Dr. Marlon S. Mathews Profile

Dr. Marlon S. Mathews

Post Doctural Scholar at Univ of California Irvine

SPIE Involvement:

Author

Publications (18)

Proceedings Article | 10 March 2015 Paper

Hemodynamic and morphologic responses in mouse brain during acute head injury imaged by multispectral structured illumination

Boris Volkov, Marlon Mathews, David Abookasis

Proceedings Volume 9305, 93050L (2015) https://doi.org/10.1117/12.2078711

KEYWORDS: Brain, Injuries, Head, Hemodynamics, Scattering, Spatial frequencies, Neuroimaging, Tissue optics, Multispectral imaging, Light scattering

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Proceedings Article | 5 March 2014 Paper

Monitoring closed head injury induced changes in brain physiology with orthogonal diffuse near-infrared reflectance spectroscopy

David Abookasis, Ariel Shochat, Marlon Mathews

Proceedings Volume 8928, 89280J (2014) https://doi.org/10.1117/12.2035438

KEYWORDS: Injuries, Brain, Scattering, Absorption, Tissue optics, Head, Light, Chromophores, Diffuse reflectance spectroscopy, Light scattering

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SPIE Journal Paper | 12 November 2013 Open Access

Closed head injury-induced changes in brain pathophysiology assessed with near-infrared structured illumination in a mouse model

David Abookasis, Boris Volkov, Marlon Mathews

JBO, Vol. 18, Issue 11, 116007, (November 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.11.116007

KEYWORDS: Brain, Injuries, Scattering, Head, Absorption, Light scattering, Brain mapping, Tissues, Oxygen, Hemodynamics

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Use of near-infrared (NIR) structured illumination technique has recently received great interest in biomedical research and clinical studies because of its ability to perform wide-field imaging and quantitatively map changes in tissue hemodynamic properties and morphological features in a noncontact and scan-free fashion. We report on the feasibility of using the same to quantitatively monitor and map changes in brain optical properties and physiological parameters pre- and post-closed head injury in a mouse model. Five anesthetized male mice underwent head injury by weight-drop model using a ∼50-g cylindrical metal object falling from a height of 90 cm onto the intact scalp. During experiments, NIR structured illumination was projected on the mouse head at two spatial frequencies and six different NIR wavelengths. A CCD camera positioned perpendicular to the head recorded the diffuse-reflected light. Computer analysis performed off-line on the captured data reveals spatiotemporal changes in the distribution of brain tissue absorption and reduced scattering coefficients. Using Beer’s law and Mie theory, hemodynamic (hemoglobin, oxygen saturation, and lipids) and morphological (scattering amplitude and power) changes up to 1-h post-trauma were observed in comparison with baseline measurements. Functional maps of different brain properties were also generated. Following injury, we found difference in both brain hemodynamic and morphologic properties with respect to baseline levels, where in some properties, this difference was considered statistically significant. Specifically, a t -test indicates a substantial decrease in oxyhemoglobin (HbO2) concentration and tissue oxygen saturation (StO2) post-injury (p < 0.01 and p < 0.001, respectively). Overall, our preliminary results demonstrate the potential application of NIR structured illumination technique to track and spatially map changes in intact mouse brain pathophysiological parameters following head injury.

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SPIE Journal Paper | 4 April 2013 Open Access

Monitoring hemodynamic and morphologic responses to closed head injury in a mouse model using orthogonal diffuse near-infrared light reflectance spectroscopy

David Abookasis, Ariel Sochat, Marlon Mathews

JBO, Vol. 18, Issue 04, 045003, (April 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.4.045003

KEYWORDS: Scattering, Injuries, Brain, Chromophores, Head, Hemodynamics, Absorption, Light scattering, Sensors, Tissues

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The authors’ aim is to assess and quantitatively measure brain hemodynamic and morphological variations during closed-head injury (CHI) in mice using orthogonal diffuse near-infrared reflectance spectroscopy (o-DRS). CHI is a type of injury to the head that does not penetrate the skull. Usually, it is caused by mechanical blows to the head and frequently occurs in traffic accidents, falls, and assaults. Measurements of brain optical properties, namely absorption and reduced scattering coefficients in the wavelength range from 650 to 1000 nm were carried out by employing different source–detector distance and locations to provide depth sensitivity on an intact scalp over the duration of the whole experiment. Furthermore, alteration in both cortical hemodynamics and morphologic markers, i.e., scattering power and amplitude properties were derived. CHI was induced in anesthetized male mice by a weight-drop model using ∼50 g cylindrical metal falling from a height of 90 cm onto the intact scalp producing an impact of 4500 g cm. With respect to baseline, difference in brain physiological properties was observed following injury up to 1 h post-trauma. Additionally, the reduced scattering spectral shapes followed Mie scattering theory was quantified and clearly shows changes in both scattering amplitude and power from baseline indicating structural variations likely from evolving cerebral edema during CHI. We further demonstrate high correlation between scattering amplitude and scattering power, with more than 20% difference in slope in comparison to preinjury. This result indicates the possibility of using the slope also as a marker for detection of structural changes. Finally, experiments investigating brain function during the first 20 min postinjury were conducted and changes in chromophore concentrations and scattering were observed. Overall, our experiments demonstrate the potential of using the proposed technique as a valuable quantitative noninvasive tool for monitoring brain physiology following CHI injury at the bedside and/or at the field.

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Proceedings Article | 8 March 2013 Paper

Orthogonal diffuse near-infrared reflectance spectroscopy allows to assess cerebral dysfunction and temperature variations following heatstroke on a mouse model

David Abookasis, Elad Zafrir, Elimelech Nesher, Albert Pinhasov, Shmuel Sternklar, Marlon Mathews

Proceedings Volume 8565, 85654N (2013) https://doi.org/10.1117/12.2005826

KEYWORDS: Temperature metrology, Brain, Scattering, Absorption, Absorbance, Diffuse reflectance spectroscopy, Optical properties, Tissue optics, Reflectivity, Spectroscopy

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SPIE Journal Paper | 8 November 2012 Open Access

Errata: Diffuse near-infrared reflectance spectroscopy during heatstroke in a mouse model: pilot study

David Abookasis, Elad Zafrir, Elimelech Nesher, Albert Pinhasov, Shmuel Sternklar, Marlon Mathews

JBO, Vol. 17, Issue 11, 119801, (November 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.11.119801

KEYWORDS: Reflectance spectroscopy, Mouse models, Biomedical optics, Electronics engineering, Molecular biology, Surgery

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SPIE Journal Paper | 18 October 2012 Open Access

Diffuse near-infrared reflectance spectroscopy during heatstroke in a mouse model: pilot study

David Abookasis, Elad Zafrir, Elimelech Nesher, Albert Pinhasov, Shmuel Sternklar, Marlon M.

JBO, Vol. 17, Issue 10, 105009, (October 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.10.105009

KEYWORDS: Brain, Temperature metrology, Scattering, Absorption, Tissue optics, Tissues, Absorbance, Reflectivity, Diffuse reflectance spectroscopy, Reflectance spectroscopy

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Heatstroke, a form of hyperthermia, is a life-threatening condition characterized by an elevated core body temperature that rises above 40°C (104°F) and central nervous system dysfunction that results in delirium, convulsions, or coma. Without emergency treatment, the victim lapses into a coma and death soon follows. The study presented was conducted with a diffuse reflectance spectroscopy (DRS) setup to assess the effects of brain dysfunction that occurred during heatstroke in mice model (n=6 ). It was hypothesized that DRS can be utilized in small animal studies to monitor change in internal brain tissue temperature during heatstroke injury since it induces a sequence of pathologic changes that change the tissue composition and structure. Heatstroke was induced by exposure of the mice body under general anesthesia, to a high ambient temperature. A type of DRS in which the brain tissue was illuminated through the intact scalp with a broadband light source and diffuse reflected spectra was employed, taking in the spectral region between 650 and 1000 nm and acquired at an angle of 90 deg at a position on the scalp ∼12 mm from the illumination site. The temperature at the onset of the experiment was ∼34°C (rectal temperature) with increasing intervals of 1°C until mouse death. The increase in temperature caused optical scattering signal changes consistent with a structural alteration of brain tissue, ultimately resulting in death. We have found that the peak absorbance intensity and its second derivative at specific wavelengths correlate well with temperature with an exponential dependence. Based on these findings, in order to estimate the influence of temperature on the internal brain tissue a reflectance-temperature index was established and was seen to correlate as well with measured temperature. Overall, results indicate variations in neural tissue properties during heatstroke and the feasibility to monitor and assess internal temperature variations using DRS.

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SPIE Journal Paper | 3 May 2012 Open Access

Photochemical internalization of bleomycin for glioma treatment

Marlon Mathews, En-Chung Shih, Genesis Zamora, Chung-Ho Sun, Henry Hirschberg, Joseph Blickenstaff, Van Vo, Steen Madsen

JBO, Vol. 17, Issue 5, 058001, (May 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.5.058001

KEYWORDS: Tumors, Photodynamic therapy, Brain, Control systems, Toxicity, Blood brain barrier, In vitro testing, Cell death, Two photon excitation microscopy, Plasma

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Proceedings Article | 9 February 2012 Paper

Enhanced gene transfection by photochemical internalization of protomine sulfate/DNA complexes

Henry Hirschberg, Marlon Mathews, En-Chung Shih, Steen Madsen, Young Jik Kwon

Proceedings Volume 8207, 82074S (2012) https://doi.org/10.1117/12.911938

KEYWORDS: Tumors, Cancer, Photodynamic therapy, Stem cells, Green fluorescent protein, Picosecond phenomena, Brain, Macromolecules, Laser therapeutics, Proteins

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Proceedings Article | 18 February 2011 Paper

Neuro-endovascular optical coherence tomography imaging: clinical feasibility and applications

Marlon Mathews, Jianping Su, Esmaeil Heidari, Mark Linskey, Zhongping Chen

Proceedings Volume 7883, 788341 (2011) https://doi.org/10.1117/12.876236

KEYWORDS: Optical coherence tomography, Arteries, Coherence imaging, In vivo imaging, Image segmentation, Pathology, Tissue optics, Optical imaging, Visualization, Image resolution

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SPIE Journal Paper | 1 March 2009 Open Access

Imaging cortical absorption, scattering, and hemodynamic response during ischemic stroke using spatially modulated near-infrared illumination

David Abookasis, Christopher Lay, Marlon Mathews, Mark Linskey, Ron Frostig, Bruce Tromberg

JBO, Vol. 14, Issue 02, 024033, (March 2009) https://doi.org/10.1117/12.10.1117/1.3116709

KEYWORDS: Scattering, Absorption, Brain, Tissue optics, Light scattering, Ischemia, Tissues, Chromophores, Spatial frequencies, Optical properties

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Proceedings Article | 5 March 2008 Paper

Imaging treated brain aneurysms in vivo using optical coherence tomography

Jianping Su, Marlon Mathews, Chiedozie Nwagwu, Ahmad Edris, Binh Nguyen, Mehrzad Heidari, Mark Linskey, Zhongping Chen

Proceedings Volume 6847, 684732 (2008) https://doi.org/10.1117/12.766657

KEYWORDS: Optical coherence tomography, Brain, Blood, In vivo imaging, Angiography, Neuroimaging, Cerebrovascular diseases, Visualization, Neck, Light sources

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Proceedings Article | 26 February 2008 Open Access

Using NIR spatial illumination for detection and mapping chromophore changes during cerebral edema

David Abookasis, Marlon Mathews, Christopher Owen, Devin Binder, Mark Linskey, Ron Frostig, Bruce Tromberg

Proceedings Volume 6842, 68422U (2008) https://doi.org/10.1117/12.760516

KEYWORDS: Chromophores, Brain, Tissues, Near infrared, Modulation, Brain mapping, Oxygen, Tissue optics, Light scattering, Image processing

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Proceedings Article | 26 February 2008 Paper

The effect of motexafin gadolinium on ALA photodynamic therapy in glioma spheroids

Marlon Mathews, Rogelio Sanchez, Chung-Ho Sun, Steen Madsen, Henry Hirschberg

Proceedings Volume 6842, 68422N (2008) https://doi.org/10.1117/12.760260

KEYWORDS: Photodynamic therapy, Tumors, Brain, Gadolinium, Cancer, Tissue optics, In vitro testing, Magnesium, Tissues, In vivo imaging

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Proceedings Article | 29 March 2007 Open Access

Modulated imaging: a novel method for quantifying tissue chromophores in evolving cerebral ischemia

David Abookasis, Marlon Mathews, Chistopher Lay, Ron Frostig, Bruce Tromberg

Proceedings Volume 6511, 65110A (2007) https://doi.org/10.1117/12.713470

KEYWORDS: Brain, Ischemia, Neuroimaging, Chromophores, Modulation, Tissues, Tissue optics, Oxygen, Arteries, Brain mapping

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Proceedings Article | 23 March 2007 Paper

Increased brain edema following 5-aminolevulinic acid mediated photodynamic in normal and tumor bearing rats

Henry Hirschberg, Even Angell-Petersen, Signe Spetalen, Marlon Mathews, Steen Madsen

Proceedings Volume 6424, 64242B (2007) https://doi.org/10.1117/12.705439

KEYWORDS: Tumors, Brain, Photodynamic therapy, Blood brain barrier, Magnetic resonance imaging, Tissues, Neuroimaging, Distance measurement, Luminescence, Skin

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Introduction: Failure of treatment for high grade gliomas is usually due to local recurrence at the site of surgical resection indicating that a more aggressive form of local therapy, such as PDT, could be of benefit. PDT causes damage to both tumor cells as well as cerebral blood vessels leading to degradation of the blood brain barrier with subsequent increase of brain edema. The increase in brain edema following ALA-PDT was evaluated in terms of animal survival, histopatological changes in normal brain and tumor tissue and MRI scanning. The effect of steroid treatment, to reduce post-treatment PDT induced edema, was also examined. Methods:Tumors were established in the brains of inbred BD-IX and Fisher rats. At various times following tumor induction the animals were injected with ALA ip. and four hours later light treatment at escalating fluences and fluence rates were given. Nontumor bearing control animals were also exposed to ALA-PDT in a similar manner to evaluate damage to normal brain and degree of blood brain barrier (BBB) disruption. Results: Despite a very low level of PpIX production in normal brain, with a 200:1 tumor to normal tissue selectivity ratio measured at a distance of 2 mm from the tumor border, many animals succumbed shortly after treatment. A total radiant energy of 54 J to non-tumor bearing animals resulted in 50% mortality within 5 days of treatment. Treatment of tumor bearing animals with moderate fluence levels produced similar brain edema compared to higher fluence levels. ALA PDT in nontumor bearing animals produced edema that was light dose dependent. PDT appeared to open the BBB for a period of 24-48 hrs after which it was restored. The addition of post operative steroid treatment reduced the incident of post treatment morbidity and mortality. Conclusions: T2 and contrast enhanced T1 MRI scanning proved to be a highly effective and non-evasive modality in following the development of the edema reaction and the degree and time course of BBB dysfunction thus allowing the use of fewer animals.