood vessels

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Proceedings Article | 1 March 2011 Paper

Combined two-photon microscopy and optical coherence tomography using individually optimized sources

Bosu Jeong, Byunghak Lee, Min Seong Jang, Hyoseok Nam, Hae Koo Kim, Sang June Yoon, Junsang Doh, Sang-Joon Lee, Bo-Gie Yang, Myoung Ho Jang, Ki Hean Kim

Proceedings Volume 7903, 79030Q (2011) https://doi.org/10.1117/12.876000

KEYWORDS: Optical coherence tomography, Tissues, Intestine, Objectives, 3D image processing, Tissue optics, Visualization, Two photon excitation microscopy, Image resolution, In vivo imaging

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Proceedings Article | 18 February 2011 Paper

Laryngeal imaging with polarization-sensitive optical coherence tomography

James Burns, Ki Hean Kim, R. Rox Anderson

Proceedings Volume 7883, 78832N (2011) https://doi.org/10.1117/12.875443

KEYWORDS: Optical coherence tomography, Tissues, Tissue optics, Birefringence, Polarization, Collagen, Pathology, In vivo imaging, Natural surfaces, Visualization

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SPIE Journal Paper | 1 May 2010 Open Access

Ex vivo imaging and quantification of liver fibrosis using second-harmonic generation microscopy

Tzu-Lin Sun, Yuan Liu, Ming-Chin Sung, Hsiao-Ching Chen, Chun-Hui Yang, Vladimir Hovhannisyan, Wei-Chou Lin, Yung-Ming Jeng, Wei-Liang Chen, Ling-Ling Chiou, Guan-Tarn Huang, Ki Hean Kim, Peter So, Yang-Fang Chen, Hsuan-Shu Lee, Chen-Yuan Dong

JBO, Vol. 15, Issue 03, 036002, (May 2010) https://doi.org/10.1117/12.10.1117/1.3427146

KEYWORDS: Liver, Second-harmonic generation, Collagen, Tissues, Multiphoton microscopy, Objectives, Physics, In vivo imaging, Imaging systems, Microscopy

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SPIE Journal Paper | 1 September 2009 Open Access

Single-wavelength reflected confocal and multiphoton microscopy for tissue imaging

Wei-Liang Chen, Chen-Kuan Chou, Ming-Gu Lin, Yang-Fang Chen, Shiou-Hwa Jee, Hsian-Yuan Tan, Tsung-Hua Tsai, Ki Hean Kim, Daekeun Kim, Peter So, Sung-Jan Lin, Chen-Yuan Dong

JBO, Vol. 14, Issue 05, 054026, (September 2009) https://doi.org/10.1117/12.10.1117/1.3247157

KEYWORDS: Skin, Second-harmonic generation, Cornea, Signal detection, Confocal microscopy, Multiphoton microscopy, Tissues, Tissue optics, Refractive index, Roads

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Proceedings Article | 12 February 2007 Paper

Scanning OCT endoscope with 2-axis magnetic micromirror

Jonathan Bernstein, Tom Lee, Fran Rogomentich, Mirela Bancu, Ki Kim, Gopi Maguluri, Brett Bouma, Johannes DeBoer

Proceedings Volume 6432, 64320L (2007) https://doi.org/10.1117/12.701266

KEYWORDS: Mirrors, Microelectromechanical systems, Endoscopes, Optical coherence tomography, Scanners, Micromirrors, Magnetism, Semiconducting wafers, Etching, Raster graphics

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SPIE Journal Paper | 1 January 2007 Open Access

High-resolution whole organ imaging using two-photon tissue cytometry

Timothy Ragan, Jeremy Sylvan, Ki Kim, Hayden Huang, Karsten Bahlmann, Richard Lee, Peter So

JBO, Vol. 12, Issue 01, 014015, (January 2007) https://doi.org/10.1117/12.10.1117/1.2435626

KEYWORDS: Tissues, Heart, 3D image processing, Image resolution, Tissue optics, Biology, Microscopes, Image segmentation, Stereoscopy, Imaging systems

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Proceedings Article | 30 March 2005 Paper

Optical biopsy in high-speed handheld miniaturized multifocal multiphoton microscopy

Daekeun Kim, Ki Hean Kim, Siavash Yazdanfar, Peter So

Proceedings Volume 5700, (2005) https://doi.org/10.1117/12.597055

KEYWORDS: Biopsy, Tissue optics, Skin, Photonic crystal fibers, Multiphoton microscopy, Mirrors, Tissues, Distortion, Microscopes, Dispersion

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Proceedings Article | 21 June 2004 Paper

Development of an improved FRET-imaging two-photon microscope combining lifetime and spectral imaging

Serge Pelet, Micheal Previte, Ki Hean Kim, Peter So

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.537620

KEYWORDS: Fluorescence resonance energy transfer, Modulation, Luminescence, Imaging spectroscopy, Phase shift keying, Microscopes, Fluorescence lifetime imaging, Proteins, Algorithm development, Phase measurement

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Proceedings Article | 21 June 2004 Paper

High-speed handheld multiphoton multifoci microscopy

Daekeun Kim, Ki Kim, Siavash Yazdanfar, Peter So

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.530395

KEYWORDS: Multiphoton microscopy, Mirrors, Microscopy, Optical fibers, Imaging systems, Beam splitters, Luminescence, High speed imaging, Biopsy, Tissue optics

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Proceedings Article | 21 June 2004 Paper

Signal degradation in multiple-scattering medium: implication for single focus vs multifoci two-photon microscopy

Ki Kim, Christof Buehler, Karsten Bahlmann, Peter So

Proceedings Volume 5323, (2004) https://doi.org/10.1117/12.532396

KEYWORDS: Charge-coupled devices, Point spread functions, Scattering, Sensors, Signal detection, Light scattering, Two photon excitation microscopy, Neurons, Brain, CCD image sensors

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Proceedings Article | 17 June 2002 Paper

Multicolor two-photon scanning microscopy using a 16-channel photomultiplier

Christof Buehler, Ki Kim, Urs Greuter, Nick Schlumpf, Peter So

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.470694

KEYWORDS: Luminescence, Microscopes, Photon counting, Imaging spectroscopy, Tissue optics, Field programmable gate arrays, Microscopy, Spectrographs, Photomultipliers, Spectral resolution

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Proceedings Article | 24 April 2001 Paper

Three-dimensional image cytometer based on a high-speed two-photon scanning microscope

Ki Kim, Molly Stitt, Carrie Hendricks, Karen Almeida, Bevin Engelward, Peter So

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424559

KEYWORDS: 3D image processing, Tissues, Mirrors, Microscopes, Objectives, Skin, Scanners, Fluorescent proteins, Microscopy, Polygon scanners

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We developed a 3-D image cytometer based on two-photon scanning microscopy. The system keeps the inherent advantages from two-photon scanning microscopy: (1) The ability of imaging thick tissue samples up to a few hundred micrometers, (2) The ability to study tissue structures with subcellular resolution, (3) The ability to monitor tissue biochemistry and metabolism, and (4) The reduction of specimen photobleaching and photodamage. Therefore, 3-D image cytometer has the ability to characterize multiple cell layer specimens, in contrast with 2-D image cytometer where only single cell layer samples can be imaged. 3-D image cytometry increases its frame rate by adapting a polygonal mirror scanner and high-speed photomultiplier tubes. The current frame rate is 13 frames per second. High throughput rate is achieved by imaging multiple cell layer specimens in 3-D at a high frame rate. The throughput rate of this system is dependent on the choice of objective lenses, specimen properties, and the speed of computer-controlled specimen stage. It can be up to approximately 100 cells per second which is comparable with that of 2-D image cytometers. With the high throughput rate and deep tissue imaging capability, 3-D image cytometer has the potential for the detection of rare cellular events inside living, intact tissues. A promising application of this 3-D image cytometer is the study of mitotic recombination in tissues. Mitotic recombination is a mechanism for genetic change. Therefore it is one of causes for carcinogenesis. However, the study of this process is difficult because recombination event is rare and it occurs at a rate of one cell in 10⁵ cells. The new method for the study is (1) to engineer transgenic mice whose cells will express fluorescence in the presence of mitotic recombination, (2) to detect cells which have undergone mitotic recombination with 3-D image cytometry. The estimated time required to quantify spontaneous recombination rate is approximately within a few hours in the case that the mutation occurs at a rate of 1/10⁵. The ability of this 3-D image cytometer to resolve tissue structures at video rate was demonstrated in the study of ex vivo human skin dermal structure. 25 X 25 section images were taken by shifting the acquisition region with computer- controlled specimen stage. Wide area images were reconstructed by combining each image sections. The size of complete wide area images is approximately 25 mm X 25 mm. We further performed experiments to verify this cytometer's ability for population statistics measurement. We prepared cell cultures containing a mixture of cells expressing cyan and yellow fluorescent proteins. These cell cultures with mixing ratio ranging from 1/10 up to 1/10⁵ were imaged. Experimental results show that the presence of a few rare cells in large pool of the other cells can be quantitatively measured. We also imaged a punched ear specimen from a transgenic mouse which carries green fluorescent protein. The data demonstrates that this system can resolve a single green fluorescent cells in the tissue.